摘 要
目 的
研究人参皂甙Rg1 对小鼠脂肪干细胞神经样分化 的促进作用, 并初步探讨其作用机理。
创新点
证明了人参皂甙Rg1 可以通过miRNA-124 途径 促进脂肪干细胞的神经样分化。
方法
从BALB/c 小鼠腹股沟和睾丸脂肪垫处分离培养 脂肪干细胞, 利用流式细胞仪检测分离的脂肪干 细胞纯度。试验分为以下五组: 磷酸缓冲盐溶液 (PBS)组、3-异丁基-1-甲基黄嘌呤(IBMX)组、 IBMX+Rg1 低剂量组、IBMX+Rg1 中剂量组和 IBMX+Rg1 高剂量组。用细胞免疫组化方法检测 了脂肪干细胞向神经样细胞的分化效率, 用荧光 定量聚合酶链式反应(qPCR) 方法检测 miRNA-124 的表达变化, 用免疫印迹的方法检测 巢蛋白(nestin)、βIII-微管蛋白(βIII-tubulin) 及羧基端小结构域磷酸酶1(SCP1)的表达水平。
结 论
免疫组化结果显示, IBMX 可以成功诱导小鼠脂 肪干细胞向神经样细胞的分化; 免疫印迹结果显 示, Rg1 可以显著提高神经样细胞标记蛋白的表 达水平; 荧光定量PCR 结果显示, Rg1 可以促进 miRNA-124 的表达量, 进而降解神经分化抑制因 子SCP1 的表达, 促进脂肪干细胞的神经样分化 效率。
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The two authors contributed equally to this work
Project supported by the Zhejiang Provincial Natural Science Foundation of China (No. LQ14C180001) and the Fundamental Research Funds for the Central Universities (No. 2014QNA6026), China
ORCID: Fu-shan SHI, http://orcid.org/0000-0002-5250-8493
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Dong, J., Zhu, G., Wang, Tc. et al. Ginsenoside Rg1 promotes neural differentiation of mouse adipose-derived stem cells via the miRNA-124 signaling pathway. J. Zhejiang Univ. Sci. B 18, 445–448 (2017). https://doi.org/10.1631/jzus.B1600355
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DOI: https://doi.org/10.1631/jzus.B1600355