Abstract
Purpose: We investigated the effects of Quercetin and Coenzyme Q10 antioxidant, on gentamicin-induced renal failure in rats.
Materials and Methods: The rats were given gentamicin (100 mg/kg/day, i.m., once a day), gentamicin (100 mg/kg/day, i.m.once a day) + Quercetin (15 mg/kg i.p. once a day) and gentamicin (100 mg/kg/day, i.m.) + Coenzyme Q10 (8 mg/kg i.m. once a day).
Results: In control the BUN value was 26.8 ± 0.8 (mg/100 mL); whereas, it was 117.3 ± 15.4 in gentamicin group. Renal histopathologic examination confirmed acute tubular necrosis in this group. In rats treated with gentamicin + Quercetin and gentamicin + Coenzyme Q10, a partial improvement in biochemical and histologic parameters was observed. BUN values were 117.3 ± 15.4, 44.0 ± 8.3, 47.8 ± 13.8 in gentamicin, gentamicin plus Quercetin and gentamicin plus Coenzyme Q10 treated groups, respectively. Creatinine values were 4.3 ± 0.6, 0.8 ± 0.1, 1.5 ± 0.4 in gentamicin, gentamicin plus Quercetin and gentamicin plus Coenzyme Q10 treated groups, respectively.
Conclusion: These results suggest that the administration of Quercetin and Coenzyme Q10 may have a protective effect on gentamicin-induced nephrotoxicity in rats.
Supporting Institution
Çukurova Üniversitesi
Project Number
2006/BAP 31
Thanks
Acknowledgements: Çukurova University Research Foundation TF/2006/BAP 31
References
- 1. Vaishnu Devi D, Viswanathan P. Sulphated polysaccharide from Sargassum myriocystum confers protection against gentamicin-induced nephrotoxicity in adult zebrafish. Environ Toxicol Pharmacol. 2019 23;72:103269. doi: 10.1016/j.etap.2019.103269.2. Abdel-Raheem IT, Abdel-Ghany AA, Mohamed GA. Protective effect of Quercetin against gentamisin-induced nephrotoxicity in rats. Biol. Pharm. Bull 2009; 32: 61-7. doi.org/10.1248/bpb.32.61.3. Rasheed HA, Al-Kuraishy HM, Al-Gareeb AI. Rosuvastatin Attenuates acute nephrotoxicity through modulation of oxidative stress in Sprague Dawley rats. J Pak Med Assoc. 2019;69(8): S98-S102. The 14th Scientific International Conference.4. Al-Kuraishy HM, Al-Gareeb AI, Al-Naimi MS. Renoprotective effect of irbesartan in a rat model of gentamicin-induced nephrotoxicity: Role of oxidative stress. J Lab Physicians. 2019;11(3):200-205. doi: 10.4103/JLP.JLP_136_18.5. Hoca M, Becer E, Kabadayı H, Yücecan S, Vatansever HS. The Effect of Resveratrol and Quercetin on Epithelial-Mesenchymal Transition in Pancreatic Cancer Stem Cell. Nutr Cancer. 2019; 9:1-12. doi: 10.1080/01635581.2019.1670853. 6. Marunaka Y, Marunaka R, Sun H, Yamamoto T, Kanamura N, Inui T, Taruno A. Actions of Quercetin, a Polyphenol, on Blood Pressure. Molecules. 2017;22(2). pii: E209. doi: 10.3390/molecules22020209.7. Piovezana Bossolani GD, Silva BT, Colombo Martins Perles JV, Lima MM, Vieira Frez FC, Garcia de Souza SR, Sehaber-Sierakowski CC, Bersani-Amado CA, Zanoni JN. Rheumatoid arthritis induces enteric neurodegeneration and jejunal inflammation, and quercetin promotes neuroprotective and anti-inflammatory actions. Life Sci. 2019;14:116956. doi: 10.1016/j.lfs.2019.116956.8. Ertuğ P, Olguner AA, Öğülener N, Şingirik E. Protective effect of quercetin a polyphenolic compound, on mouse corpus cavernosum. Fundamental and Clinical Pharmacology 2010; 24: 223-32. doi: 10.1111/j.1472-8206.2009.00755.x.9. Hirano M, Garone C, Quinzii CM. Co Q (10) deficiencies and MNGIE: Two treatable mitochondrial disorders. Biochim Biophys Acta. 2012; 1820:625-31. doi: 10.1016/j.bbagen.2012.01.006.10. Ognjanovic BI, Markovic SD, Pavlovic SZ, Zikic RV, Stajn AS, Saicic ZS. Combined effects of coenzyme Q10 and Vitamin E in cadmium induced alterations of antioxidant defense system in the rat heart. Environmental Toxicology and Pharmacology 2006; 22: 219-24. https://doi.org/10.1016/j.etap.2006.03.008.11. Lee BJ, Huang YC, Chen SJ, Lin PT. Coenzyme Q10 supplementation reduces oxidative stress and increase antioxidant enzyme activity in patients with coronary artery disease. Nutrition. 2012; 28: 250-5. doi: 10.1016/j.nut.2011.06.004.12. Tafazoli A. Coenzyme Q10 in breast cancer care. Future Oncol. 2017;13(11):1035-1041. doi: 10.2217/fon-2016-0547.13. Polat A, Parlakpınar H, Taşdemir S, Çolak C, Vardı N, Uçar M, Emre MH, Acet A. Protective role of aminoguanidine on gentamicin-induced acute renal failure in rats. Acta Histochemica 2006;108: 365-71. https://doi.org/10.1016/j.acthis.2006.06.00514. Cuzzocrea S, Mazzon E, Dugo L, Serraino I, Di Paola R et all. A role for superoxide in gentamicin-mediated nephropathy in rats. Eur J Pharmacol. 2002;450: 67-76. https://doi.org/10.1016/S0014-2999(02)01749-1.15. Neslihan Pinar, Oguzhan Ozcan, Esin Dogan, Gokhan Cakirca. Protective effect of dexpanthenol on gentamicin-induced nephrotoxicity in rats. Annals of Medical Research. 2019;26(9):1787-91. DOI: 10.5455/annalsmedres.2019.07.435. 16. Altintas R, Parlakpinar H, Beytur A, et al. Protective effect of dexpanthenol on ischemia-reperfusion-induced renal injury in rats. Kidney Blood Press Res 2012;36:220-30. doi: 10.1159/000343411.17. Sen H, Deniz S, Yedekci AE, et al. Effects of dexpanthenol and N-acetylcysteine pretreatment in rats before renal ischemia/reperfusion injury. Ren Fail 2014;36:1570-4. doi: 10.3109/0886022X.2014.949768.18. Rodrigo R, Bosco C. Oxidative stres and protective effects of polyphenols: Comparative studies in human and rodent kidney. A review. Comp Biochem Physiol C Toxicol Pharmacol.2006; 142: 317-27. https://doi.org/10.1016/j.cbpc.2005.11.002.19. Lee BJ, Huang YC, Chen SJ, Lin PT. Effects of Coenzyme Q10 supplementation on inflammatory markers (high-sensitivity C-reactive protein, interleukin-6, and homocysteine) in patients with coronary artery disease. Nutrition 2012;28:767-72. doi: 10.1016/j.nut.2011.11.008.20. Persson MF, Franzén S, Catrina SB, Dallner G, Hansell P, Brismar K, Palm F. Coenzyme Q10 prevents GDP-sensitive mitochondrial uncoupling, glomerular hyperfiltration and proteinuria in kidneys from db/db mice as a model of type 2 diabetes. Diabetologia 2012; 55:1535-1543. doi: 10.1007/s00125-012-2469-5.
Abstract
Purpose: We investigated the effects of Quercetin and Coenzyme Q10 antioxidant, on gentamicin-induced renal failure in rats.
Amaç: Gentamisinle böbrek yetmezliği oluşturulan sıçanlarda Quercetin ve CoQ10 nun antioksidan etkilerini araştırmak amaçlanmıştır.
Gereç ve Yöntem: Sıçanlara gentamicin (100 mg/kg/day, i.m., günde 1 kez), gentamicin (100 mg/kg/day, i.m.) + Quercetin (15 mg/kg i.p. günde 1 kez) and gentamicin (100 mg/kg/day, i.m.) + CoQ10 (8 mg/kg i.m. günde 1 kez) uygulandı.
Bulgular: Kontrol grubundaki BUN değeri 26.8 ± 0.8 (mg/100 mL); buna karşın gentamisin grubunda 117.3 ± 15.4 bulunmuştur. Böbrek histopatolojik inceleme, bu grupta akut tübüler nekrozu doğruladı. Gentamicin + Quercetin ve gentamicin + CoQ10 uygulanan sıçanlarda biyokimyasal ve histolojik parametrelerde kısmi iyileşme gözlemlenmiştir. BUN değerleri gentamisin, gentamisin + Quercetin and gentamisin + CoQ10 gruplarında sırasıyla 117.3 ± 15.4, 44.0 ± 8.3, 47.8 ± 13.8 bulunmuştur. Gentamisin ile aralarında anlamlı farklılık bulunmuştur. Kreatinin değerleri gentamisin, gentamisin + Quercetin ve gentamisin + Koenzim Q10 ile tedavi edilen gruplarda sırasıyla 4.3 ± 0.6, 0.8 ± 0.1, 1.5 ± 0.4 idi.
Sonuç: Bu sonuçlar Quercetin ve CoQ10 uygulanmasının gentamisinle nefrotoksisite oluşturulan sıçanlarda koruyucu etkisi olduğunu göstermiştir.
Keywords
Project Number
2006/BAP 31
References
- 1. Vaishnu Devi D, Viswanathan P. Sulphated polysaccharide from Sargassum myriocystum confers protection against gentamicin-induced nephrotoxicity in adult zebrafish. Environ Toxicol Pharmacol. 2019 23;72:103269. doi: 10.1016/j.etap.2019.103269.2. Abdel-Raheem IT, Abdel-Ghany AA, Mohamed GA. Protective effect of Quercetin against gentamisin-induced nephrotoxicity in rats. Biol. Pharm. Bull 2009; 32: 61-7. doi.org/10.1248/bpb.32.61.3. Rasheed HA, Al-Kuraishy HM, Al-Gareeb AI. Rosuvastatin Attenuates acute nephrotoxicity through modulation of oxidative stress in Sprague Dawley rats. J Pak Med Assoc. 2019;69(8): S98-S102. The 14th Scientific International Conference.4. Al-Kuraishy HM, Al-Gareeb AI, Al-Naimi MS. Renoprotective effect of irbesartan in a rat model of gentamicin-induced nephrotoxicity: Role of oxidative stress. J Lab Physicians. 2019;11(3):200-205. doi: 10.4103/JLP.JLP_136_18.5. Hoca M, Becer E, Kabadayı H, Yücecan S, Vatansever HS. The Effect of Resveratrol and Quercetin on Epithelial-Mesenchymal Transition in Pancreatic Cancer Stem Cell. Nutr Cancer. 2019; 9:1-12. doi: 10.1080/01635581.2019.1670853. 6. Marunaka Y, Marunaka R, Sun H, Yamamoto T, Kanamura N, Inui T, Taruno A. Actions of Quercetin, a Polyphenol, on Blood Pressure. Molecules. 2017;22(2). pii: E209. doi: 10.3390/molecules22020209.7. Piovezana Bossolani GD, Silva BT, Colombo Martins Perles JV, Lima MM, Vieira Frez FC, Garcia de Souza SR, Sehaber-Sierakowski CC, Bersani-Amado CA, Zanoni JN. Rheumatoid arthritis induces enteric neurodegeneration and jejunal inflammation, and quercetin promotes neuroprotective and anti-inflammatory actions. Life Sci. 2019;14:116956. doi: 10.1016/j.lfs.2019.116956.8. Ertuğ P, Olguner AA, Öğülener N, Şingirik E. Protective effect of quercetin a polyphenolic compound, on mouse corpus cavernosum. Fundamental and Clinical Pharmacology 2010; 24: 223-32. doi: 10.1111/j.1472-8206.2009.00755.x.9. Hirano M, Garone C, Quinzii CM. Co Q (10) deficiencies and MNGIE: Two treatable mitochondrial disorders. Biochim Biophys Acta. 2012; 1820:625-31. doi: 10.1016/j.bbagen.2012.01.006.10. Ognjanovic BI, Markovic SD, Pavlovic SZ, Zikic RV, Stajn AS, Saicic ZS. Combined effects of coenzyme Q10 and Vitamin E in cadmium induced alterations of antioxidant defense system in the rat heart. Environmental Toxicology and Pharmacology 2006; 22: 219-24. https://doi.org/10.1016/j.etap.2006.03.008.11. Lee BJ, Huang YC, Chen SJ, Lin PT. Coenzyme Q10 supplementation reduces oxidative stress and increase antioxidant enzyme activity in patients with coronary artery disease. Nutrition. 2012; 28: 250-5. doi: 10.1016/j.nut.2011.06.004.12. Tafazoli A. Coenzyme Q10 in breast cancer care. Future Oncol. 2017;13(11):1035-1041. doi: 10.2217/fon-2016-0547.13. Polat A, Parlakpınar H, Taşdemir S, Çolak C, Vardı N, Uçar M, Emre MH, Acet A. Protective role of aminoguanidine on gentamicin-induced acute renal failure in rats. Acta Histochemica 2006;108: 365-71. https://doi.org/10.1016/j.acthis.2006.06.00514. Cuzzocrea S, Mazzon E, Dugo L, Serraino I, Di Paola R et all. A role for superoxide in gentamicin-mediated nephropathy in rats. Eur J Pharmacol. 2002;450: 67-76. https://doi.org/10.1016/S0014-2999(02)01749-1.15. Neslihan Pinar, Oguzhan Ozcan, Esin Dogan, Gokhan Cakirca. Protective effect of dexpanthenol on gentamicin-induced nephrotoxicity in rats. Annals of Medical Research. 2019;26(9):1787-91. DOI: 10.5455/annalsmedres.2019.07.435. 16. Altintas R, Parlakpinar H, Beytur A, et al. Protective effect of dexpanthenol on ischemia-reperfusion-induced renal injury in rats. Kidney Blood Press Res 2012;36:220-30. doi: 10.1159/000343411.17. Sen H, Deniz S, Yedekci AE, et al. Effects of dexpanthenol and N-acetylcysteine pretreatment in rats before renal ischemia/reperfusion injury. Ren Fail 2014;36:1570-4. doi: 10.3109/0886022X.2014.949768.18. Rodrigo R, Bosco C. Oxidative stres and protective effects of polyphenols: Comparative studies in human and rodent kidney. A review. Comp Biochem Physiol C Toxicol Pharmacol.2006; 142: 317-27. https://doi.org/10.1016/j.cbpc.2005.11.002.19. Lee BJ, Huang YC, Chen SJ, Lin PT. Effects of Coenzyme Q10 supplementation on inflammatory markers (high-sensitivity C-reactive protein, interleukin-6, and homocysteine) in patients with coronary artery disease. Nutrition 2012;28:767-72. doi: 10.1016/j.nut.2011.11.008.20. Persson MF, Franzén S, Catrina SB, Dallner G, Hansell P, Brismar K, Palm F. Coenzyme Q10 prevents GDP-sensitive mitochondrial uncoupling, glomerular hyperfiltration and proteinuria in kidneys from db/db mice as a model of type 2 diabetes. Diabetologia 2012; 55:1535-1543. doi: 10.1007/s00125-012-2469-5.
Details
| Primary Language | English |
|---|---|
| Subjects | Clinical Sciences (Other) |
| Journal Section | Research |
| Authors | |
| Project Number | 2006/BAP 31 |
| Publication Date | March 31, 2020 |
| Acceptance Date | January 25, 2020 |
| Published in Issue | Year 2020 Volume: 45 Issue: 1 |
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