Abstract
Ecdysis is a common phenomenon that happens throughout the life phase of the giant freshwater prawn Macrobrachium rosenbergii. It is vital to better understand the correlation between cannibalism and biochemical compound that exists during the moulting process. The objective of the present study was to determine the amino acid profile released by M. rosenbergii during the ecdysis process that promotes cannibalism. To accomplish this, changes in amino acid levels (total amino acid (TAA) and free amino acid (FAA)) of tissue muscle, exoskeleton, and sample water of culture medium from the moulting (E-stage) and non-moulting (C-stage) prawns were analysed using high-performance liquid chromatography (HPLC). Comparison study revealed that among the TAA compounds, proline and sarcosine of tissues from moulting prawn were found at the highest levels. The level of FAA from water that contains moulting prawns (E-stage) was dominated by tryptophan and proline. Significant values obtained in the present study suggested that these amino acid compounds act as a chemical cue to promote cannibalism in M. rosenbergii during ecdysis. The knowledge of compositions and compounds that were released during the moulting process should be helpful for better understanding of the mechanism and chemical cues that play roles on triggering cannibalism, and also for future dietary manipulation to improve feeding efficiencies and feeding management, which indirectly impacts productivity and profitability.
摘要
目的
确定罗氏沼虾在蜕皮过程(该过程引起同类残杀) 中释放的氨基酸组分, 并明确其中差异的氨基酸 成分.
创新点
了解罗氏沼虾蜕皮过程中生化组分的变化对于理 解同类残杀的重要意义。目前, 还没有关于同类 残杀和蜕皮过程与生化组分之间关系的研究报 道。本研究以此为切入点, 采用统计学方法探究 二者之间的关联, 并鉴定其中差异的氨基酸成 分.
方法
在人工饲养条件下, 测定了蜕皮阶段和非蜕皮阶 段的罗氏沼虾肌肉、外骨骼和养殖水体中的总氨 基酸及自由氨基酸组分, 采用双因素方差分析法 比较分析了两个阶段氨基酸组分的差异, 同时鉴 定了差异的氨基酸成分。, 在蜕皮阶段, 组织中的总氨基酸以脯氨酸和肌氨 酸的含量最高, 而水体中的自由氨基酸含量以色 氨酸和脯氨酸为主。研究结果表明, 这些氨基酸 组分在罗氏沼虾蜕皮过程中释放了促进同类残 杀的化学信号.
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Acknowledgments
Abu Seman JUNETA-NOR is grateful for the financial support received from the Ministry of Higher Education of Malaysia for the Postgraduate Scholarship Programme (MyPhD-MyBrain15). Mohamad Nor AZRA is grateful for support from the same organization to his Post-Doctoral Program in the Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Malaysia. Special thanks also goes to late Associate Professor Dr. Safiah JASMANI on her support during initial draft methodology in this work.
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Abu Seman JUNETA-NOR and Mhd IKHWANUDDIN conceived and designed the study, drafted the paper, and took responsibility for the integrity of the data and the accuracy of the data analysis. Noordiyana Mat NOORDIN, Hong-yu MA, and Norainy Mohd HUSIN developed the statistical methods and interpreted the data. Mohamad Nor AZRA revised the manuscript. All authors agree to be accountable for all aspects of the work and have read and approved the manuscript.
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Abu Seman JUNETA-NOR, Noordiyana Mat NOORDIN, Mohamad Nor AZRA, Hong-yu MA, Norainy Mohd HUSIN, and Mhd IKHWANUDDIN declare that they have no conflict of interest.
All institutional and national guidelines for the care and use of laboratory animals were followed.
Project supported by the Fundamental Research Grant Scheme from the Ministry of Higher Education, Malaysia (No. 59324)
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Juneta-Nor, A.S., Noordin, N.M., Azra, M.N. et al. Amino acid compounds released by the giant freshwater prawn Macrobrachium rosenbergii during ecdysis: a factor attracting cannibalistic behaviour. J. Zhejiang Univ. Sci. B 21, 823–834 (2020). https://doi.org/10.1631/jzus.B2000126
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DOI: https://doi.org/10.1631/jzus.B2000126