ISHS Acta Horticulturae 212: Symposium on In Vitro Problems Related to Mass Propagation of Horticultural Plants
FACTORS AFFECTING ESTABLISHMENT AND DEVELOPMENT OF MAGNOLIA HYBRIDS IN VITRO |
| Author: | Ilse E.G. Biedermann |
| DOI: | 10.17660/ActaHortic.1987.212.104 |
| Abstract:
Dormant axillary buds of various magnolia hybrids, 'Elizabeth', 'Yellow Bird', 'cultivar #149' and Magnolia stellata were cultured on Murashige-Skoog medium (MS), Anderson-Rhododendron medium (AR) and a medium containing lower concentrations of macronutrients than the other two. All media were supplemented with m-inositol, 500 mg/L; Jacquoit vitamins; benzyladenine, 5–15 mg/L; and napthaleneacetic acid, 1–5 mg/L. Cultures were maintained on a 16 hour photoperiod or total darkness at 21 C for 4 weeks. Cultures with 240 mg/L KNO3 concentration and carbohydrates of 1% at low light intensity had a greater survival rate and larger calli development. All hybrids did better on the medium containing low concentrations of macronutrients. | |
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