Xyleborus glabratus (Coleoptera: Curculionidae: Scolytinae) is a non-native pest that transmits the causal pathogen of laurel wilt disease to plants belonging to the Lauraceae. To improve the current monitoring and survey techniques of X. glabratus, various trapping and flight behavior studies were conducted in natural areas with host species in Alachua County, Florida. Daylight flight rhythm studied at Austin Cary Memorial Forest twice in Sep 2010 using sticky traps baited with manuka lures showed that X. glabratus flies mostly between 1600 and 1800 h daylight saving time. Flight height of the beetle was determined in a trapping study using ladder-like traps. The largest number of beetles was trapped at heights of 35–100 cm above the ground. Seasonality of X. glabratus was studied in Florida from Mar 2010–Dec 2011. Three peaks of trap catches occurred during Apr 2010, Oct 2010 and Mar 2011. To find the optimal Lindgren funnel trap design for X. glabratus, a study was conducted using 4, 8, 12 and 16 funnels per trap. Funnel traps with 8, 12, 16 funnels per trap captured similar numbers of X. glabratus, but significantly more than with 4 funnels per trap. The effect of aging of manuka lures was studied at 2 different sites in Alachua County, Florida. New manuka lures trapped significantly more X. glabratus than lures aged 2, 4 and 6 wk. Trap color, whether black, white, blue, yellow, red or transparent, had no significant influence on the number of X. glabratus trapped.