Abstract
The aim of this study was to research the changes in cytotoxicity and antibacterial properties after silver nanoparticles (AgNPs) were incorporated into the surface coating of dental alloys. AgNPs were attached to cobalt chromium alloys and pure titanium using a hydrothermal method, according to the reaction: AgNO3+NaBH4→ Ag+1/2H2+1/2B2H6+NaNO3. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate the cytotoxicity of the alloys when in contact with osteogenic precursor cells (MC3T3-E1) from mice and mesenchymal stem cells (BMSC) from rats. The antibacterial properties of dental alloys incorporating three different concentrations (10, 4, and 2 μmol/L) of AgNPs were tested on Staphylococcus aureus (SA) and Streptococcus mutans (MS). High cytotoxicity values were observed for all dental alloys that contained 0% of AgNPs (the control groups). The incorporation of AgNPs reduced cytotoxicity values. No significant difference was observed for antibacterial performance when comparing dental alloys containing AgNPs to the respective control groups. The results demonstrated that the cobalt chromium alloys and pure titanium all had cytotoxicity to MC3T3-E1 and BMSC and that the incorporation of AgNPs could reduce this cytotoxicity. The concentrations of AgNPs adopted in this study were found to have no antibacterial action against SA or MS.
中文概要
题目
纳米银颗粒粘附对牙科合金细胞毒性和抗菌性的影响
目的
评估纳米银颗粒的粘附对牙科合金的细胞毒性和抗菌性的影响,并初步探讨其作用机制。
创新点
运用MTT 法证实钴铬合金和纯钛对小鼠成骨前 体细胞(MC3T3-E1)及大鼠骨髓间充质干细胞 (BMSC)产生细胞毒性,粘附纳米银颗粒后细 胞毒性有所降低。
方法
将化学法制得的3 种浓度的纳米银颗粒分别粘附 于6 种牙科合金表面,扫描电镜观察并确认纳米 银的粘附情况。采用MTT 法检测不同浓度纳米 银颗粒的牙科合金对MC3T3-E1及BMSC的细胞 毒性。评价3 种浓度纳米银颗粒的钴铬合金和纯 钛试件浸提液对金黄色葡萄球菌和变形链球菌 的抗菌性。
结论
牙科合金对MC3T3-E1 和BMSC 细胞具有较强的 毒性,粘附纳米银颗粒后细胞毒性有所降低。3 种浓度的纳米银颗粒细胞毒性之间无显著性差 异,且这3 种浓度纳米银颗粒粘附后对牙科合金 的抗菌性无明显影响。
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Project supported by the Public Welfare Projects of Science Technology Department of Zhejiang Province (No. 2013c33139) and the Natural Science Foundation of Zhejiang Province (No. LZ14C200001), China
ORCID: Xiao-ting SHEN, http://orcid.org/0000-0002-3956-6955
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Shen, Xt., Zhang, Yz., Xiao, F. et al. Effects on cytotoxicity and antibacterial properties of the incorporations of silver nanoparticles into the surface coating of dental alloys. J. Zhejiang Univ. Sci. B 18, 615–625 (2017). https://doi.org/10.1631/jzus.B1600555
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DOI: https://doi.org/10.1631/jzus.B1600555