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Additive effects due to biochar and endophyte application enable soybean to enhance nutrient uptake and modulate nutritional parameters

生物炭和内生菌促进大豆增加养分吸收和调节营 养参数的叠加作用

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Abstract

We studied the effects of hardwood-derived biochar (BC) and the phytohormone-producing endophyte Galactomyces geotrichum WLL1 in soybean (Glycine max (L.) Merr.) with respect to basic, macro- and micronutrient uptakes and assimilations, and their subsequent effects on the regulation of functional amino acids, isoflavones, fatty acid composition, total sugar contents, total phenolic contents, and 1,1-diphenyl-2-picrylhydrazyl (DPPH)-scavenging activity. The assimilation of basic nutrients such as nitrogen was up-regulated, leaving carbon, oxygen, and hydrogen unaffected in BC+G. geotrichum-treated soybean plants. In comparison, the uptakes of macro- and micronutrients fluctuated in the individual or co-application of BC and G. geotrichum in soybean plant organs and rhizospheric substrate. Moreover, the same attribute was recorded for the regulation of functional amino acids, isoflavones, fatty acid composition, total sugar contents, total phenolic contents, and DPPH-scavenging activity. Collectively, these results showed that BC+G. geotrichum-treated soybean yielded better results than did the plants treated with individual applications. It was concluded that BC is an additional nutriment source and that the G. geotrichum acts as a plant biostimulating source and the effects of both are additive towards plant growth promotion. Strategies involving the incorporation of BC and endophytic symbiosis may help achieve eco-friendly agricultural production, thus reducing the excessive use of chemical agents.

摘要

目的

探讨提高大豆作物品质的方法。

创新点

研究了生物炭和植物内生菌联合使用对大豆营养 吸收和营养品质的叠加作用。

方法

采用硬木生物炭和半乳糖霉菌(Galactomyces geotrichum WLL1)对大豆进行处理,按照处理 方式的不同分成四组,包括对照组(无处理)、 G. geotrichum 处理组、生物炭处理组和生物炭与 G. geotrichum 联合处理组。通过对比研究生物炭和内生菌对大豆宏量营养素和微量营养素的吸 收和同化的作用,并观察其对功能性氨基酸、异 黄酮、脂肪酸组成、总糖含量、总酚含量和1,1- 二苯基苦基苯肼(DPPH)自由基清除能力的影 响。

结论

研究结果发现生物炭和内生菌单独或联合处理均 能增加大豆养分的吸收,促进功能性氨基酸的合 成,并提升大豆营养品质。同时,生物炭是一种 额外的营养源,而内生菌能产生生物刺激效应, 两者联合使用具有叠加作用,比单独使用更加有 效。

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Authors and Affiliations

  1. School of Applied Biosciences, Kyungpook National University, Daegu, 702-701, Korea

    Muhammad Waqas, Yoon-Ha Kim, Abdul Latif Khan, Raheem Shahzad, Sajjad Asaf, Sang-Mo Kang, Muhammad Aaqil Khan & In-Jung Lee

  2. Department of Agriculture, Abdul Wali Khan University Mardan, Mardan, 23200, Pakistan

    Muhammad Waqas

  3. Division of Plant Sciences, University of Missouri, Columbia, MO, 65211, USA

    Yoon-Ha Kim

  4. UoN Chair of Oman’s Medicinal Plants & Marine Natural Products, University of Nizwa, Nizwa, 616, Oman

    Abdul Latif Khan

  5. Department of Botany, Abdul Wali Khan University Mardan, Mardan, 23200, Pakistan

    Muhammad Hamayun

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  1. Muhammad Waqas
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Correspondence to In-Jung Lee.

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The two authors contributed equally to this work

Project supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Agriculture, Food and Rural Affairs Research Center Support Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (716001-7)

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Additive effects due to biochar and endophyte application enable soybean to enhance nutrient uptake and modulate nutritional parameters

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Waqas, M., Kim, YH., Khan, A.L. et al. Additive effects due to biochar and endophyte application enable soybean to enhance nutrient uptake and modulate nutritional parameters. J. Zhejiang Univ. Sci. B 18, 109–124 (2017). https://doi.org/10.1631/jzus.B1500262

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