Abstract
A FAST (fluorescence of advanced Maillard products and Soluble Tryptophan) method for identification of reconstituted milk made from skim milk powder in the fresh milk was developed. Considering milk and skim milk powders variations from different seasons and countries, milk was collected from different dairy farms in different seasons and skim milk powders were collected from different countries to measure the Tryptophan (Trp), advanced Maillard products (AMP) fluorescence values. The results showed that there were differences (P<0.01) between raw and reconstituted milk. The plot of values in each mixed level of raw and reconstituted milk had a correlation coefficient >0.97. The FAST method is a simple, rapid, low-cost and sensitive method enabling the detection of 5% reconstituted milk in fresh milk. The measurement of the Trp, AMP fluorescence values and calculation of the FAST index is a suitable method for large-scale monitoring of fresh milk samples.
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References
Birlouez-Aragon, I., Nicolas, M., Métais, A., Marchon, N., Grenier, J., Calvo, D., 1998. A rapid fluorimetric method to estimate the heat treatment of liquid milk.Int. Dairy J.,8:771–777.
Birlouez-Aragon, I., Sabat, P., Gouti, N., 2002. A new method for discriminating milk heat treatment.Int. Dairy J.,12:59–67.
Botelho, M.M., Valente-Mesquita, V.L., Oliveira, K.M.G., Polikarpov, I., Ferreira, S.T., 2000. Pressure denaturation of β-lactoglobulin: different stabilities of isoforms A and B, and an investigation of the Tanford transition.Eur. J. Biochem.,267:2235–2241.
Chen, F.T., Zang, J.H., 1992. Determination of milk proteins by capillary electrophoresis.J. AOAC Int.,75:905–909.
Corredig, M., Dalgleish, G., 1999. The mechanisms of the heat-induced interaction of whey proteins with casein micelles in milk.Int. Dairy J.,9:233–236.
Dalgleish, G., 1990. Denaturation and aggregation of serum proteins and caseins in heated milk.J. Agric. Food Chem.,38:1995–1999.
Erbersdobler, H.F., 1994. Effects of UHT heating of milk in an experimental plant on several indicators of heat treatment.J. Dairy Res.,61:305–309.
Henle, T., Walker, H., Krause, I., Klostermeyer, H., 1991. Efficient determination of individual Maillard compounds in heat-treated milk products by amino acid analysis.Int. Dairy J.,1:125–135.
Jing, H., Kitts, D.D., 2002. Chemical and biochemical properties of casein-sugar Maillard reaction products.Food Chem. Toxicol.,40:1007–1015.
Kolosta, M., Gasperik, J., Dronovsky, M., 2004. Thermal denaturation of whey proteins of ewe and goat milk.Bull Potravinarsk Vyskumu,43(1/2):1–13.
Labuza, T.P., Saltmarch, M., 1981. Kinetics of browning and protein quality loss in whey powders during the steady state and nonsteady state storage conditions.J. Dairy Sci.,47:92–113.
Leclère, J., Birlouez-Aragon, I., 2001. The fluorescence of advanced Maillard products is a good indicator of lysine damage during the Maillard Reaction.J. Agric. Food Chem.,49:4682–4687.
Lin, G.P., Rau, Y.H., Chen, Y.F., Chou, C.C., Fu, W.G., 2003. Measurements of dD and d18O stable isotope ratios in milk.J. Food Sci.,68(7):2192–2195.
Madkour, M.A.M., Moussa, B.M.I., 1989. The determination of milk powder added to whole milk.Nahrung,33(1):77–82.
Pagliarini, E., Lametti, S., Peri, C., Bonomi, F., 1990. An analytical approach to the evaluation of heat damage in commercial milks.J. Dairy Sci.,73:41–44.
Pompci, C., Rossi, M., Mare, F., 1988. Protein quality in commercial milk-based infant formulas.J. Food qual.,10(6):375–391.
Rehman, Z.U., Saeed, A., Zafar, S.I., 2000. Hydroxymethyl-furfural as an indicator for the detection of dried powder in liquid milk.Milchwissenschaft,55(5):256–257.
Resmini, P., Pellegrino, L., Hogenboom, J.A., Andreini, R., 1989. Thermal denaturation of whey protein in pasteurized milk. Fast evaluation by HPLC.Ital. J. Food Sci.,13:51–62.
Resmini, P., Pellegrino, L., Masotti, F., Tirelli, A., Prati, F., 1992. Detection of reconstituted milk powder in raw and in pasteurized milk by HPLC of furosine.Scienzae Tecnica Lattiero Casearia,43(3):169–186.
Rynne, N.M., Beresford, T.P., Kelly, A.L., Guinee, T.P., 2004. Effect of milk pasteurization temperature and in situ whey protein denaturation on the composition, texture and heat-induced functionality of half-fat Cheddar cheese.Int. Dairy J.,14(11):989–1001.
Valente-Mesquita, V.L., Botelho, M.M., Ferreira, S.T., 1998. Pressure-induced subunit dissociation and unfolding of β-lactoglobulin.Biophys. J.,75:471–476.
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Project (No. 2002BA518A06) supported by the Hi-Tech Research and Development Program (863) of China
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Guan, Rf., Liu, Dh., Ye, Xq. et al. Use of fluorometry for determination of skim milk powder adulteration in fresh milk. J Zheijang Univ Sci B 6, 1101–1106 (2005). https://doi.org/10.1631/jzus.2005.B1101
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DOI: https://doi.org/10.1631/jzus.2005.B1101