Effect of extract of Quzhou Aurantii Fructus on hepatic inflammation and NF-κB/NLRP3 inflammasome pathway in CCl4-induced liver fibrosis miceChinese Full TextEnglish Full Text (MT)
WANG Si-wei;LAN Tian;ZHENG Fang;LEI Mei-kang;ZHANG Feng;Quzhou People′s Hospital;Quzhou Customs Technical Center;
Abstract: To study the effect and mechanism of extract of Quzhou Aurantii Fructus(QAF) on liver inflammation in CCl4-induced liver fibrosis mice. Totally 60 C57 BL/6 male mice were randomly divided into control group(distilled water, oral), model group(distilled water, oral), colchicines group(Col, colchicines 2 mg·kg-1·d-1, oral), low-dose QAF group(QAF-L, QAF 100 mg·kg-1·d-1, oral) and high-dose QAF group(QAF-H, QAF 300 mg·kg-1·d-1, oral) by random number table method. The model group and each administration group were injected with carbon tetrachloride(CCl4) 1 mL·kg-1(CCl4-olive oil 1∶4), twice a week, totally 6 weeks. After the last administration, the mice were sacrificed, and serum and liver tissue were collected. Serum ALT and AST levels were measured in each group to observe the liver function of mice. The pathological changes and inflammatory cell infiltration in liver were observed by HE staining and F4/80 immunohistochemical staining. The mRNA expressions of TNF-α, IL-18 and IL-1β were detected by RT-PCR. The protein expressions of IκBα, p-IKKα/β, p-p65, NLRP3, caspase-1 and cleaved caspase-1 were analyzed by Western blot. The results showed that QAF significantly reduced serum ALT and AST levels, and alleviated the degree of liver damage.The results of immunohistochemistry showed that QAF significantly reduced liver inflammatory cell infiltration in liver fibrosis mice. The results of RT-PCR and Western blot showed that QAF significantly inhibited mRNA expressions of TNF-α, IL-18 and IL-1β in liver of fibrosis mice. QAF also suppressed the degradation of IκBα protein and reduced p-IKKα/β, p-p65, NLRP3 and cleaved caspase-1 protein expressions. In conclusion, QAF improves CCl4-induced liver fibrosis in mice. The mechanism may be related to the inhibition of NF-κB/NLRP3 inflammasome-mediated inflammation signaling pathway.
- DOI:
10.19540/j.cnki.cjcmm.20201201.401
- Series:
(E) Medicine & Public Health
- Subject:
Traditional Chinese Medicinal Herbs
- Classification Code:
R285.5
- Mobile Reading
Read on your phone instantly
Step 1Scan QR Codes
"Mobile CNKI-CNKI Express" App
Step 2Open“CNKI Express”
and click the scan icon in the upper left corner of the homepage.
Step 3Scan QR Codes
Read this article on your phone.
- HTML
- BETABETAEnglish HTML (MT)
- CAJ Download
- PDF Download
Download the mobile appuse the app to scan this coderead the article.
Tips: Please download CAJViewer to view CAJ format full text.
Download: 1152 Page: 1474-1479 Pagecount: 6 Size: 2590K
Citation Network
Related Literature
- Similar Article
- Reader Recommendation
- Associated Author