Abstract
Recently, returning straw to the fields has been proved as a direct and effective method to tackle soil nutrient loss and agricultural pollution. Meanwhile, the slow decomposition of straw may harm the growth of the next crop. This study aimed to determine the effects of rumen microorganisms (RMs) on straw decomposition, bacterial microbial community structure, soil properties, and soil enzyme activity. The results showed that RMs significantly enhanced the degradation rate of straw in the soil, reaching 39.52%, which was 41.37% higher than that of the control on the 30th day after straw return. After 30 d, straw degradation showed a significant slower trend in both the control and the experimental groups. According to the soil physicochemical parameters, the application of rumen fluid expedited soil matter transformation and nutrient buildup, and increased the urease, sucrase, and cellulase activity by 10%–20%. The qualitative analysis of straw showed that the hydroxyl functional group structure of cellulose in straw was greatly damaged after the application of rumen fluid. The analysis of soil microbial community structure revealed that the addition of rumen fluid led to the proliferation of Actinobacteria with strong cellulose degradation ability, which was the main reason for the accelerated straw decomposition. Our study highlights that returning rice straw to the fields with rumen fluid inoculation can be used as an effective measure to enhance the biological value of recycled rice straw, proposing a viable solution to the problem of sluggish straw decomposition.
概要
秸秆还田已被证明是解决农田养分流失和农业污染的有效手段,但目前受制于秸秆还田后分解缓慢,导致土壤病虫害等问题。配施秸秆腐解菌剂能够显著加速秸秆的腐解,探索高效促腐菌剂具有重要研究意义。本研究以瘤胃液为腐解菌剂,探讨了秸秆还田配施瘤胃液对秸秆分解、细菌微生物群落结构、土壤养分和土壤酶活性的影响。结果表明,秸秆还田后第30天,瘤胃微生物显著提高了秸秆在土壤中的降解率,实验组秸秆降解率达到39.52%,较对照组提高了41.37%。在还田30天后,对照组和实验组的秸秆降解速率都呈现出明显降低的趋势,但与对照组相比,实验组仍然表现出良好的促腐效果。此外,配施瘤胃液促进了土壤中物质转化和养分积累,增强了脲酶、蔗糖酶和纤维素酶活性。同时,秸秆中纤维素的羟基官能团结构被瘤胃微生物严重破坏。土壤微生物群落结构分析显示,配施瘤胃液导致土壤中具有较强纤维素降解能力的放线菌相对丰度增加。本研究发现,秸秆还田并配施瘤胃液的措施可为解决秸秆还田后分解缓慢的问题提供有效手段,并可作为农业废弃物资源化的有效措施。
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References
Barnes SP, Keller J, 2004. Anaerobic rumen SBR for degradation of cellulosic material. Water Sci Technol, 50(10): 305–311. https://doi.org/10.2166/wst.2004.0664
Campanaro S, Treu L, Cattani M, et al., 2017. In vitro fermentation of key dietary compounds with rumen fluid: a genome-centric perspective. Sci Total Environ, 584–585: 683–691. https://doi.org/10.1016/j.scitotenv.2017.01.096
Chaouch FC, Bouras N, Mokrane S, et al., 2016. Streptosporangium saharense sp. nov., an actinobacterium isolated from Saharan soil. Int J Syst Evol Microbiol, 66(3): 1371–1376. https://doi.org/10.1099/ijsem.0.000890
Chen M, Ma YH, Xu Y, et al., 2013. Isolation and characterization of cellulose fibers from rice straw and its application in modified polypropylene composites. Polym Plast Technol Eng, 52(15):1566–1573. https://doi.org/10.1080/03602559.2013.824465
Chung R, Kang EY, Shin YJ, et al., 2019. Development of a consolidated anaerobic digester and microbial fuel cell to produce biomethane and electricity from cellulosic biomass using bovine rumen microorganisms. J Sustainable Bioenergy Syst, 9(2):17–28. https://doi.org/10.4236/jsbs.2019.92002
Cobellis G, Trabalza-Marinucci M, Yu ZT, 2016. Critical evaluation of essential oils as rumen modifiers in ruminant nutrition: a review. Sci Total Environ, 545–546:556–568. https://doi.org/10.1016/j.scitotenv.2015.12.103
Crecchio C, Curci M, Pellegrino A, et al., 2007. Soil microbial dynamics and genetic diversity in soil under monoculture wheat grown in different long-term management systems. Soil Biol Biochem, 39(6):1391–1400. https://doi.org/10.1016/j.soilbio.2006.12.016
Dong SS, Dou S, Lin CM, et al., 2016. Decomposition rate of corn straw in soil and its effects on soil humus composition. J Jilin Agric Univ, 38(5):579–586 (in Chinese). https://doi.org/10.13327/j.jjlau.2016.3373
El-Tarabily KA, Sivasithamparam K, 2006. Non-streptomycete actinomycetes as biocontrol agents of soil-borne fungal plant pathogens and as plant growth promoters. Soil Biol Biochem, 38(7):1505–1520. https://doi.org/10.1016/j.soilbio.2005.12.017
Gaind S, Nain L, 2007. Chemical and biological properties of wheat soil in response to paddy straw incorporation and its biodegradation by fungal inoculants. Biodegradation, 18(4):495–503. https://doi.org/10.1007/s10532-006-9082-6
Han XP, Liu HJ, Hu LY, et al., 2020. Rumen microbiota characteristics and its difference between sex of Huanhu yaks. Chin J Anim Nutr, 32(1):234–243 (in Chinese). https://doi.org/10.3969/j.issn.1006-267x.2020.01.029
He K, Zhang JB, Zeng YM, 2019. Knowledge domain and emerging trends of agricultural waste management in the field of social science: a scientometric review. Sci Total Environ, 670:236–244. https://doi.org/10.1016/j.scitotenv.2019.03.184
Hu ZH, Yu HQ, 2005. Anaerobic digestion of cattail by rumen cultures. Waste Manag, 26(11):1222–1228. https://doi.org/10.1016/j.wasman.2005.08.003
Huang F, Wei HQ, Cao JH, 2015. The degradation rate of straw returned to limestone soil and the effect on soil fertility. J Resour Ecol, 6(4):217–223. https://doi.org/10.5814/j.issn.1674-764x.2015.04.004
Jin WY, Xu XC, Gao Y, et al., 2014. Anaerobic fermentation of biogas liquid pretreated maize straw by rumen microorganisms in vitro. Bioresour Technol, 153:8–14. https://doi.org/10.1016/j.biortech.2013.10.003
Khaliq A, Matloob A, Farooq M, et al., 2011. Effect of crop residues applied isolated or in combination on the germination and seedling growth of horse purslane (Trianthema portulacastrum). Planta Daninha, 29(1): 121–128. https://doi.org/10.1590/S0100-83582011000100014
Lenka NK, Lal R, 2013. Soil aggregation and greenhouse gas flux after 15 years of wheat straw and fertilizer management in a no-till system. Soil Tillage Res, 126:78–89. https://doi.org/10.1016/j.still.2012.08.011
Li K, Zhu HR, Zhang YJ, et al., 2017. Characterization of the microbial communities in rumen fluid inoculated reactors for the biogas digestion of wheat straw. Sustainability, 9(2):243. https://doi.org/10.3390/su9020243
Li M, Wang ZN, Sun J, et al., 2021. Synergistic effect of mixed fungal pretreatment on thermogravimetric characteristics of rice straw. BioResources, 16(2):3978–3990. https://doi.org/10.15376/biores.16.23978-3990
Li PP, Zhang DD, Wang XJ, et al., 2012. Survival and performance of two cellulose-degrading microbial systems inoculated into wheat straw-amended soil. J Microbiol Biotechnol, 22(1): 126–132. https://doi.org/10.4014/jmb.1102.02021
Li S, Zhang YS, Wang YN, et al., 2011. The control effect of a multifunctional bacterial agent fit for straw amendment against wheat soil-borne diseases. Front Agric China, 5(3): 305–309. https://doi.org/10.1007/s11703-011-1116-0
Liu LR, Huang WC, Liu Y, et al., 2021. Diversity of cellulolytic microorganisms and microbial cellulases. Int Biodeterior Biodegradation, 163:105277. https://doi.org/10.1016/j.ibiod.2021.105277
Liu YL, Gu Y, Wu CS, et al., 2022. Short-term straw returning improves quality and bacteria community of black soil in Northeast China. Pol J Environ Stud, 31 (2): 1869–1883. https://doi.org/10.15244/pjoes/142480
Lu RK, 2000. Soil and Agro-Chemistry Analytical Methods. China Agricultural Science and Technology Press, Beijing, China, p.24–106 (in Chinese).
Nik Raikhan NH, 2019. Biofilm strategy for the Streptosporangium sp. survival in thermo-induction from 50 to 75°C. Int J Conserv Sci, 10(3):485–492.
Pan MZ, Gan XH, Mei CT, et al., 2017. Structural analysis and transformation of biosilica during lignocellulose fractionation of rice straw. J Mol Struct, 1127:575–582. https://doi.org/10.1016/j.molstruc.2016.08.002
Sauer S, Fischer WJ, 2012. Passive wireless irreversible humidity threshold sensor exploiting the deliquescence behavior of salts. SENSORS, 2012 IEEE, Taipei, Taiwan. IEEE, Piscataway, USA, p.1–4. https://doi.org/10.1109/ICSENS.2012.6411228
Seesatat A, Rattanasuk S, Bunnakit K, et al., 2021. Biological degradation of rice straw with thermophilic lignocellulolytic bacterial isolates and biogas production from total broth by rumen microorganisms. J Environ Chem Eng, 9(1):104499. https://doi.org/10.1016/j.jece.2020.104499
Segal L, Creely JJ, Martin AE, et al., 1959. An empirical method for estimating the degree of crystallinity of native cellulose using the X-ray diffractometer. Textile Res J, 29(10):786–794. https://doi.org/10.1177/004051755902901003
Seshadri R, Leahy SC, Attwood GT, et al., 2018. Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection. Nat Biotechnol, 36(4):359–367. https://doi.org/10.1038/nbt.4110
Sommer R, Ryan J, Masri S, et al., 2011. Effect of shallow tillage, moldboard plowing, straw management and compost addition on soil organic matter and nitrogen in a dryland barley/wheat-vetch rotation. Soil Tillage Res, 115–116:39–46. https://doi.org/10.1016/j.still.2011.06.003
Su P, Brookes PC, He Y, et al., 2016. An evaluation of a microbial inoculum in promoting organic C decomposition in a paddy soil following straw incorporation. J Soils Sediments, 16(6):1776–1786. https://doi.org/10.1007/s11368-015-1340-y
Tran PQ, Bachand SC, McIntyre PB, et al., 2021. Depthdiscrete metagenomics reveals the roles of microbes in biogeochemical cycling in the tropical freshwater Lake Tanganyika. ISME J, 15(7):1971–1986. https://doi.org/10.1038/s41396-021-00898-x
Williams AG, Withers S, Sutherland AD, 2012. The potential of bacteria isolated from ruminal contents of seaweed-eating North Ronaldsay sheep to hydrolyse seaweed components and produce methane by anaerobic digestion in vitro. Microb Biotechnol, 6(1):45–52. https://doi.org/10.1111/1751-7915.12000
Wu LP, Ma H, Zhao QL, et al., 2020. Changes in soil bacterial community and enzyme activity under five years straw returning in paddy soil. Eur J Soil Biol, 100:103215. https://doi.org/10.1016/j.ejsobi.2020.103215
Xing BS, Han YL, Wang XC, et al., 2020. Persistent action of cow rumen microorganisms in enhancing biodegradation of wheat straw by rumen fermentation. Sci Total Environ, 715:136529. https://doi.org/10.1016/j.scitotenv.2020.136529
Yan C, Yan SS, Jia TY, et al., 2019. Decomposition characteristics of rice straw returned to the soil in Northeast China. Nutr Cycl Agroecosyst, 114(3):211–224. https://doi.org/10.1007/s10705-019-09999-8
Yao ZS, Zheng XH, Wang R, et al., 2013. Nitrous oxide and methane fluxes from a rice-wheat crop rotation under wheat residue incorporation and no-tillage practices. Atmos Environ, 79:641–649. https://doi.org/10.1016/j.atmosenv.2013.07.006
Yue ZB, Yu HQ, Harada H, et al., 2007. Optimization of anaerobic acidogenesis of an aquatic plant, Canna indica L., by rumen cultures. Water Res, 41(11):2361–2370. https://doi.org/10.1016/j.watres.2007.02.031
Zuo RH, Zou F, Tian SY, et al., 2022. Differential and interactive effects of Scleroderma sp. and inorganic phosphate on nutrient uptake and seedling quality of Castanea henryi. Agronomy, 12(4):901. https://doi.org/10.3390/AGRONOMY12040901
Acknowledgments
This work was supported by the National Natural Science Foundation of China (Nos. 52160002, 21707057, and 31860595), and the Natural Science Foundation of Jiangxi Province (No. 20192BAB213018). The authors would like to thank Suzhou Deyo Bot Advanced Materials Co., Ltd. (https://www.dy-test.com) for providing support on material characterization.
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Kailun SONG: validation, formal analysis, investigation, resources, data curation, writing-original draft, writing-reviewing & editing, visualization, supervision, and project administration. Xin YIN: conceptualization, methodology, validation, formal analysis, investigation, resources, data curation, writing- reviewing & editing, and visualization. Lichun KANG: conceptualization, methodology, formal analysis, investigation, resources, data curation, and visualization. Chunhuo ZHOU: validation, formal analysis, investigation, and writing- original draft. Zicheng ZHOU, Jinhai LENG, Songwen FANG, and Guorong NI: validation, formal analysis, and investigation. All authors have read and approved the final manuscript, and therefore, have full access to all the data in the study and take responsibility for the integrity and security of the data.
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Kailun SONG, Zicheng ZHOU, Jinhai LENG, Songwen FANG, Chunhuo ZHOU, Guorong NI, Lichun KANG, and Xin YIN declare that they have no conflict of interest.
This article does not contain any studies with human or animal subjects performed by any of the authors.
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Song, K., Zhou, Z., Leng, J. et al. Effects of rumen microorganisms on the decomposition of recycled straw residue. J. Zhejiang Univ. Sci. B 24, 336–344 (2023). https://doi.org/10.1631/jzus.B2200504
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DOI: https://doi.org/10.1631/jzus.B2200504