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ST13, a proliferation regulator, inhibits growth and migration of colorectal cancer cell lines

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Journal of Zhejiang University SCIENCE B Aims and scope Submit manuscript

Abstract

Background and objective

ST13, is the gene encoding the HSP70 interacting protein (HIP). Previous research has shown that ST13 mRNA and protein levels are down-regulated in colorectal cancer (CRC) tissues compared with adjacent normal tissues. This study aims at the role of ST13 in the proliferation and migration of CRC cells.

Methods

The transcript level of ST13 in different CRC cell lines was evaluated by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). ST13-overexpressed and ST13-knockdown CRC cells were constructed respectively by lentiviral transduction, followed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay, plate colony formation, cell-cycle analysis, and migration assays to evaluate the influence of ST13 on proliferation and migration in vitro. Moreover, a mouse xenograft study was performed to test in vivo tumorigenicity of ST13-knockdown CRC cells.

Results

Lentivirus-mediated overexpression of ST13 in CRC cells inhibited cell proliferation, colony formation, and cell migration in vitro. In contrast, down-regulation of ST13 by lentiviral-based short hairpin RNA (shRNA) interference in CRC cells significantly increased cell proliferation and cloning efficiency in vitro. In addition, down-regulation of ST13 expression significantly increased the tumorigenicity of CRC cells in vivo.

Conclusions

ST13 gene is a proliferation regulator that inhibits tumor growth in CRC and may affect cell migration.

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Authors and Affiliations

  1. Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education, Key Laboratory of Molecular Biology in Medical Sciences, Zhejiang Province, China), the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China

    Rui Bai, Zhong Shi, Jia-wei Zhang, Dan Li & Shu Zheng

  2. Department of Gastroenterology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China

    Yong-liang Zhu

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  1. Rui Bai
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  2. Zhong Shi
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  3. Jia-wei Zhang
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  4. Dan Li
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  5. Yong-liang Zhu
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  6. Shu Zheng
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Correspondence to Shu Zheng.

Additional information

Project supported by the National Natural Science Foundation of China (Nos. 30973382 and 81101477), the National High-Tech R&D Program (863) of China (No. 2012AA02A506), and the Zhejiang Provincial International Scientific Technology Collaboration Key Project (No. 2009C14010), China

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Bai, R., Shi, Z., Zhang, Jw. et al. ST13, a proliferation regulator, inhibits growth and migration of colorectal cancer cell lines. J. Zhejiang Univ. Sci. B 13, 884–893 (2012). https://doi.org/10.1631/jzus.B1200037

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  • DOI: https://doi.org/10.1631/jzus.B1200037

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