ISHS Acta Horticulturae 226: International Symposium on Propagation of Ornamental Plants
MULTIELEMENT ANALYSIS OF AGAR AND GELRITE IMPURITIES INVESTIGATED BY INDUCTIVELY COUPLED PLASMA EMISSION SPECTROMETRY AS WELL AS PHYSICAL PROPERTIES OF TISSUE CULTURE MEDIA PREPARED WITH AGAR OR THE GELLAN GUM GELRITE |
| Authors: | P.A. Scherer, E. Müller, H. Lippert, G. Wolff |
| DOI: | 10.17660/ActaHortic.1988.226.91 |
| Abstract:
ICP emission spectrometry revealed that the gellan gum Gelrite (K 9A40, Kelco, USA) of bacteral origin contained more cations than the polysaccharide agar of marine origin (purified quality M 1614 of Merck, FRG). This was in congruence with the ash content given by the manufacturers: 9.5% for Gelrite and 1.8% - less 5% for different agar brands. Gelrite used at 0.20% could substantially modify a MS medium with respect to Na, Al, Sr, Ti, Cu and V, whereas the sulfatated agar changed especially the S (SO42-) and Si content of the MS medium. For agar this result cannot be generalized as agar qualities differ strongly. This was seen with regard to organic impurities measured by a simple UV absorbance test at 210 and 270 nm. Agar qualities being appr. white in colour showed the lowest UV absorbance (exception Noble agar of Difco, USA). Growth experiments by balancing the above elements did not validate the first impression that inorganic components were responsible for the observed better growth characteristics of Gelrite media (Scherer, Acta Horticult., 1987). However, physical parameters could be responsible. Determinations of diffusion, water activity and water potential are presented. | |
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