Vet Med - Czech, 2008, 53(8):420-426 | DOI: 10.17221/1931-VETMED

The wildlife hosts of Mycobacterium avium subsp. paratuberculosis in the Czech Republic during the years 2002-2007

M. Kopecna1, I. Trcka1, J. Lamka1,2, M. Moravkova1, P. Koubek3, M. Heroldova3, V. Mrlik1, A. Kralova1, I. Pavlik1
1 Veterinary Research Institute, Brno, Czech Republic
2 Faculty of Pharmacy, Charles University, Hradec Kralove, Czech Republic
3 Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, Brno, Czech Republic

The objective of this study was to determine the wildlife hosts of Mycobacterium avium subsp. paratuberculosis (MAP) in the Czech Republic. A total of 8 796 wildlife animals were examined by culture of faecal or tissue samples during the years 2002-2007. MAP was isolated from 12 (0.5%) out of 2 296 red deer (Cervus elaphus), two (0.2%) out of 835 roe deer (Capreolus capreolus), 78 (5.7%) out of 1 381 fallow deer (Dama dama), 28 (3.2%)out of 866 mouflons (Ovis musimon), four (2.5%) out of 162 chamois (Rupicapra rupicapra) and from one (0.1%) out of 805 wild boar (Sus scrofa). MAP was not cultured from 82 badgers (Meles meles), 55 martens (Martes foina), one pine marten (Martes martes), 25 brown hares (Lepus europaeus), five rabbits (Oryctolagus cuniculus), nine European polecats (Mustela putorius), two steppe polecats (Mustela eversmannii), two American minks (Mustela vison), four raccoon dogs (Nyctereutes procyonoides) and four Eurasian otters (Lutra lutra). MAP was isolated from three (2.0%) out of 149 small terrestrial mammals: one (5.9%) out of 17 brown rats (Rattus norvegicus), one (1.7%) out of 59 common voles (Microtus arvalis) and one (2.6%) out of 39 lesser white-toothed shrews (Crocidura suaveolens). Culture examinations of 34 house mice (Mus musculus) and 2 113 pigeons (Columba livia f. domestica) were negative. All 123 in vitro growing MAP isolates from wild ruminants were of IS900 RFLP type B-C1. One mouflon infected with a MAP strain which did not grow on the tested media was after IS1311-PRA-PCR assessed as being infected with a "sheep" strain. The RFLP type of the MAP isolate from the wild boar was of the RFLP type A-C10. Although the detection of MAP in wildlife in the Czech Republic was not very high, their role as a potential risk factor for cattle should be considered.

Keywords: Johne's disease; epidemiology; cattle; non-ruminant species; IS900 PCR

Published: August 31, 2008  Show citation

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Kopecna M, Trcka I, Lamka J, Moravkova M, Koubek P, Heroldova M, et al.. The wildlife hosts of Mycobacterium avium subsp. paratuberculosis in the Czech Republic during the years 2002-2007. Vet Med-Czech. 2008;53(8):420-426. doi: 10.17221/1931-VETMED.
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    References

    1. Alvarez J., de Juan L., Briones V., Romero B., Aranaz A., Fernandez-Garayzabal J.F., Mateos A. (2005): Mycobacterium avium subspecies paratuberculosis in fallow deer and wild boar in Spain. Veterinary Record, 156, 212-213. Go to original source... Go to PubMed...
    2. Beard P.M., Henderson D., Daniels M.J., Pirie A., Buxton D., Greig A., Hutchings M.R., McKendrick I., Rhind S., Stevenson K., Sharp J.M. (1999): Evidence of paratuberculosis in fox (Vulpes vulpes) and stoat (Mustela erminea). Veterinary Record, 145, 612-613. Go to original source...
    3. Beard P.M., Daniels M.J., Henderson D., Pirie A., Rudge K., Buxton D., Rhind S., Greig A., Hutchings M.R., McKendrick I., Stevenson K., Sharp J.M. (2001): Paratuberculosis infection of non-ruminant wildlife in Scotland. Journal of Clinical Microbiology, 39, 1517-1521. Go to original source... Go to PubMed...
    4. Chiodini R.J., Van Kruiningen H.J., Merkal R.S. (1984): Ruminant paratuberculosis (Johne's disease): the current status and future prospects. Cornell Veterinarian, 74, 218-262.
    5. Corn J.L., Manning E.J., Sreevatsan S., Fischer J.R. (2005): Isolation of Mycobacterium avium subsp. paratuberculosis from free-ranging birds and mammals on livestock premises. Applied and Environmental Microbiology, 71, 6963-6967. Go to original source... Go to PubMed...
    6. Crossley B.M., Zagmutt-Vergara F.J., Fyock T.L., Whitlock R.H., Gardner I.A. (2005): Fecal shedding of Mycobacterium avium subsp. paratuberculosis by dairy cows. Veterinary Microbiology, 107, 257-263. Go to original source... Go to PubMed...
    7. Daniels J., Hutchings M.R. (2001): The response of cattle and sheep to feed contaminated with rodent faeces and the implications for disease transmission. Veterinary Journal, 162, 211-218. Go to original source... Go to PubMed...
    8. de Juan L., Alvarez J., Aranaz A., Rodriguez A., Romero B., Bezos J., Mateos A., Dominguez L. (2006): Molecular epidemiology of Types I/III strains of Mycobacterium avium subspecies paratuberculosis isolated from goats and cattle. Veterinary Microbiology, 115, 102-110. Go to original source... Go to PubMed...
    9. Deutz A., Spergser J., Wagner P., Rosengarten R., Kofer J. (2005): Mycobacterium avium subps. paratuberculosis in wild animals and cattle in Styria/Austria (in German). Berliner und Munchener Tierarztliche Wochenschrift, 118, 314-320. Go to PubMed...
    10. Elton C.S. (1924): Periodic fluctuations in the numbers of animals: their causes and effects. Journal of Experimental Biology, 2, 119-163. Go to original source...
    11. Fischer O., Matlova L., Dvorska L., Svastova P., Bartl J., Melicharek I., Weston R.T., Pavlik I. (2001): Diptera as vectors of mycobacterial infections in cattle and pigs. Medical and Veterinary Entomology, 15, 208-211. Go to original source... Go to PubMed...
    12. Fischer O.A., Matlova L., Bartl J., Dvorska L., Svastova P., duMaine R., Melicharek I., Bartos M., Pavlik I. (2003a): Earthworms (Oligochaeta, Lumbricidae) and mycobacteria. Veterinary Microbiology, 91, 325-338. Go to original source... Go to PubMed...
    13. Fischer O.A., Matlova L., Dvorska L., Svastova P., Pavlik I. (2003b): Nymphs of the oriental cockroach, Blatta orientalis as passive vectors of causative agents of avian tuberculosis and paratuberculosis. Medical and Veterinary Entomology, 17, 145-150. Go to original source... Go to PubMed...
    14. Fischer O.A., Matlova L., Dvorska L., Svastova P., Bartl J., Weston R.T., Pavlik I. (2004): Blowflies Calliphora vicina and Lucilia sericata as passive vectors of Mycobacterium avium subsp. avium, M. a. paratuberculosis and M. a. hominissuis. Medical and Veterinary Entomology, 18, 116-122. Go to original source... Go to PubMed...
    15. Fischer O.A., Matlova L., Dvorska L., Svastova P., Bartos M., Weston R.T., Kopecna M., Trcka I., Pavlik I. (2005): Potential risk of Mycobacterium avium subspecies paratuberculosis spread by syrphid flies in infected cattle farms. Medical and Veterinary Entomology, 19, 360-366. Go to original source... Go to PubMed...
    16. Fischer O.A., Matlova L., Dvorska L., Svastova P., Bartos M., Weston R.T., Pavlik I. (2006): Various stages in the life cycle of syrphid flies (Eristalis tenax; Diptera: Syrphidae) as potential mechanical vectors of pathogens causing mycobacterial infections in pig herds. Folia Microbiologica, 51, 147-153. Go to original source... Go to PubMed...
    17. Florou M., Leontides L., Kostoulas P., Billinis C., Sofia M., Kyriazakis I., Lykotrafitis F. (2007): Isolation of Mycobacterium avium subspecies paratuberculosis from non-ruminant wildlife living in the sheds and on the pastures of Greek sheep and goats. Epidemiology and Infection, 136, 644-652. Go to original source... Go to PubMed...
    18. Greig A., Stevenson K., Henderson D., Perez V., Hughes V., Pavlik I., Hines M.E., McKendrick I., Sharp J.M. (1999): Epidemiological study of paratuberculosis in wild rabbits in Scotland. Journal of Clinical Microbiology, 37, 1746-1751. Go to original source... Go to PubMed...
    19. Hejlicek K., Treml F. (1995): Comparison of pathogenesis and epizootiology signification of avian mycobacteriosis in different sorts of domestic and free living synanthropic fowl (in Czech). Veterinarni Medicina, 40, 187-194.
    20. Jessup D.A., Abbas B., Behymer D. (1981): Paratuberculosis in tule elk in California. Journal of the American Veterinary Medical Association, 179, 1252-1254. Go to PubMed...
    21. Kopecna M., Ondrus S., Literak I., Klimes J., Horvathova A., Moravkova M., Bartos M., Trcka I., Pavlik I. (2006): Detection of Mycobacterium avium subsp. paratuberculosis in two brown bears in the Central European Carpathians. Journal of Wildlife Diseases, 42, 691-695. Go to original source... Go to PubMed...
    22. Krebs C.J. (1996): Population cycles revisited. Journal of Mammalogy, 77, 8-24. Go to original source...
    23. Machackova M., Matlova L., Lamka J., Smolik J., Melicharek I., Hanzlikova M., Docekal J., Cvetnic Z., Nagy G., Lipiec M., Ocepek M., Pavlik I. (2003): Wild boar (Sus scrofa) as a possible vector of mycobacterial infections: review of literature and critical analysis of data from Central Europe between 1983 to 2001. Veterinarni Medicina, 48, 51-65. http://www.vri.cz/docs/vetmed/48-3-51.pdf Go to original source...
    24. Machackova M., Svastova P., Lamka J., Parmova I., Liska V., Smolik J., Fischer O.A., Pavlik I. (2004): Paratuberculosis in farmed and free-living wild ruminants in the Czech Republic (1999-2001). Veterinary Microbiology, 101, 225-234. Go to original source... Go to PubMed...
    25. Marco I., Ruiz M., Juste R., Garrido J.M., Lavin S. (2002): Paratuberculosis in free-ranging fallow deer in Spain. Journal of Wildlife Diseases, 38, 629-232. Go to original source... Go to PubMed...
    26. Marsh I.B., Whittington R.J. (2007): Genomic diversity in Mycobacterium avium: single nucleotide polymorphisms between the S and C strains of M. avium subsp. paratuberculosis and with M. a. avium. Molecular and Cellular Probes, 21, 66-75. Go to original source... Go to PubMed...
    27. Marsh I., Whittington R., Cousins D. (1999): PCR-restriction endonuclease analysis for identification and strain typing of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium based on polymorphisms in IS1311. Molecular and Cellular Probes, 13, 115-126. Go to original source... Go to PubMed...
    28. Moravkova M., Hlozek P., Beran V., Pavlik I., Preziuso S., Cuteri V., Bartos M. (2008): Strategy for the detection and differentiation of Mycobacterium avium species in isolates and heavily infected tissues. Research in Veterinary Science, 85, 257-264. Go to original source... Go to PubMed...
    29. Mayer H., Weiss E.H. (1986): Paratuberculosis in one mufflon in Nordbaden (in German). Tierarztliche Umschau, 41, 112-117.
    30. Nebbia P., Robino P., Ferroglio E., Rossi L., Meneguz G., Rosati S. (2000): Paratuberculosis in red deer (Cervus elaphus hippelaphus) in the Western Alps. Veterinary Research Communications, 24, 435-443. Go to original source... Go to PubMed...
    31. Pavlik I., Horvathova A., Dvorska L., Bartl J., Svastova P., Dumaine R., Rychlik I. (1999): Standardisation of restriction fragment length polymorphism analysis for Mycobacterium avium subspecies paratuberculosis. Journal of Microbiological Methods, 38, 155-167. Go to original source... Go to PubMed...
    32. Pavlik I., Bartl J., Dvorska L., Svastova P., Du Maine R., Machackova M., Ayele W.Y., Horvathova A. (2000a): Epidemiology of paratuberculosis in wild ruminants studied by restriction fragment length polymorphism in the Czech Republic during the period 1995-1998. Veterinary Microbiology, 77, 231-251. Go to original source... Go to PubMed...
    33. Pavlik I., Matlova L., Bartl J., Svastova P., Dvorska L., Whitlock R. (2000b): Parallel faecal and organ Mycobacterium avium subsp. paratuberculosis culture of different productivity types of cattle. Veterinary Microbiology, 77, 309-324. Go to original source... Go to PubMed...
    34. Raizman E.A., Wells S.J., Jordan P.A., DelGiudice G.D., Bey R.K. (2005): Mycobacterium avium subsp. paratuberculosis from free-ranging deer and rabbits surrounding Minnesota dairy herds. Canadian Journal of Veterinary Research, 69, 32-38.
    35. Riemann H., Zaman M.R., Ruppanner R., Aalund O., Jorgensen J.B., Worsaae H., Behymer D. (1979): Paratuberculosis in cattle and free-living exotic deer. Journal of the American Veterinary Medical Association, 174, 841-843. Go to PubMed...
    36. Trcka I., Lamka J., Suchy R., Kopecna M., Beran V., Moravkova M., Horvathova A., Bartos M., Parmova I., Pavlik I. (2006): Mycobacterial infections in European wild boar (Sus scrofa) in the Czech Republic during the years 2002 to 2005. Veterinarni Medicina, 51, 320-332. http://www.vri.cz/docs/vetmed/51-5-320.pdf Go to original source...

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