ISHS Acta Horticulturae 1248: XII International Conference on Grapevine Breeding and Genetics
Use of the NeoViGen96 chip to understand the defense status of cultivars and resistant genotypes of Vitis vinifera |
| Authors: | M.F. Corio-Costet, A. Bellée, M.C. Dufour, D. Merdinoglu, C. Coppin |
| Keywords: | gene expression, Vitis, hybrids, chip, mildews, markers, defense status |
| DOI: | 10.17660/ActaHortic.2019.1248.74 |
| Abstract:
The NeoViGen96 chip, a pioneering tool based on high-throughput reverse-transcription quantitative PCR of 85 defense-related genes, has been developed to assess the defense status of grapevine genotypes and correlate this with protection level (Dufour et al., 2016). It is useful for setting up alternative or complementary pest management methods using plant defense stimulators, associated or not with other pest management methods such as biological control or plant breeding. It can be used to monitor expression levels of a set of selected defense genes widely covering the various defense pathways (salicylic acid, jasmonate/ethylene-dependent signal transduction, pathogenesis-related proteins, phytoalexin production and cell wall reinforcement). It has been used under controlled conditions to assess the defenses of susceptible cultivars and/or grapevine hybrids partially or totally resistant to downy and powdery mildew. It can be used to visualize defense mechanisms in leaves of resistant genotypes having different resistance quantitative trait loci, and/or cultivars and hybrids such as 'Solaris', 'Regent', Rv4 and Rv5. It can help in understanding which metabolic pathways are important for protection against powdery and downy mildews, or other diseases, and can be used to select resistant genotypes. | |
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