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Research Article

Agricultural Science Digest, volume 41 issue 4 (december 2021) : 560-565

Genetic Diversity of Natural Morchella spp. from the Southern Gansu Province Based on rDNA-ITS, China

L. Wang, Q.P. Zhou, J.M. Liu, G.H. Shi
1Department of Biology, Enrollment and Employment Office, Tangshan Normal University, Tangshan-063 000, Hebei Province, China. 
Cite article:- Wang L., Zhou Q.P., Liu J.M., Shi G.H. (2021). Genetic Diversity of Natural Morchella spp. from the Southern Gansu Province Based on rDNA-ITS, China. Agricultural Science Digest. 41(4): 560-565 . doi: 10.18805/ag.D-276.

ABSTRACT

Background: China is a mainland country rich in natural Morchella spp. resources. At present, about half of the natural Morchella spp. in the world has been recorded in China. The current study was aimed at the classification and cultivation of Morchella spp. This study provided a more accurate molecular trait for the systematic classification of Morchella spp. in southern Gansu Province, China.
Methods: From April to May 2019. Based on the molecular biological analysis of 16 natural Morchella spp. strains collected from the southern Gansu province, China. the ITS fragments between transcripts of the rDNA gene were amplified by PCR and sequenced.
Result: The results of BLAST comparison according to GenBank showed that the 16 tested strains of Morchella spp. were classified into 5 species, which were M.angusticeps, M.esculenta, M.elata, M.crapssipes and M.conica. According to the molecular evolutionary trees constructed by the Maximum-Parsimony method (MP) and the Neighbour-Joining method (NJ), the topological structures of the two molecular evolutionary trees were similar and the Bootstrap verification had a high support rate, indicating that the phylogenetic relationship had high credibility.

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