Araştırma Makalesi
Apilarnilin'in erkek sıçanlarda Lipopolisakkarite (LPS) bağlı testis toksisitesine karşı koruyucu rolünün belirlenmesi
Öz
LPS tarafından oluşturulan endotokseminin patofizyolojik mekanizmalarının anlaşılmasında ve tedavisinde pek çok strateji geliştirilmesine rağmen, hala yoğun bakım ünitelerinin büyük bir problemidir.Bu çalışmada, endotoksik şok üzerine Apilarnilin koruyucu bir etkisi olup olmadığının, testis dokusunda histopatolojik değerlendirme ve DNA yapısındaki değişimler ortaya konularak belirlenmesi amaçlanmıştır.Çalışma için 64 adet Sprague-Dawleycinsi yetişkin erkek sıçan rastgele seçilerek 8 eşit gruba ayrılmıştır.Testis dokusunda rutin hematoksilen eozin boyaması ile histopatolojik değerlendirme yapılmış, komet testi ile DNA yapısındaki değişiklikler, 6. saatin sonunda kontrol grubuyla karşılaştırılmıştır. Histolojik değerlendirme sonucunda artan dozlarda apilarnil uygulanan gruplardan alınan testislere ait kesitlerde kontrol grubu ile benzer şekilde histolojik yapı gösterdiği gözlenirken, LPS ve artan dozlarda apilarnil uygulanan gruplara ait testis dokusunda artan dozla birlikte lümendeki sperm miktarının arttığı görülmektedir. Buna ek olarak, LPS grubu ile karşılaştırıldığında bu gruplara ait seminifer tübüllerdeki hücreler arası bağlantı yerlerinde daha az ayrılmaların olduğu görülmektedir. DNA yapısındaki değişimler değerlendirildiğinde; LPS, uygulanan grupta kuyruk DNA yüzdesinde, kuyruk uzunluğunda ve kuyruk momentinde istatistiksel olarak artışa neden olmuştur. LPS+Apilarnil'in verilmesi DNA hasarında azalmaya neden olmuştur.Apilarnil’in artan dozları DNA yüzdesi ve kuyruk uzunluğunda istatistiksel olarak azalmaya neden olmuştur. Sonuç olarak, Apilarnil artan dozlarda uygulanmasının, LPS'nin neden olduğu testiküler hasarı azaltığını ve bu etkinin de apilarnilin antioksidan kapasitesinden kaynaklandığını düşünmekteyiz.
Anahtar Kelimeler
Kaynakça
- Kaynaklar
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