Abstract
Factorial design and response surface techniques were used to design and optimize increasing P450 BM-3 expression in E. coli. Operational conditions for maximum production were determined with twelve parameters under consideration: the concentration of FeCl3, induction at OD578 (optical density measured at 578 nm), induction time and inoculum concentration. Initially, Plackett-Burman (PB) design was used to evaluate the process variables relevant in relation to P450 BM-3 production. Four statistically significant parameters for response were selected and utilized in order to optimize the process. With the 416C model of hybrid design, response surfaces were generated, and P450 BM-3 production was improved to 57.90×10−3 U/ml by the best combinations of the physicochemical parameters at optimum levels of 0.12 mg/L FeCl3, inoculum concentration of 2.10%, induction at OD578 equal to 1.07, and with 6.05 h of induction.
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Project (No. 30570411) supported by the National Natural Science Foundation of China
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Lu, Y., Mei, Lh. Optimization of fermentation conditions for P450 BM-3 monooxygenase production by hybrid design methodology. J. Zhejiang Univ. - Sci. B 8, 27–32 (2007). https://doi.org/10.1631/jzus.2007.B0027
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DOI: https://doi.org/10.1631/jzus.2007.B0027