Czech J. Anim. Sci., 2018, 63(7):263-271 | DOI: 10.17221/103/2017-CJAS

Effect of synbiotic dietary supplementation on histological and histopathological parameters of Pectoralis major muscle of broiler chickensOriginal Paper

Joanna Bogucka*,1, David Miguel Ribeiro2, R.P. Roberto da Costa2,3, Marek Bednarczyk1
1 Laboratory of Histology, Department of Animal Biochemistry and Biotechnology, University of Science and Technology in Bydgoszcz, Bydgoszcz, Poland
2 Agricultural College of Coimbra, Polytechnic Institute of Coimbra, Coimbra, Portugal
3 CERNAS - Research Centre for Natural Resources, Environment and Society, Coimbra, Portugal

Bioactive substances may constitute an alternative to antibiotics. Probiotics are mixtures of microorganisms that enhance the effectiveness and yield of nutrient absorption by competing for the substrate against pathogens that may cause intestinal infections. Prebiotics are organic substances which include complex carbohydrates and have an influence on the growth and activity of desirable intestinal microflora. Synbiotics are a combination of both of them. The aim of this study was to analyze the impact of synbiotics added to commercial feed on Pectoralis major muscle microstructure in broiler chickens. Research material consisted of 240 one-day-old Ross 308 female chicks. Birds were divided into 2 groups, 12 broilers each (replicated 10 times). The control group (C) was fed a commercial diet, and the SYN group was fed the same diet with added synbiotic: 0.8% of prebiotic RFO (raffinose family oligosaccharides) extracted from lupin seeds and 1% of probiotic Lavipan®. Birds were slaughtered at day 42. Immediately after slaughter, samples of the Pectoralis major muscle for histological analysis were taken and frozen in liquid nitrogen. The staining procedures performed were: hematoxylin and eosin staining to evaluate fibre diameter and histopathological changes, oil red staining to determine intramuscular fat content, NADH-TR (tetrazolium reductase) activity to distinguish muscle fibre types and alkaline phosphatase reaction for counting the number of capillaries. The tendency to reduced muscle fibre diameter in chickens supplemented with synbiotics indicates an increase in muscle fibre density, which gives meat a more delicate structure. When assessing the occurrence of histopathological changes, significantly more fibre splitting was observed in the control group. Moreover, the positive effect of feed supplementation with synbiotics on muscle microvascularization could have an important practical meaning, because low physical activity of chickens during rearing may lead to ischemic muscles, increased changes in the structure of muscle fibres, and reduction of meat quality.

Keywords: poultry; bioactive substances; muscle fibre; histopathological traits

Published: July 31, 2018  Show citation

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Bogucka J, Ribeiro DM, da Costa RPR, Bednarczyk M. Effect of synbiotic dietary supplementation on histological and histopathological parameters of Pectoralis major muscle of broiler chickens. Czech J. Anim. Sci.. 2018;63(7):263-271. doi: 10.17221/103/2017-CJAS.
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    References

    1. Bianchi M., Petracci M., Cavani C. (2006): The influence of genotype, market live weight, transportation and holding conditions prior to slaughter on broiler breast meat colour. Poultry Science, 85, 123-128. Go to original source... Go to PubMed...
    2. Borisov A.B., Dedkov E.I., Carlson B.M. (2001): Interrelations of myogenic response, progressive atrophy of muscle fibers and cell death in denervated skeletal muscle. The Anatomical Record, 264, 203-218. Go to original source... Go to PubMed...
    3. Chambers J.R. (2003): Probiotics for control of Salmonella in chicken. Canadian Meat Science Association News, September 2003, 6-9.
    4. Choi Y.M., Kim B.C. (2009): Muscle fibre characteristics, myofibrillar protein isoforms and quality. Livestock Science, 122, 105-118. Go to original source...
    5. Cianciullo D. (2012): Effect of prebiotic and synbiotic injected in ovo on performance, meat quality and histopathological changes in muscle of broiler chickens. Ph.D. Thesis, Molise, Italy: University of Molise. Available from http://road.unimol.it/bitstream/2192/235/1/Tesi_D_Cianciullo.pdf.
    6. Cruz R.F., Vieira S.L., Kindlein L., Kipper M., Cemin H.S., Rauber S.M. (2017): Occurrence of white striping and wooden breast in broilers fed grower and finisher diets with increasing lysine levels. Poultry Science, 96, 501-510. Go to original source... Go to PubMed...
    7. Dankowiakowska A., Bogucka J., Szczerba A., Sobolewska A., Kozlowska I., Maiorano G., Tavaniello S., Bednarczyk M. (2014): Assessment of intramuscular fat content and cholesterol content in m. pectoralis superficialis of chicken injected in ovo with bioactive substances. In: Proc. XXVI Internat. Poultry Symposium PB WPSA "Science for Poultry Practice - Poultry Practice for Science", Kazimierz Dolny upon Vistula, Poland, 59.
    8. de Vrese M., Schrezenmeir J. (2008): Probiotics, prebiotics and synbiotics. Advances in Biochemical Engineering/ Biotechnology, 111, 1-66. Go to original source... Go to PubMed...
    9. Dinh T.T.N., Thompson L.D., Galyean M.L., Brooks J.C., Patterson K.Y., Boylan L.M. (2011): Cholesterol content and methods for cholesterol determination in meat and poultry. Comprehensive Reviews in Food Science and Food Safety, 10, 269-289. Go to original source...
    10. Dransfield E., Sosnicki A.A. (1999): Relationship between muscle growth and poultry meat quality. Poultry Science, 78, 743-746. Go to original source... Go to PubMed...
    11. Elminowska-Wenda G., Szpinda M., Klosowska D. (2005): Capillaries of pectoralis and biceps femoris muscle in turkeys. Archiv für Geflügelkunde, 69, 35-39.
    12. Fouad A.M., El-Senousey H.K. (2014): Nutritional factors affecting abdominal fat deposition in poultry: a review. Asian-Australasian Journal of Animal Sciences, 27, 1057-1068. Go to original source... Go to PubMed...
    13. Gibson G.R., Roberfroid M.B. (1995): Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. The Journal of Nutrition, 6, 1401-1412. Go to original source... Go to PubMed...
    14. Hanning I., Clement A., Owens C., Park S.H., Pendleton S., Scott E.E., Almeida G., Gonzalez Gil F., Ricket S.C. (2012): Assessment of production performance in two breeds of broilers fed probiotics as feed additives. Poultry Science, 91, 3295-3299. Go to original source... Go to PubMed...
    15. Havenaar R., Huis in't Veld J.H.J. (1992): Probiotics: a general view. In: Wood B.J.B. (ed.): The Lactic Acid Bacteria: Volume I. Elsevier Applied Science, London, UK, 151-170. Go to original source...
    16. Hocquette J.F., Gondret F., Baez E., Medale F., Jurie C., Pethick D.W. (2010): Intramuscular fat content in meatproducing animals: development, genetic and nutritional control and identification of putative markers. Animal, 4, 303-319. Go to original source... Go to PubMed...
    17. Hudlicka O. (1985): Development and adaptability of microvasculature in skeletal muscle. Journal of Experimental Biology, 115, 215-228. Go to original source... Go to PubMed...
    18. Hume H.E. (2011): Historic perspective: prebiotics, probiotics and other alternatives to antibiotics. Poultry Science, 90, 2663-2669. Go to original source... Go to PubMed...
    19. Koomkrong N., Theerawatanasirikul S., Boonkaewwan C., Jaturasitha S., Kayan A. (2015): Breed-related number and size of muscle fibres and their response to carcass quality in chickens. Italian Journal of Animal Science, 14, 638-642. Go to original source...
    20. Kral M., Angelovicova M., Mrazova L. (2012): Application of probiotics in poultry production. Scientific Papers: Animal Sciences and Biotechnologies, 45, 55-57.
    21. Kuttappan V.A., Shivaprasad H.L., Shaw D.P., Valentine B.A., Hargis B.M., Clark F.D., McKee S.R., Owens C.M. (2013): Pathological changes associated with white striping in broiler breast muscles. Poultry Science, 92, 331-338. Go to original source... Go to PubMed...
    22. Le Bihan-Duval E., Debut M., Berri C.M., Sellier N., SanteLhoutellier V., Jego Y., Beaumont C. (2008): Chicken meat quality: genetic variability and relationship with growth and muscle characteristics. BMC Genetics, 9: 53. Go to original source... Go to PubMed...
    23. MacRae V.E., Mahon M., Gilpin S., Sandercock P.A., Hunter R.R., Mitchell M.A. (2007): A comparison of breast muscle characteristics in three broiler grand-parent lines. Poultry Science, 86, 382-385. Go to original source... Go to PubMed...
    24. Maiorano G., Sobolewska A., Cianciullo D., Walasik K., Elminowska-Wenda G., Slawinska A., Tavaniello S., Zylinska J., Bardowski J., Bednarczyk M. (2012): Influence of in ovo prebiotic and synbiotic administration on meat quality of broiler chickens. Poultry Science, 91, 2963-2969. Go to original source... Go to PubMed...
    25. Maiorano G., Stadnicka K., Tavaniello S., Abiuso C., Bogucka J., Bednarczyk M. (2017): In ovo validation model to assess the efficacy of commercial prebiotics on broiler performance and oxidative stability of meat. Poultry Science, 96, 511-518. Go to original source... Go to PubMed...
    26. Petracci M., Cavani C. (2012): Muscle growth and poultry meat quality issues. Nutrients, 4, 1-12. Go to original source... Go to PubMed...
    27. Sandercock D.A., Barker Z.E., Mitchel M.A., Hocking P.M. (2009): Changes in muscle cell cation regulation and meat quality traits are associated with genetic selection for high body weight and meat yield in broiler chickens. Genetics, Selection, Evolution, 41: 8. Go to original source... Go to PubMed...
    28. Santoso V., Tanaka K., Ohtani S. (1995): Effect of dried Bacillus subtillis culture on growth, body composition, and hepatic lipogenic enzyme activity in female broiler chickens. British Journal of Nutrition, 74, 523-529. Go to original source... Go to PubMed...
    29. Scanes C.G. (2007). The global importance of poultry. Poultry Science, 86, 1057-1058. Go to original source... Go to PubMed...
    30. Sihvo H.K., Immonen K., Puolanne E. (2014): Myodegeneration with fibrosis and regeneration in the Pectoralis major muscle of broilers. Veterinary Pathology, 51, 619-623. Go to original source... Go to PubMed...
    31. Sosnicki A.A., Cassens R.G., Vimini R.J., Greaser M.L. (1991): Distribution of capillaries in normal and ischemic turkey skeletal muscle. Poultry Science, 70, 343-348. Go to original source... Go to PubMed...
    32. Taherpour K., Maravel H., Shivazad M., Adibmoradi M., Yakhchali B. (2009): Effect of dietary probiotic, prebiotic and butyric acid glycerides on performance and serum composition in broiler chickens. African Journal of Biotechnology, 8, 2329-2334.
    33. Threlfall E.J., Ward L.R., Frost J.A., Willshaw G.A. (2000): The emergence and spread of antibiotic resistance in food-borne bacteria. International Journal of Food Microbiology, 62, 1-5. Go to original source... Go to PubMed...
    34. Walasik K., Lyszczarz A., Bogucka J., Kuzniacka J., Stadnicka K., Boguslawska-Tryk M., Oler A. (2017): Histopathological changes of the pectoral muscle in pheasants (Phasinus colchicus; Phasinus c. mongolicus). Acta Scientiarum Polonorum Zootechnica, 16, 15-20. Go to original source...

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