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ISHS Acta Horticulturae 1083: VIII International Symposium on In Vitro Culture and Horticultural Breeding

PLANT PATHOGEN ERADICATION BY CRYOTHERAPY OF SHOOT TIPS: DEVELOPMENT, ACHIEVEMENTS AND PROSPECTIVE

Authors:   Z.-H. Cui, B.-Q. Li, W.-L. Bi, J.-W. Li, Q.-C. Wang
Keywords:   bacterium, cryotherapy, phytoplasma, plant pathogen, shoot tips, virus
DOI:   10.17660/ActaHortic.2015.1083.2
Abstract:
Plant pathogen diseases have for a long time been a great threat to the sustainable development of horticulture. Use of disease-free planting materials is pivotal for the control of plant diseases. Cryotherapy, a novel biotechnology technique developed recently based on cryopreservation techniques, can efficiently eliminate plant pathogens, such as virus, phytoplasma and bacteria. In comparison with the more traditional methods, cryotherapy of shoot tips avoids excision of small meristems and consistently produces higher frequencies of pathogen-free plants. Thus, cryotherapy of shoot tips provides an alternative method for plant pathogen eradication and can be used for preparation of plant materials for long term storage of plant germplasm.

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