Research Article
Investigating the Effect of Mesenchymal Stem Cells on Peripheral Nerve Repair with Quantitative Methods
Abstract
Objective: The aim was to research the possible effects of bone marrow stromal cells on the effective method for peripheral nerve injuries of end-in-end repair for sciatic nerves using quantitative histologic methods.
Methods: Five adult male New Zealand rabbits had both sciatic nerves cut and then the end-in-end repair technique performed. Then while nothing was administered to the left side, the right side had autologous mesenchymal stem cell injection from previously cultured material administered. Four weeks later the repair area was removed, and sections were stained with S100 and CD31 antibodies. Stained sections were investigated for Schwann cells and vessels. Evaluations of the left and right sides were made by comparing their Schwann cell density and vessel density determined by estimating their volume fractions, separately.
Results: The repair areas on both sides showed no significant differences for the degree of immunoreactivity for S100 and CD31 (p=0.003). But the volume fractions of Schwann cells and vessels within the repair areas were found to be increased for the cell-treated nerves, but statistically there was no difference for vessels (p=0.073).
Conclusion: These results support the findings already reported in the literature, that increased Schwann cell density will probably promote regeneration of the peripheral nerve.
Supporting Institution
Ondokuz Mayıs University
Project Number
T573
Thanks
This study was supported by the Project Management office of Ondokuz Mayıs University (Project No. T573)
References
- Choi BH, Zhu SJ, Kim BY, Huh JY, Lee SH, Jung JH. Transplantation of cultured bone marrow stromal cells to improve peripheral nerve regeneration. Int J Oral Maxillofac Surg. 2005; 34(5): 537-542.
- Cuevas P, Carceller F, Dujovny M, Garcia-Gómez I, Cuevas B, González-Corrochano R, Diaz-González D, Reimers D. Peripheral nerve regeneration by bone marrow stromal cells. Neurol Res. 2002; 24(7): 634-8.
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- Dezawa M. Central and peripheral nerve regeneration by transplantation of Schwann cells and transdifferentiated bone marrow stromal cells. Anatomical Science International. 2002; 77(1): 12-25.
- Fernandes M, Valente SG, Fernandes MJS, Félix EP, Mazzacoratti Mda G, Scerni DA, dos Santos JB, Leite VM, Faloppa F. Bone marrow cells are able to increase vessels number during repair of sciatic nerve lesion. J Neurosc Metods. 2008; 170(1): 16-24.
- Howard CV and Reed MG. Unbiased Stereology, Three-Dimensional Measurement in Microscopy. First edition. BIOS scientific publishers. 1998.
- Im Gl, Kim DY, Shin JH, Hyun CW, Cho WH. Repair of cartilage defect in the rabbit with cultured mesenchymal stem cells from bone marrow. J Bone Joint Surg Br. 2001; 83(2): 289-294.
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- Kernan NA, Flomenberg N, Dupont B, O'Reilly RJ. Graft rejection in recipients of T-cell-depleted HLA-nonidentical marrow transplants for leukemia. Identification of host-derived antidonor allocytotoxic T lymphocytes. Transplantation. 1987; 43(6): 842-847.
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- Orlic D, Kajstura J, Chimenti S. Jakoniuk I, Anderson SM, Li B, Pickel J, McKay R, Nadal-Ginard B, Bodine DM, Leri A, Anversa P. Bone marrow cells regenerate infarcted myocardium. Nature. 2001; 410(6829): 701-705.
- Perin EC, Dohmann HF, Brojevic R, Silva SA, Sousa AL, Mesquita CT, Rossi MI, Carvalho AC, Dutra HS, Dohmann HJ, Silva GV, Belém L, Vivacqua R, Rangel FO, Esporcatte R, Geng YJ, Vaughn WK, Assad JA, Mesquita ET, Willerson JT. Transendocardial, autologous bone marrow cell transplantation for severe, chronic ischemic heart failure. Circulation. 2003; 107(18): 2294-2302.
- Siemionow M, Tetik C, Ozer K, Ayhan S, Siemionow K, Browne E. Epineural sleeve neurrorrhaphy: surgical technique and functional results-preliminary report. Annals of Plastic Surgery. 2002; 48(3): 281-285.
- Tohill M, Mantovani C, Wiberg M, Terenghi G. Rat bone marrow mesenchymal stem cells express glial markers and stimulate nerve regeneration. Neuroscience Letters. 2004; 362(3): 200-203.
- Wang J, Ding F, Gu Y, Liu J, Gu X. Bone marrow mesenchymal stem cells promote cell proliferation and neurotrophic function of Schwann cells in vitro and in vivo. Brain Res. 2009; 1262: 7-15.
- Zhang P, He X, Liu K, Zhao F, Fu Z, Zhang D, Zhang Q, Jiang B. Bone marrow stromal cells differentiated into functional Schwann cells in injured rats sciatic nerve. Artif. Cells Blood Substit. Immobil. Biotechnol. 2004; 32(4): 509-518.
Year 2020,
Volume: 6 Issue: 1, 65 - 69, 30.04.2020
Abstract
Project Number
T573
References
- Choi BH, Zhu SJ, Kim BY, Huh JY, Lee SH, Jung JH. Transplantation of cultured bone marrow stromal cells to improve peripheral nerve regeneration. Int J Oral Maxillofac Surg. 2005; 34(5): 537-542.
- Cuevas P, Carceller F, Dujovny M, Garcia-Gómez I, Cuevas B, González-Corrochano R, Diaz-González D, Reimers D. Peripheral nerve regeneration by bone marrow stromal cells. Neurol Res. 2002; 24(7): 634-8.
- Dezawa M, Takahashi I, Esaki M, Takano M, Sawada H. Sciatic nerve regeneration in rats induced by transplantation of in vitro differentiated bone-marrow stromal cells. Euro J Neuros. 2001; 14(11): 1771-1776.
- Dezawa M. Central and peripheral nerve regeneration by transplantation of Schwann cells and transdifferentiated bone marrow stromal cells. Anatomical Science International. 2002; 77(1): 12-25.
- Fernandes M, Valente SG, Fernandes MJS, Félix EP, Mazzacoratti Mda G, Scerni DA, dos Santos JB, Leite VM, Faloppa F. Bone marrow cells are able to increase vessels number during repair of sciatic nerve lesion. J Neurosc Metods. 2008; 170(1): 16-24.
- Howard CV and Reed MG. Unbiased Stereology, Three-Dimensional Measurement in Microscopy. First edition. BIOS scientific publishers. 1998.
- Im Gl, Kim DY, Shin JH, Hyun CW, Cho WH. Repair of cartilage defect in the rabbit with cultured mesenchymal stem cells from bone marrow. J Bone Joint Surg Br. 2001; 83(2): 289-294.
- Ishikawa N, Suzuki Y, Dezawa M, Kataoka K, Ohta M, Cho H, Ide C. Peripheral nerve regeneration by transplantation of BMSC-derived Schwann cells as chitosan gel sponge scaffolds. J Biomed Mater Res A. 2009; 89(4): 1118-1124.
- Kernan NA, Flomenberg N, Dupont B, O'Reilly RJ. Graft rejection in recipients of T-cell-depleted HLA-nonidentical marrow transplants for leukemia. Identification of host-derived antidonor allocytotoxic T lymphocytes. Transplantation. 1987; 43(6): 842-847.
- Mahay D, Terenghi G, Shawcross SG. Schwann cell mediated trophic effects by differentiated mesenchymal stem cells. Experimental Cell Res. 2008; 314(14): 2692-2701.
- Okama T, Lebkowski J, Schain L, Harvey M. Tricot G, Srour E, Meyers WG, Burnett A, Sniecinski I, O'Reilly RJ. The AIS CELLector: A new technology for stem cell purification. Prog Clin Biol Res. 1992; 377: 487-502.
- Orlic D, Kajstura J, Chimenti S. Jakoniuk I, Anderson SM, Li B, Pickel J, McKay R, Nadal-Ginard B, Bodine DM, Leri A, Anversa P. Bone marrow cells regenerate infarcted myocardium. Nature. 2001; 410(6829): 701-705.
- Perin EC, Dohmann HF, Brojevic R, Silva SA, Sousa AL, Mesquita CT, Rossi MI, Carvalho AC, Dutra HS, Dohmann HJ, Silva GV, Belém L, Vivacqua R, Rangel FO, Esporcatte R, Geng YJ, Vaughn WK, Assad JA, Mesquita ET, Willerson JT. Transendocardial, autologous bone marrow cell transplantation for severe, chronic ischemic heart failure. Circulation. 2003; 107(18): 2294-2302.
- Siemionow M, Tetik C, Ozer K, Ayhan S, Siemionow K, Browne E. Epineural sleeve neurrorrhaphy: surgical technique and functional results-preliminary report. Annals of Plastic Surgery. 2002; 48(3): 281-285.
- Tohill M, Mantovani C, Wiberg M, Terenghi G. Rat bone marrow mesenchymal stem cells express glial markers and stimulate nerve regeneration. Neuroscience Letters. 2004; 362(3): 200-203.
- Wang J, Ding F, Gu Y, Liu J, Gu X. Bone marrow mesenchymal stem cells promote cell proliferation and neurotrophic function of Schwann cells in vitro and in vivo. Brain Res. 2009; 1262: 7-15.
- Zhang P, He X, Liu K, Zhao F, Fu Z, Zhang D, Zhang Q, Jiang B. Bone marrow stromal cells differentiated into functional Schwann cells in injured rats sciatic nerve. Artif. Cells Blood Substit. Immobil. Biotechnol. 2004; 32(4): 509-518.
There are 17 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Research articles |
| Authors | |
| Project Number | T573 |
| Publication Date | April 30, 2020 |
| Published in Issue | Year 2020 Volume: 6 Issue: 1 |
e-ISSN 2149-7796