Czech J. Genet. Plant Breed., 2012, 48(4):178-184 | DOI: 10.17221/21/2012-CJGPB

Assessment of drought tolerance of some Triticum L. species through physiological indicesOriginal Paper

Muhammad Abdul Rab Faisal SULTAN1,2, Liu HUI1,2, Lv Jin YANG1,2, Zhao Hui XIAN1,2
1 State Key Laboratory of Crop Stress Biology for Arid Areas,
2 College of Life Sciences, Northwest A & F University, Shaanxi, Yangling, China

Wheat is one of the most important crops in the world. Its yield is greatly influenced by global climate change and scarcity of water in the arid and semi-arid areas of the world. So, exploration of gene resources is of importance to wheat breeding in order to improve the crop ability of coping with abiotic stress environment. Wild relatives of wheat are rich repositories of beneficial genes that confer tolerance or resistance not only to drought but also to other environmental stresses. In the present study, the changes in leaf relative water content (RWC), free proline content, and malondialdehyde (MDA) accumulation of five wild wheat species including T. boeticum (YS-1L), T. dicoccum var. dicoccoides (YS-2L), T. araraticum (ALLT), and two cultivated varieties of T. turgidum ssp. durum (MXLK and 87341), with two well-known common wheat cultivars (SH6 and ZY1) possessing strong drought resistance and sensitiveness, respectively, as references were investigated during 3-day water stress and 2-day recovery, in order to assess the drought tolerance of these wild wheat species. The laboratory experiment was conducted under two water regimes (stress and non-stress treatments). Stress was induced to hydroponically grown two weeks old wheat seedlings with 20% PEG 6000. Stress treatment caused a much smaller decrease in the leaf RWC and rise in MDA content in YS-1L compared to the other wheat species. From the data it was obvious that YS-1L was the most drought tolerant among studied species having significantly higher proline and RWC while lower MDA content under water stress conditions. The order of water stress tolerance of these species according to the three parameters is: YS-1L > YS-2L > SH6 > 87341 > ZY1 > MXLK > ALLT. We speculate that the observed drought stress tolerance at a cellular level was associated with the ability to accumulate proline and high water level conservation.

Keywords: drought tolerance; MDA; proline; relative water content; Triticum L. species; water stress

Published: December 31, 2012  Show citation

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SULTAN MARF, HUI L, YANG LJ, XIAN ZH. Assessment of drought tolerance of some Triticum L. species through physiological indices. Czech J. Genet. Plant Breed.. 2012;48(4):178-184. doi: 10.17221/21/2012-CJGPB.
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    References

    1. Abbad H., Jaafari S.E., Bort J., Araus J.L. (2004): Comparison of flag leaf and ear photosynthesis with grain yield of durum wheat under various water conditions and genotypes. Agronomie, 24: 19-28. Go to original source...
    2. Abedi T., Pakniyat H. (2010): Antioxidant enzyme changes in response to drought stress in ten cultivars of oilseed rape (Brassica napus L.). Czech Journal of Genetics and Plant Breeding, 46: 27-34. Go to original source...
    3. Al-Ghamdi A.A. (2009): Evaluation of oxidative stress tolerance in two wheat (Triticum aestivum) cultivars in response to drought. International Journal of Agriculture and Biology, 11: 7-12.
    4. Anand A., Trick H.N., Gill B.S. (2003): Stable transgene expression and random gene silencing in wheat. Plant Biotechnology Journal, 14: 241-251. Go to original source... Go to PubMed...
    5. Bandurska H., Jozwiak W. (2010): A comparison of the effects of drought on proline accumulation and peroxidases activity in leaves of Festuca rubra L. and Lolium perenne L. Acta Societatis Botanicorum Poloniae, 79: 111-116. Go to original source...
    6. Bates L.S., Weldren R.P., Teare I.D. (1973): Rapid determination of free proline for water-stressed studies. Plant Soil, 39: 205-207. Go to original source...
    7. Bogale A., Tesfaye K., Geleto T. (2011): Morphological and physiological attributes associated to drought tolerance of Ethiopian durum wheat genotypes under water deficit condition. Journal of Biodiversity and Environmental Sciences, 1: 22-36.
    8. Bricker B. (1991): MSTATC: A micro-computer program for the design, management and analysis of agronomic research experimentation. Crop and Science Department, Michigan State University, East Lansing.
    9. Cao H.X., Sun C.X., Shao H.B., Lei X.T. (2011): Effects of low temperature and drought on the physiological and growth changes in oil palm seedlings. African Journal of Biotechnology, 10: 2630-2637. Go to original source...
    10. Delauney A.J., Verma D.P.S. (1993): Proline biosynthesis and osmoregulation in plants, The Plant Journal, 4: 215-223. Go to original source...
    11. Emmerich W.E., Hardegree S.P. (1990): Polyethylene glycol solution contact effects on seed germination. Agronomy Journal, 82: 1103-1107. Go to original source...
    12. Errabii T., Gandonou C.B., Essalmani H., Abrini J., Idaomar M., Skalisenhaji N. (2006): Growth, proline and ion accumulation in sugarcane callus cultures under drought-induced osmotic stress and its subsequent relief. African Journal of Biotechnology, 5: 1488-1493.
    13. Farooq M., Wahid A., Lee D.J., Cheema S.A., Aziz T. (2010): Comparative time course action of the foliar applied glycinebetaine, salicylic acid, nitrous oxide, brassinosteroids and spermine in improving drought resistance of rice. Journal of Agronomy and Crop Science, 196: 336-345. Go to original source...
    14. Fazeli F., Ghorbanli M., Niknam V. (2007): Effect of drought on biomass, protein content, lipid peroxidation and antioxidant enzymes in two sesame cultivars. Biologia Plantarum, 51: 98-103. Go to original source...
    15. Hoagland D.R., Snyder W.C. (1993): Nutrition of strawberry plant under controlled conditions: (a) effects of deficiencies of boron and certain other elements: (b) susceptibility to injury from sodium salts. Proceedings of American Society of Horticultural Sciences, 30: 288-294.
    16. Hodges D.M., De Lond J.M., Forney C.F., Prange R.K. (1999): Improving the thiobarbituric acid-reactive substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta, 207: 604-611. Go to original source...
    17. Johari-Pireivatlou M. (2010): Effect of soil water stress on yield and proline content of four wheat lines. African Journal of Biotechnology, 9: 036-040.
    18. Lu S., Wang Z., Niu Y., Guo Z. (2008): Antioxidant responses of radiation-induced dwarf mutants of Bermuda grass to drought stress. Journal of the American Society for Horticultural Science, 133: 360-366. Go to original source...
    19. Lu Z., Neumann P. (1998): Water-stress maize, barley and rice seedlings show species diversity in mechanisms of leaf growth inhibition. Journal of Experimental Botany, 49: 1945-1952. Go to original source...
    20. Maralian H., Ebadi A., Didar R.T., Haji-Eghrari B. (2010): Influence of water deficit stress on wheat grain yield and proline accumulation rate. African Journal of Agriculture Research, 5: 286-289.
    21. Moussa H.R., Abdel-Aziz S.M. (2008): Comparative response of drought tolerant and drought sensitive maize genotypes to water stress. Australian Journal of Crop Science, 1: 31-36.
    22. Mujeeb-Kazi A., Delgado R., Cortes A., Cano S., Rosas V., Sanchez J. (2004): Contribution of synthetic hexaploids towards wheat improvement. Annual Wheat Newsletter, 50: 79-88.
    23. Pirdashti H., Tahmasebi S., Bahmanyar S. (2009): Comparison of physiological responses among four contrast rice cultivars under drought stress conditions. World Academy of Science, Engineering and Technology, 49: 52-54.
    24. Rampino P., Pataleo S., Gerardi C., Mita G., Perrotta C. (2006): Drought stress response in wheat: physiological and molecular analysis of resistant and sensitive genotypes. Plant Cell and Environment, 29: 2143-2152. Go to original source... Go to PubMed...
    25. Rhodes D., Samaras Y. (1994): Genetic control of osmoregulation in plants. In: Stronge K. (ed.): Cellular and Molecular Physiology of Cell Volume Regulation. CRC Press, Boca Raton, 347-361. Go to original source...
    26. Sanchez-Rodriguez E., Rubio-Wilhelmi M., Cervilla L.M., Blasco B., Rios J.J., Rosales M.A., Romero L., Ruiz J.M. (2010): Genotypic differences in some physiological parameters symptomatic for oxidative stress under moderate drought in tomato plants. Plant Science, 178: 30-40. Go to original source...
    27. Smirnoff N. (1993): The role of active oxygen in the response of plants to water deficit and desiccation. New Phytologist, 125: 27-58. Go to original source... Go to PubMed...
    28. Tambussi E.A., Bartoli C.G., Beltrano J., Guimet J.J., Araus J.L. (2000): Oxidative damage to thylakoid proteins in winter stressed leaves of wheat. Physiologia Plantarum, 108: 398-404. Go to original source...
    29. Tatar O., Gevrek M.N. (2008): Influence of water stress on proline accumulation, lipid peroxidation and water content of wheat. Asian Journal of Plant Sciences, 7: 409-412. Go to original source...
    30. Valkoun J.J. (2001): Wheat pre-breeding using wild progenitors. Euphytica, 119: 17-23. Go to original source...
    31. Vendruscolo A.C.G., Schuster I., Pileggi M., Scapim C.A., Molinari H.B.C., Marur C.J., Vieira L.G.C. (2007): Stress-induced synthesis of proline confers tolerance to water deficit in transgenic wheat. Journal of Plant Physiology, 164: 1367-1376. Go to original source... Go to PubMed...
    32. Villareal R.L., Banuelos O.T., Rajaram S., MujeebKazi A. (2003): Backcross-derived, synthetic bread wheats under drought stress. Annual Wheat Newsletter, 49: 65-66.
    33. Zaharieva M., Gaulin E., Havaux M., Acevedo E., Monneveux P. (2001): Drought and heat responses in the wild wheat relative Aegilops geniculata Roth: potential interest for wheat improvement. Crop Science, 41: 1321-1329. Go to original source...
    34. Zgallai H., Steppe K., Lemeur R. (2005): Photosynthetic, physiological and biochemical responses of tomato plants to polyethylene glycol-induced water deficit. Journal of Integrative Plant Biology (formerly Acta Botanica Sinica), 47: 1470-1478. Go to original source...

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