Skip to main content
SpringerLink
Log in

HLA polymorphism of the Zhuang population reflects the common HLA characteristics among Zhuang-Dong language-speaking populations

  • Published:
Journal of Zhejiang University SCIENCE B Aims and scope Submit manuscript

Abstract

A study of the human leukocyte antigen (HLA) genetic characteristics in the Zhuang, the largest ethnic population in China, would provide insight into Zhuang history and give a useful tool for disease associations, transplantation, and anthropology. In the present study, we report the comprehensive HLA-A, HLA-B, HLA-C, and HLA-DRB1 alleles and haplotypes in the Zhuang population of southern China for the first time. A total of 13 HLA-A, 24 HLA-B, 22 HLA-C, and 18 HLA-DRB1 were identified in 104 Zhuang individuals. The frequencies of HLA-A*11:01, A*02:07, A*24:02, A*02:03, and A*33:03 on A loci, B*15:02, B*58:01, B*46:01, and B*13:01 on B loci, C*03:04, C*08:01, C*01:02, C*03:02, and C*07:02 on C loci, and DRB1*15:01, DRB1*16:02, DRB1*14:01, DRB1*15:02, and DRB1*03:01 on the DRB1 loci were S>10%. The A*33:03-C*03:02-B*58:01-DRB1*03:01 and A*02:07-C*01:02-B*46:01-DRB1*14:01 haplotypes were predominant in the Zhuang. The phylogenetic tree, as well as the analysis of haplotypes, suggested that the Zhuang are genetically similar to southern Chinese populations, especially the Zhuang-Dong language-speaking populations, such as the Bouyei, Dai, and Maonan. Even though the Zhuang and southern Chinese populations shared common alleles and haplotypes, the Zhuang has maintained its unique genetic characteristics.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bugawan, T.L., Mack, S.J., Stoneking, M., Saha, M., Beck, H.P., Erlich, H.A., 1999. HLA class I allele distributions in six Pacific/Asian populations: evidence of selection at the HLA-A locus. Tissue Antigens, 53(4):311–319. [doi:10.1034/j.1399-0039.1999.530401.x]

    Article  PubMed  CAS  Google Scholar 

  • Cao, K., Moormann, A.M., Lyke, K.E., Masaberg, C., Sumba, O.P., Doumbo, O.K., Koech, D., Lancaster, A., Nelson, M., Meyer, D., et al., 2004. Differentiation between African populations is evidenced by the diversity of alleles and haplotypes of HLA class I loci. Tissue Antigens, 63(4):293–325. [doi:10.1111/j.0001-2815.2004.00192.x]

    Article  PubMed  CAS  Google Scholar 

  • Chandanayingyong, D., 1997. Thai Black, Thai Dai Lue, Thai Normal. In: Gjertson, D.W., Terasaki, P.I. (Eds.), HLA 1998. American Society for Histocompatibility and Immunogenetics, Lenexa, p.308–313.

    Google Scholar 

  • Chen, S., Hong, W., Shao, H., Fu, Y., Liu, X., Chen, D., Xu, A., 2006. Allelic distribution of HLA class I genes in the Tibetan ethnic population of China. Int. J. Immunogenet., 33(6):439–445. [doi:10.1111/j.1744-313X.2006.00637.x]

    Article  PubMed  CAS  Google Scholar 

  • Chen, S., Ren, X., Liu, Y., Hu, Q., Hong, W., Xu, A., 2007. Human leukocyte antigen class I polymorphism in Miao, Bouyei, and Shui ethnic minorities of Guizhou, China. Hum. Immunol., 68(11):928–933. [doi:10.1016/j.humimm.2007.09.006]

    Article  PubMed  CAS  Google Scholar 

  • Deng, Y.J., Yang, G., Wu, D.Y., Hu, S.N., Li, S.B., Zhu, B.F., Zhu, J., Liu, Y., 2005. Study on the HLA-DRB1 polymorphism determined by high-resolution PCR-SBT in a population of Beijing region. J. Forensic Med., 21(4):280–285 (in Chinese).

    CAS  Google Scholar 

  • Ewens, W.J., 1972. The sampling theory of selectively neutral alleles. Theor. Popul. Biol., 3(1):87–112. [doi:10.1016/0040-5809(72)90035-4]

    Article  PubMed  CAS  Google Scholar 

  • Gendzekhadze, K., Norman, P.J., Abi-Rached, L., Graef, T., Moesta, A.K., Layrisse, Z., Parham, P., 2009. Co-evolution of KIR2DL3 with HLA-C in a human population retaining minimal essential diversity of KIR and HLA class I ligands. PNAS, 106(44):18692–18697. [doi:10.1073/pnas.0906051106]

    Article  PubMed  CAS  Google Scholar 

  • Guo, D.L., Dong, J.Z., 2000. Summarization of Chinese Nationalities (Zhonghua Mingzu Zhishi Tonglan). Yunnan Education Press, Kunming, p.98–100 (in Chinese).

    Google Scholar 

  • Guo, S.W., Thompson, E.A., 1992. Performing the exact test of Hardy-Weinberg proportion for multiple alleles. Biometrics, 48(2):361–372. [doi:10.2307/2532296]

    Article  PubMed  CAS  Google Scholar 

  • Hardy, S., Siauw, P.A., Scotland, R., Scotland, R., Gu, W.T., Fajatin, M., Luman, S., Shu, M., Song, J., Fueduska, N., Urban, V., 1997. Chinese Hong Kong Normal. In: Gjertson, D.W., Terasaki, P.I. (Eds.), HLA 1997. UCLA Tissue Typing Laboratory, Los Angeles, p.261.

    Google Scholar 

  • Hoa, B.K., Hang, N.T., Kashiwase, K., Ohashi, J., Lien, L.T., Horie, T., Shojima, J., Hijikata, M., Sakurada, S., Satake, M., et al., 2008. HLA-A, -B, -C, -DRB1 and -DQB1 alleles and haplotypes in the Kinh population in Vietnam. Tissue Antigens, 71(2):127–134. [doi:10.1111/j.1399-0039.2007.00982.x]

    Article  PubMed  CAS  Google Scholar 

  • Hong, W., Chen, S., Shao, H., Fu, Y., Hu, Z., Xu, A., 2007. HLA class I polymorphism in Mongolian and Hui ethnic groups from Northern China. Hum. Immunol., 68(5):439–448. [doi:10.1016/j.humimm.2007.01.020]

    Article  PubMed  CAS  Google Scholar 

  • Imanishi, T., Akaza, T., Kimura, A., Tokunaga, K., Gojobori, T., 1992. Allele and Haplotype Frequencies for HLA and Complement Loci in Various Ethnic Groups. In: Tsuji, K., Aizawa, M., Sasazuki, T. (Eds.), HLA 1991. Proceedings of the Eleventh International Histocompatibility Workshop and Conference. Oxford University Press, Oxford, p.1065–1220.

    Google Scholar 

  • Jia, Z.J., Fu, Y.G., Pan, D.J., Liu, Z.H., Chen, W.M., Lin, J.H., Chen, R.X., Li, A.S., Zhu, Y.F., Zhou, D.M., et al., 2002. Determination of HLA-DRB1 gene polymorphism by PCR-SBT in Lahu ethnic group of Yunnan, China. Hereditas, 24(2):131–136 (in Chinese).

    PubMed  CAS  Google Scholar 

  • Lai, S.P., Ren, H.M., Hu, H.T., Li, S.B., Lai, J.H., Yan, C.X., Zhang, H.B., Zhao, J.H., 1999. HLA-DRB alleles polymorphism in Han, Hui, Uygur and Tibetan populations in Northwestern China. Acta Genet. Sin., 26(5):447–457 (in Chinese).

    PubMed  CAS  Google Scholar 

  • Lancaster, A., Nelson, M.P., Meyer, D., Thomson, G., Single, R.M., 2003. PyPop: a software framework for population genomics: analyzing large-scale multi-locus genotype data. Pac. Symp. Biocomput., 2003:514–525.

    Google Scholar 

  • Lancaster, A.K., Single, R.M., Solberg, O.D., Nelson, M.P., Thomson, G., 2007. PyPop update—a software pipeline for large-scale multilocus population genomics. Tissue Antigens, 69(Suppl. 1):192–197. [doi:10.1111/j.1399-0039.2006.00769.x]

    Article  PubMed  CAS  Google Scholar 

  • Liu, Y., Liu, Z., Fu, Y., Jia, Z., Chen, S., Xu, A., 2006. Polymorphism of HLA class II genes in Miao and Yao nationalities of Southwest China. Tissue Antigens, 67(2):157–159. [doi:10.1111/j.1399-0039.2006.00510.x]

    Article  PubMed  CAS  Google Scholar 

  • Marsh, S.G., Albert, E.D., Bodmer, W.F., Bontrop, R.E., Dupont, B., Erlich, H.A., Fernandez-Vina, M., Geraghty, D.E., Holdsworth, R., Hurley, C.K., et al., 2010. Nomenclature for factors of the HLA system, 2010. Tissue Antigens, 75(4):291–455. [doi:10.1111/j.1399-0039.2010.01466.x]

    Article  PubMed  CAS  Google Scholar 

  • Middleton, D., Hawkins, B.R., Williams, F., Meenagh, A., Moscoso, J., Zamora, J., Arnaiz-Villena, A., 2004. HLA class I allele distribution of a Hong Kong Chinese population based on high-resolution PCR-SSOP typing. Tissue Antigens, 63(6):555–561. [doi:10.1111/j.0001-2815.2004.00234.x]

    Article  PubMed  CAS  Google Scholar 

  • Ogata, S., Shi, L., Matsushita, M., Yu, L., Huang, X.Q., Shi, L., Sun, H., Ohashi, J., Muramatsu, M., Tokunaga, K., et al., 2007. Polymorphisms of human leucocyte antigen genes in Maonan people in China. Tissue Antigens, 69(2):154–160. [doi:10.1111/j.1399-0039.2006.00698.x]

    Article  PubMed  CAS  Google Scholar 

  • Shi, H., Dong, Y.L., Wen, B., Xiao, C.J., Underhill, P.A., Shen, P.D., Chakraborty, R., Jin, L., Su, B., 2005. Y-chromosome evidence of southern origin of the East Asian-specific haplogroup O3-M122. Am. J. Hum. Genet., 77(3):408–419. [doi:10.1086/444436]

    Article  PubMed  CAS  Google Scholar 

  • Shi, L., Xu, S.B., Ohashi, J., Sun, H., Yu, J.K., Huang, X.Q., Tao, Y.F., Yu, L., Horai, S., Chu, J.Y., et al., 2006. HLA-A, HLA-B, and HLA-DRB1 alleles and haplotypes in Naxi and Han populations in southwestern China (Yunnan province). Tissue Antigens, 67(1):38–44. [doi:10.1111/j.1399-0039.2005.00526.x]

    Article  PubMed  CAS  Google Scholar 

  • Shi, L., Ogata, S., Yu, J.K., Ohashi, J., Yu, L., Shi, L., Sun, H., Lin, K., Huang, X.Q., Matsushita, M., et al., 2008. Distribution of HLA alleles and haplotypes in Jinuo and Wa populations in Southwest China. Hum. Immunol., 69(1):58–65. [doi:10.1016/j.humimm.2007.11.007]

    Article  PubMed  CAS  Google Scholar 

  • Shi, L., Yao, Y.F., Shi, L., Matsushita, M., Yu, L., Lin, Q.K., Tao, Y.F., Oka, T., Chu, J.Y., Tokunaga, K., 2010a. HLA alleles and haplotypes distribution in Dai population in Yunnan province, Southwest China. Tissue Antigens, 75(2):159–165. [doi:10.1111/j.1399-0039.2009.01407.x]

    Article  PubMed  CAS  Google Scholar 

  • Shi, L., Shi, L., Yao, Y.F., Matsushita, M., Yu, L., Huang, X.Q., Yi, W., Oka, T., Tokunaga, K., Chu, J.Y., 2010b. Genetic link among Hani, Bulang and other Southeast Asian populations: evidence from HLA-A, -B, -C, -DRB1 genes and haplotypes distribution. Int. J. Immunogenet., 37(6):467–475. [doi:10.1111/j.1744-313X.2010.00949.x]

    Article  PubMed  CAS  Google Scholar 

  • Stephens, H.A., Chandanayingyong, D., Kunachiwa, W., Sirikong, M., Longta, K., Maneemaroj, R., Wongkuttiya, D., Sittisombut, N., Rungruang, E., 2000. A comparison of molecular HLA-DR and DQ allele profiles forming DR51-, DR52-, and DR53-related haplotypes in five ethnic Thai populations from mainland Southeast Asia. Hum. Immunol., 61(10):1039–1047. [doi:10.1016/S0198-8859(00)00172-5]

    Article  PubMed  CAS  Google Scholar 

  • Sun, X., Sun, Y., Gao, X., 1997. Diversity of HLA Class I Genes in Northern and Southern Chinese. In: Charron, D. (Ed.), HLA: Genetic diversity of HLA. Functional and Medical Implication. EDK, Paris, p.155–157.

    Google Scholar 

  • Tamura, K., Dudley, J., Nei, M., Kumar, S., 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol., 24(8):1596–1599. [doi:10.1093/molbev/msm092]

    Article  PubMed  CAS  Google Scholar 

  • Tian, C., Kosoy, R., Lee, A., Ransom, M., Belmont, J.W., Gregersen, P.K., Seldin, M.F., 2008. Analysis of East Asia genetic substructure using genome-wide SNP arrays. PLoS ONE, 3(12):e3862. [doi:10.1371/journal.pone.0003862]

    Article  PubMed  Google Scholar 

  • Tokunaga, K., Imanishi, T., Takahashi, K., Juji, T., 1996. On the Origin and Dispersal of East Asian Populations as Viewed from HLA Haplotypes. In: Akazawa, T., Szathmary, E.J. (Eds.), Prehistoric Mongoloid Dispersals. Oxford University, Oxford, p.187–197.

    Google Scholar 

  • Tokunaga, K., Ishikawa, Y., Ogawa, A., Wang, H., Mitsunaga, S., Moriyama, S., Lin, L., Bannai, M., Watanabe, Y., Kashiwase, K., et al., 1997. Sequence-based association analysis of HLA class I and II alleles in Japanese supports conservation of common haplotypes. Immunogenetics, 46(3):199–205. [doi:10.1007/s002510050262]

    Article  PubMed  CAS  Google Scholar 

  • Trachtenberg, E., Vinson, M., Hayes, E., Hsu, Y.M., Houtchens, K., Erlich, H., Klitz, W., Hsia, Y., Hollenbach, J., 2007. HLA class I (A, B, C) and class II (DRB1, DQA1, DQB1, DPB1) alleles and haplotypes in the Han from southern China. Tissue Antigens, 70(6):455–463. [doi:10.1111/j.1399-0039.2007.00932.x]

    Article  PubMed  CAS  Google Scholar 

  • Watterson, G.A., 1978. The homozygosity test of neutrality. Genetics, 88(2):405–417.

    PubMed  CAS  Google Scholar 

  • Wen, S.H., Lai, M.J., Yang, K.L., 2008. Human leukocyte antigen-A, -B, and -DRB1 haplotypes of cord blood units in the Tzu Chi Taiwan Cord Blood Bank. Hum. Immunol., 69(7):430–436. [doi:10.1016/j.humimm.2008.05.012]

    Article  PubMed  CAS  Google Scholar 

  • Yang, G., Deng, Y.J., Hu, S.N., Wu, D.Y., Li, S.B., Zhu, J., Zhu, B.F., Liu, Y., 2006. HLA-A, -B, and -DRB1 polymorphism defined by sequence-based typing of the Han population in Northern China. Tissue Antigens, 67(2):146–152. [doi:10.1111/j.1399-0039.2006.00529.x]

    Article  PubMed  CAS  Google Scholar 

  • Yao, Y., Shi, L., Shi, L., Matsushita, M., Yu, L., Lin, K., Tao, Y., Huang, X., Yi, W., Oka, T., et al., 2009. Distribution of HLA-A, -B, -Cw, and -DRB1 alleles and haplotypes in an isolated Han population in Southwest China. Tissue Antigens, 73(6):561–568. [doi:10.1111/j.1399-0039.2009.01237.x]

    Article  PubMed  CAS  Google Scholar 

  • Yao, Y.G., Nie, L., Harpending, H., Fu, Y.X., Yuan, Z.G., Zhang, Y.P., 2002. Genetic relationship of Chinese ethnic populations revealed by mtDNA sequence diversity. Am. J. Phys. Anthropol., 118(1):63–76. [doi:10.1002/ajpa.10052]

    Article  PubMed  Google Scholar 

  • You, Z., 1994. History of Yunnan Nationalities. Yunnan University Press, Kunming, p.1–11 (in Chinese).

    Google Scholar 

  • Yu, H.L., Dai, G.Y., Xia, Y.Z., 2002. Investigation of the polymorphism of HLA-DRB1 alleles in minority and Han nationality in Inner Mongolia. Acta Acad. Med. Nei Mongol., 24:226–228 (in Chinese).

    Google Scholar 

  • Zhang, H.B., Gao, F., Kang, L.L., Li, S.B., 2005. Determination of HLA-A, B, and DRB1 gene polymorphism in Monba ethnic group of Xizang Autonomous Region, China. Chin. J. Med. Genet., 22(3):344–346 (in Chinese).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, 650118, China

    Li Shi, Xiao-qin Huang, Lei Shi, Yu-fen Tao, Yu-feng Yao, Liang Yu, Ke-qin Lin, Wen Yi, Hao Sun & Jia-you Chu

  2. Department of Human Genetics, Graduate School of Medicine, University of Tokyo, Tokyo, 113-0033, Japan

    Katsushi Tokunaga

Authors
  1. Li Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiao-qin Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lei Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yu-fen Tao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yu-feng Yao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Liang Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ke-qin Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Wen Yi
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Hao Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Katsushi Tokunaga
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Jia-you Chu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Katsushi Tokunaga or Jia-you Chu.

Additional information

The two authors contributed equally to this work

Project supported by the Yunnan Provincial Science and Technology Department (No. 2008CC021), the National Natural Science Foundation of China (No. 30900798), and the CAMS-JSPS China-Japan Medical Exchange Program

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shi, L., Huang, Xq., Shi, L. et al. HLA polymorphism of the Zhuang population reflects the common HLA characteristics among Zhuang-Dong language-speaking populations. J. Zhejiang Univ. Sci. B 12, 428–435 (2011). https://doi.org/10.1631/jzus.B1000285

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1631/jzus.B1000285

Key words

CLC number

Search

Navigation