Abstract
Brucellosis, a zoonosis caused by four species of brucella, has a high morbidity. Brucella melitensis is the main causative agent of brucellosis in both human and small ruminants. As an alternative to conventional antibiotics, medicinal plants are valuable resources for new agents against antibiotic-resistant strains. The aim of this study was to investigate the usage of native plants for brucellosis treatment. For this purpose, the anti-brucella activities of ethanolic and methanolic extracts of Salvia sclarea, Oliveria decumbens, Ferulago angulata, Vitex pseudo-negundo, Teucrium polium, Plantago ovata, Cordia myxa, and Crocus sativus were assessed. The activity against a resistant Br. melitensis strain was determined by disc diffusion method at various concentrations from 50–400 mg/ml. Antibiotic discs were also used as a control. Among the evaluated herbs, six plant (Salvia sclarea, Oliveria decumbens, Ferulago angulata, Vitex pseudo-negundo, Teucrium polium, and Crocus sativus) showed anti-brucella activity. Oliveria decumbens was chosen as the most effective plant for further studies. A tested isolate exhibited resistance to tetracycline, nafcillin, oxacillin, methicillin, and colistin. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values for Oliveria decumbens against resistant Br. melitensis were the same (5 mg/ml), and for gentamicin they were both 2 mg/ml. Time-kill kinetics for a methanolic extract of Oliveria decumbens was 7 h whereas for an ethanolic extract it was 28 h. Also, Oliveria decumbens extracts showed a synergistic effect in combination with doxycycline and tetracycline. In general, the similar values of MIC and MBC for Oliveria decumbens suggest that these extracts could act as bactericidal agents against Br. melitensis. In addition to Oliveria decumbens, Crocus sativus and Salvia sclarea also had good anti-brucella activity and these should be considered for further study.
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References
Aboaba, O.O., Smith, S.I., Olude, F.O., 2006. Antibacterial effect of edible plant extract on Escherichia coli O157:H7. Pakistan Journal of Nutrition, 5(4):325–327. [doi:10. 3923/pjn.2006.325.327]
Burt, A.S., Reinders, R.D., 2003. Antibacterial activity of selected plant essential oils against Escherichia coli O157: H7. Letters in Applied Microbiology, 36(3):162–167. [doi:10.1046/j.1472-765X.2003.01285.x]
Cermelli, C., Fabio, A., Fabio, G., Quaglio, P., 2008. Effect of Eucalyptus essential oil on respiratory bacteria and viruses. Current Microbiology, 56(1):89–92. [doi:10.1007/s00284-007-9045-0]
Forbes, B.A., Sahm, D.F., Weissfeld, A.S., 1998. Baily and Scott’s Diagnostic Microbiology, 10th Ed. Mosby Inc., St. Louis, USA, p.250–272.
Grilló, M.J., de Miguel, M.J., Moñoz, P.M., Marín, C.M., Ariza, J., Blasco, J.M., 2006. Efficacy of several antibiotic combinations against Brucella melitensis Rev1 experimental infection in BALB/c mice. Journal of Antimicrobial Chemotherapy, 58(3):622–626. [doi:10.1093/jac/dkl289]
Gul, S.T., Khan, A., 2007. Epidemiology and epizootology of brucellosis: a review. Pakistan Veterinary Journal, 27(3): 145–151.
Hassawi, D., Kharma, A., 2006. Antimicrobial activity of some medicinal plants against Candida albicans. Journal of Biological Science, 6(1):109–114. [doi:10.3923/jbs.2006.109.114]
Hsieh, P.C., Mau, J.L., Huang, S.H., 2001. Antimicrobial effect of various combinations of plant extracts. Food Microbiology, 18(1):35–43. [doi:10.1006/fmic.2000.0376]
Lothfipour, F., Nazemiyeh, H., Fathi-Azad, F., Garaei, N., Arami, S., 2008. Evaluation of antibacterial activities of some medicinal plants from north-west Iran. Iranian Journal of Basic Medical Sciences, 11(2):80–85.
Mahboobi, M., Shahcheraghi, F., Feizabadi, M.M., 2006. Bactericidal effects of essential oiIs from clove, lavender and geranium on multi-drug resistant isolates of Pseudomonas aeruginosa. Iranian Journal of Biotechnology, 4(2):137–140.
Mirjana, S., Nada, B., Valerija, D., 2004. Variability of Satureja cuneifolia Ten. essential oils and their antimicrobial activity depending on the stage of development. European Food Research and Technology, 218(4): 367–371. [doi:10.1007/s00217-003-0871-4]
Moazedi, A.A., Mirzaie Damabi, N., Seyyednejad, S.M., Zadkarami, M.R., Amirzargar, A., 2007. Spasmolytic effect of Petroselinum crispum (parsley) on rat’s ileum at different calcium chloride concentrations. Pakistan Journal of Biological Sciences, 10(22):4036–4042. [doi:10.3923/pjbs.2007.4036.4042]
NCCLS, 1993. Methods for Dilution in Antimicrobial Susceptibility Test. Approved Standard: M2–M5. National Committee for Clinical Laboratory Standards (NCCLS), Villanova, PA.
Nascimento, G.F.G., Locatelli, J., Freitas, C.P., Silva, L.G., 2000. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian Journal of Microbiology, 31(4):247–256. [doi:10.1590/S1517-83822000000400003]
Reuben, K.D., Abdulrahman, F.I., Akan, J.C., Usman, H., Sodipo, O.A., Egwu, G.O., 2008. Phytochemical screening and in vitro antimicrobial investigation of the methanolic extract of Croton zambesicus Muell ARG stem bark. European Journal of Scientific Research, 23(1):134–140.
Seyyednejad, M., Ebrahimzadeh, H., Talaie, A., 2001. Carbohydrate content in olive Zard c.v. and alternate bearing pattern. International Sugar Journal, 103(1226):84–87.
Turkmani, A., Ioannidis, A., Christidou, A., Psaroulaki, A., Loukaides, F., Tselentis, Y., 2006. In vitro susceptibilities of Brucella melitensis isolates to eleven antibiotics. Annals of Clinical Microbiology and Antimicrobials, 5:24 [doi:10.1186/1476-0711-5-24]
Valenza, G., Kallmann, B., Berend, A., Mlynski, R., Nockler, K., Kurzai, O., Frosch, M., Abele-Horn, M., 2006. Isolation of Brucella melitensis from a patient with hearing loss. European Journal of Clinical Microbiology & Infectious Diseases, 25(1):67–68. [doi:10.1007/s10096-006-0084-4]
Zakaria, Z., Sreenivasan, S., Mohamad, M., 2007. Antimicrobial activity of Piper ribesoides root extract against Staphylococcus aureus. Journal of Applied Biological Sciences, 1(3):87–90.
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Project supported by the finance grant provided by the vice chancellor of research of Shahid Chamran University, Ahvaz, Iran
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Motamedi, H., Darabpour, E., Gholipour, M. et al. In vitro assay for the anti-brucella activity of medicinal plants against tetracycline-resistant Brucella melitensis . J. Zhejiang Univ. Sci. B 11, 506–511 (2010). https://doi.org/10.1631/jzus.B0900365
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DOI: https://doi.org/10.1631/jzus.B0900365