Candida albicans 와 세균의 혼합 배양이C. albicans 에 미치는 영향

@article{ART001842553},
author={ 김태형 and 이미경 },
title={Candida albicans 와 세균의 혼합 배양이C. albicans 에 미치는 영향},
journal={대한의진균학회지},
issn={1226-4709},
year={2013},
volume={18},
number={4},
pages={83-89},
doi={10.17966/kjmm.2013.18.4.83},
url={http://dx.doi.org/10.17966/kjmm.2013.18.4.83}

TY - JOUR
AU - 김태형
AU - 이미경
TI - Candida albicans 와 세균의 혼합 배양이C. albicans 에 미치는 영향
T2 - 대한의진균학회지
PY - 2013
VL - 18
IS - 4
PB - 대한의진균학회
SP - 83-89
SN - 1226-4709
AB - Background: Fungal-bacterial interactions are ubiquitous. Implanted medical devices, includingurinary bladder catheters, are particularly susceptible to colonization by Candida spp. and opportunisticbacterial pathogens. Less is known about the fungal side of fungal-bacterial interactions. Objective: The aim of the present study was to evaluate the effect of mixed culture on C. albicansand bacteria. Methods: After C. albicans was incubated either alone or in mixed culture with bacteria (Escherichiacoli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcusaureus, S. epidermidis, Enterococcus faecalis, and E. faecium) in blood agar plate, colony count anddiameter were read. Minimum inhibitory concentration (MIC) of C. albicans to fluconazole, itraconazole,and voriconazole were tested by broth microdilution method of the Clinical and Laboratory StandardsInstitute (CLSI) M27-A3. The expression of target gene (ERG11) was analyzed by RT-PCR usingreal-time PCR system. Results: When C. albicans and P. aeruginosa were mixed cultured, the colony count of C. albicanssignificantly decreased (p=0.021). The colony size, MIC, and ERG11 mRNA expression were nosignificant differences between single and mixed culture. Conclusions: Further investigations are warranted to understand the fungal-bacterial interaction inorder to aid in the design of new strategies for the treatment and prevention of infections.
KW - Candida albicans, Bacteria, Interaction, Mixed culture
DO - 10.17966/kjmm.2013.18.4.83
UR - http://dx.doi.org/10.17966/kjmm.2013.18.4.83
ER -

김태형 and 이미경 (2013). Candida albicans 와 세균의 혼합 배양이C. albicans 에 미치는 영향. 대한의진균학회지, 18( 4), 83- 89.

김태형 and 이미경 . 2013, “Candida albicans 와 세균의 혼합 배양이C. albicans 에 미치는 영향”, 대한의진균학회지, vol. 18, no. 4, pp. 83-89. Available from: doi:10.17966/kjmm.2013.18.4.83

김태형 이미경 et al. “Candida albicans 와 세균의 혼합 배양이C. albicans 에 미치는 영향” 대한의진균학회지 18.4 pp. 83-89 (2013): 83.

김태형 , 이미경 . Candida albicans 와 세균의 혼합 배양이C. albicans 에 미치는 영향 대한의진균학회지 [Internet]. 2013; 18( 4), : 83-89. Available from: doi:10.17966/kjmm.2013.18.4.83

김태형 and 이미경 . “Candida albicans 와 세균의 혼합 배양이C. albicans 에 미치는 영향” 대한의진균학회지 18, no.4 (2013): 83-89. doi: 10.17966/kjmm.2013.18.4.83

홈 권호 목록 논문 목록 논문 상세

Candida albicans 와 세균의 혼합 배양이C. albicans 에 미치는 영향

대한의진균학회지

약어 : 의진균지

2013, vol.18, no.4, pp.83 - 89

DOI : 10.17966/kjmm.2013.18.4.83

발행기관 : 대한의진균학회

연구분야 : 미생물학

김태형¹ , 이미경²

¹중앙대학교

²중앙대학교

인용한 논문 수 : - 서지 간략 보기

초록

Background: Fungal-bacterial interactions are ubiquitous. Implanted medical devices, includingurinary bladder catheters, are particularly susceptible to colonization by Candida spp. and opportunisticbacterial pathogens. Less is known about the fungal side of fungal-bacterial interactions. Objective: The aim of the present study was to evaluate the effect of mixed culture on C. albicansand bacteria. Methods: After C. albicans was incubated either alone or in mixed culture with bacteria (Escherichiacoli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcusaureus, S. epidermidis, Enterococcus faecalis, and E. faecium) in blood agar plate, colony count anddiameter were read. Minimum inhibitory concentration (MIC) of C. albicans to fluconazole, itraconazole,and voriconazole were tested by broth microdilution method of the Clinical and Laboratory StandardsInstitute (CLSI) M27-A3. The expression of target gene (ERG11) was analyzed by RT-PCR usingreal-time PCR system. Results: When C. albicans and P. aeruginosa were mixed cultured, the colony count of C. albicanssignificantly decreased (p=0.021). The colony size, MIC, and ERG11 mRNA expression were nosignificant differences between single and mixed culture. Conclusions: Further investigations are warranted to understand the fungal-bacterial interaction inorder to aid in the design of new strategies for the treatment and prevention of infections.

키워드

Candida albicans, Bacteria, Interaction, Mixed culture

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