Plant Protect. Sci., 2014, 50(4):179-183 | DOI: 10.17221/30/2014-PPS

Occurrence, isolation, and identification of Acidovorax citrulli from Melon in TurkeyOriginal Paper

Sumer HORUZ1, Raziye CETINKAYA-YILDIZ2, Mustafa MIRIK3, Yesim AYSAN1
1 Plant Protection Department, Faculty of Agriculture, Cukurova University, Adana, Turkey
2 Biological Control Research Station, Adana, Turkey
3 Plant Protection Department, Faculty of Agriculture, Namik Kemal University, Tekirdag, Turkey$2

During February and August of 2010 and 2011, disease symptoms were detected in melon (Cucumis melo cv. Surmeli) fields and commercial nurseries in Adana and Mersin provinces (Eastern Mediterranean Region, Turkey). Lesions on leaves and fruits were observed in nearly 75 and 85 acres production areas of melon in 2010 and 2011, respectively. Initial symptoms were water-soaked irregular spots, light brown to reddish spots advancing through veins on leaves, small water-soaked lesions, greasy, dark olive green colour areas on the surfaces of melon fruit followed by brown lesions, softening, and cracks. Thirty-five non-fluorescent, slow-growing, round, cream and Gram-negative bacterial isolates were isolated from symptomatic plant materials. The pathogenicity of the isolates was proved using melon seedlings and fruits. According to classical, serological, and molecular assays, the causal organism was identified as Acidovorax citrulli. To the best of our knowledge, this is the first report and occurrence of bacterial fruit blotch disease on melon as a new host in Turkey.

Keywords: Acidovorax; Cucumis melo; disease; fruit blotch; ELISA; PCR

Published: December 31, 2014  Show citation

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HORUZ S, CETINKAYA-YILDIZ R, MIRIK M, AYSAN Y. Occurrence, isolation, and identification of Acidovorax citrulli from Melon in Turkey. Plant Protect. Sci.. 2014;50(4):179-183. doi: 10.17221/30/2014-PPS.
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    References

    1. Assis S.M.P., Mariano R.L.R., Silva-Hanlin D.M.W., Duart V. (1999): Bacterial fruit blotch caused by Acidovorax avenae subsp. citrulli in melon, in the State of Rio Grande do Norte, Brazil. Fitopatologia Brasileira, 24: 191.
    2. Babadoost M., Pataky N. (2002): First report of bacterial fruit blotch of watermelon caused by Acidovorax avenae subsp. citrulli in Illinois. Plant Disease, 86: 443. Go to original source... Go to PubMed...
    3. Black M.C., Isakeit T., Barnes L.W., Kucharek T.A., Hoover R.J., Hodge N.C. (1994). First report of bacterial fruit blotch of watermelon in Texas. Plant Disease, 78: 831. Go to original source...
    4. Burdman S., Kots N., Kritzman G., Kopelowitz J. (2005): Molecular, physiological and host-range characterization of Acidovorax avenae subsp. citrulli isolates from watermelon and melon in Israel. Plant Disease, 89: 1339-1347. Go to original source... Go to PubMed...
    5. Demir G. (1996): A new bacterial disease of watermelon in Turkiye: Bacterial fruit blotch of watermelon (Acidovorax avenae subsp. citrulli (Schaad et al.) Williams et al.). Journal of Turkish Phytopathology, 25: 43-49.
    6. Evans T.A., Mulrooney R.P. (1991): First report of watermelon fruit blotch in Delaware. Plant Disease, 75: 1074. Go to original source...
    7. Fessehaie A., Walcott R.R. (2005): Biological control to protect watermelon blossoms and seed from infection by Acidovorax avenae subsp. citrulli. Phytopathology, 95: 413-419. Go to original source... Go to PubMed...
    8. Gregersen T. (1978): Rapid method for distinction of gram-negative from gram-positive bacteria. European Journal of Applied Microbiology and Biotechnology. 5: 123-127. Go to original source...
    9. Isakeit T., Black M.C., Barnes L.W., Jones J.B. (1997): First report of infection of honeydew with Acidovorax avenae subsp. citrulli. Plant Disease, 81: 694-694. Go to original source... Go to PubMed...
    10. Isakeit T., Black M.C., Barnes L.W., Jones J.B. (1998): Natural infection of citronmelon with Acidovorax avenae subsp. citrulli. Plant Disease, 82: 351-351. Go to original source... Go to PubMed...
    11. Jacobs J.L., Damicone J.P., Mccraw B.D. (1992): First report of bacterial fruit blotch of watermelon in Oklahoma. Plant Disease, 76: 1185. Go to original source...
    12. King E.O., Ward M. K., Raney D.E. (1954): To simple media for the demonstration of pyocyanin and flueorescein. Journal of Clinic Medicine, 44: 301-307.
    13. Langston Jr. D.B., Walcott R.R., Gitaitis R.D., Sanders F.H. (1999): First report of a fruit rot of pumpkin caused by Acidovorax avenae subsp. citrulli in Georgia. Plant Disease, 83: 199. Go to original source... Go to PubMed...
    14. Latin R.X., Rane K.K. (1990): Bacterial fruit blotch of watermelon in Indiana. Plant Disease, 74: 331. Go to original source...
    15. Lelliot R.A., Stead D.E. (1987): Methods for the Diagnosis of Bacterial Diseases Plants. Blackwell Scientific Publications, Oxford.
    16. Martin H.L., O'Brien R.G., Abbott D.V. (1999): First report of Acidovorax avenae subsp. citrulli as a pathogen of cucumber. Plant Disease, 83: 965. Go to original source... Go to PubMed...
    17. Medeiros F.H.V., Moraes Iale S.F., Silva Neto E.B.D., Silveira E.B., Mariano Rosa D.L.R. (2009): Management of melon bacterial blotch by plant beneficial bacteria. Phytoparasitica, 37: 453-460. Go to original source...
    18. Mengulluoglu M., Soylu S. (2012): Antibacterial activities of essential oils extracted from medicinal plants against seed-borne bacterial disease agent, Acidovorax avenae subsp. citrulli. Research in Crop. 13: 641-646.
    19. Mirik M., Aysan Y., Sahin F. (2006): Occurrence of bacterial fruit blotch of watermelon caused by Acidovorax avenae subsp. citrulli in the Eastern Mediterranean Region of Turkey. Plant Disease, 90: 829. Go to original source... Go to PubMed...
    20. Nejat N., Sijam K., Abdullah S.N.A., Vadamalai G., Dickinson M. (2009): Molecular characterization of a phytoplasma associated with Coconut Yellow Decline (CYD) in Malaysia. American Journal of Applied Sciences, 6: 1331-1340. Go to original source...
    21. O'Brien R.G., Martin H.L. (1999): Bacterial blotch of melons caused by strains of Acidovorax avenae subsp. citrulli. Australian Journal of Experimental Agriculture, 39: 479-485. Go to original source...
    22. Oliveira A. de., Santos M.H.M. dos, Silveira E.B. da., Gomes A.M.A., Mariano R. de L.R. (2006): Biocontrol of bacterial blotch of melon by seed treatment with epiphytic and endophytic bacteria. Horticultura Brasileira, 24: 373-377. Go to original source...
    23. Somodi G.C., Jones J.B., Hopkins D.L., Stall R.E., Kucharek T.A., Hodge N.C., Watterson J.C. (1991): Occurrence of a bacterial watermelon fruit blotch in Florida. Plant Disease, 75: 1053-1056. Go to original source...
    24. Sowell G. Jr. (1981): A bacterial disease causing severe damage to susceptible plant introductions of muskmelon. Plant Disease, 65: 609-610. Go to original source...
    25. Wang X., Li G., Jiang D., Huang H.-Ch. (2009): Screening of plant epiphytic yeasts for biocontrol of bacterial fruit blotch (Acidovorax avenae subsp. citrulli) of hami melon. Biological Control, 50: 164-171. Go to original source...
    26. Zhao T.C., Sun F. Z., Wang B.W., Hui W.G. (2001): Pathogen identification of Hami melon bacterial fruit blotch. Acta Phytopathologica Sinica, 31: 357-364.

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