ISHS Acta Horticulturae 164: VI International Symposium on Virus Diseases of Ornamental Plants
DETECTION AND IDENTIFICATION OF VIROIDS AND VIRAL NUCLEIC ACIDS BY "DOT-BLOT" HYBRIDIZATION |
| Authors: | P. Palukaitis, S. Cotts, M. Zaitlin |
| DOI: | 10.17660/ActaHortic.1985.164.12 |
| Abstract:
A rapid, simple and improved extraction procedure for the detection and identification of viroids and plant viruses by dot-blot hybridization is described. The entire tissue extraction can be carried out in a single tube and can be used to detect viroids or viruses in a number of plant tissues; i.e., leaves, stems, tubers, shoots, roots and seeds. The extracts are blotted onto a nitro-cellulose membrane and incubated with 32p-labeled complementary DNA (cDNA) probes to detect and identify the infectious agent. The sensitivity of detection is comparable to that achieved with phenol-extracted nucleic acids. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
