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Viability and in Vitro Germination of Johnsongrass (Sorghum Halepense) Pollen

Published online by Cambridge University Press:  20 January 2017

Ian C. Burke ,
John W. Wilcut  and
Nina S. Allen
Ian C. Burke*
Affiliation:
Crop Science Department, North Carolina State University, Raleigh, NC 27695-7620
John W. Wilcut
Affiliation:
Crop Science Department, North Carolina State University, Raleigh, NC 27695-7620
Nina S. Allen
Affiliation:
Botany Department, Box 7620, North Carolina State University, Raleigh, NC 27695-7620
*
Corresponding author's E-mail: icburke@wsu.edu
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Abstract

A high proportion of viable pollen grains must germinate to study the physiology of pollen growth to reduce the confounding effects of environmental influences on pollen germination. The objectives of this study were to evaluate the nuclear state and develop a suitable medium and culture method for in vitro germination of johnsongrass pollen. Johnsongrass pollen was trinucleate, and in vitro tests for pollen viability using Alexander's stain and a fluorochromatic reaction method (FCR) indicated johnsongrass pollen was viable (92.6 to 98.4%). A factorial treatment arrangement of four concentrations of sucrose, two concentrations of boric acid, and two concentrations of calcium nitrate were used to determine the optimum pollen-germination medium composition in suspension culture, agar culture, and cellophane membrane culture. Germination was highest in a suspension culture with a medium containing 0.3 M sucrose, 2.4 mM boric acid, and 3 mM calcium nitrate. Pollen germination using this medium was 78.9% when anthers were harvested just before anthesis.

Keywords

Johnsongrass, Sorghum halepense (L.) Pers. SORHAPollen testsagar culturesuspension culturecellophane membrane culture
Type
Research
Information
Weed Technology , Volume 21 , Issue 1 , March 2007 , pp. 23 - 29
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

Alexander, M. P. 1969. Differential staining of aborted and non-aborted pollen. Stain Technol. 44:117–122.Google Scholar
Alexander, M. P. and Ganeshan, S. 1990. An improved cellophane method for in vitro germination of recalcitrant pollen. Stain Technol. 64:225–227.Google Scholar
Ayyangar, G. N. R. and Rao, V. P. 1931. Studies in sorghum: I, anthesis and pollination. Indian J. Agric. Sci. 1:445–454.Google Scholar
Bar-Shalom, D. and Mattsson, O. 1977. Mode of hydration, an important factor in the germination of trinucleate pollen grains. Sven. Bot. Tidskr. 71:245–251.Google Scholar
Brewbaker, J. L. 1957. Pollen cytology and self-incompatibility systems in plants. J. Hered. 48:271–277.CrossRefGoogle Scholar
Brewbaker, J. L. 1959. Biology of the angiosperm pollen grain. Indian J. Genet. Plant Breed. 19:121–133.Google Scholar
Brewbaker, J. L. 1967. The distribution and phylogenetic significance of binucleate and trinucleate pollen grains in the angiosperms. Am. J. Bot. 54:1069–1083.Google Scholar
Brewbaker, J. L. and Kwack, B. H. 1963. The essential role of calcium ion in pollen germination and pollen tube growth. Am. J. Bot. 50:859–865.Google Scholar
Burton, J. D. 1997. Acetyl-coenzyme A carboxylase inhibitors. in Roe, R.M., Burton, J.D., Kuhr, R.J., eds. Herbicide Activity: Toxicology, Biochemistry and Molecular Biology. Burke, VA IOS Press. 187–205.Google Scholar
Coleman, A. W. and Goff, L. J. 1985. Applications of fluorochromes to pollen biology, I: mithramycin and 4′,6-diamidino-2-phenylindole (DAPI) as vital stains for quantitation of nuclear DNA. Stain Technol. 60:145–154.Google Scholar
Cheng, C. H. and McComb, J. A. 1992. In vitro germination of wheat pollen on raffinose medium. New Phytol. 120:459–462.Google Scholar
Crowe, J. H., Hoekstra, F. A., and Crowe, L. M. 1989. Membrane phase transitions are responsible for imbibitional damage in dry pollen. Proc. Natl. Acad. Sci. U S A. 86:520–523.Google Scholar
Doggett, H. 1988. Sorghum. New York Wiley. 512.Google Scholar
Fei, S. and Nelson, E. 2003. Estimation of pollen viability, shedding pattern, and longevity of creeping bentgrass on artificial media. Crop Sci. 43:2177–2181.Google Scholar
Gleason, H. A. and Cronquist, A. 1991. Manual of Vascular Plants. Bronx New York Botanical Garden. 815.Google Scholar
Graham, R. J. D. 1916. Pollination and cross-fertilization in the juar plant (Andropogon sorghum Brot.). India Dep. Agric. Mem. Bot. Ser. 8:201–216.Google Scholar
Heap, I. 2005. The International Survey of Herbicide Resistant Weeds. http://www.weedscience.com. Accessed: March 17, 2005.Google Scholar
Heslop-Harrison, J. and Heslop-Harrison, Y. 1970. Evaluation of pollen viability by enzymatically induced fluorescence: intra-cellular hydrolysis of fluorescein diacetate. Stain Technol. 45:115–122.CrossRefGoogle Scholar
Hoekstra, F. A. and Bruinsma, J. 1975. Respiration and vitality of binucleate and trinucleate pollen. Physiol. Plant. 34:221–225.Google Scholar
Holm, L. G., Plunknett, D. L., Pancho, J. V., and Herberger, J. P. 1991. The World's Worst Weeds, Distribution and Biology. Malabar, FL Kreger Publishing Company. 54–61.Google Scholar
Kearns, C. A. and Inouye, D. W. 1993. Techniques for pollination biologists. Niwot, CO University Press of Colorado. 583.Google Scholar
Lansac, A. R., Sullivan, C. Y., Johnson, B. E., and Lee, K. W. 1994. Viability and germination of the pollen of sorghum [Sorghum bicolor (L.) Moench]. Ann. Bot. 74:27–33.CrossRefGoogle Scholar
Leduc, N., Monnier, M., and Douglas, G. C. 1990. Germination of trinucleated pollen: formulation of a new medium for Capsella bursa-pastoris . Sex Plant Reprod. 3:228–235.Google Scholar
Letouze, A. and Gasquez, J. 2000. A pollen test to detect ACCase target-site resistance within Alopecurus myosuroides populations. Weed Res. 40:151–162.Google Scholar
McIntosh, M. S. 1983. Analysis of combined experiments. Agron. J. 75:153–155.Google Scholar
Muccifora, S., Bellani, L. M., and Gori, P. 2003. Ultrastructure, viability, and in vitro germination of the tricellular Sambucus nigra L. pollen. Int. J. Plant Sci. 164:855–860.CrossRefGoogle Scholar
Patel, M. L. and Patel, G. B. 1928. Studies in the Jowars of Gujarat, I: the Jowars of the Surat District. India Dep. Agric. Mem. Bot. Ser. 16:1–57.Google Scholar
Patil, R. C. and Goud, J. V. 1980. Viability of pollen and receptivity of stigma in sorghum. Indian J. Agric. Sci. 50:522–526.Google Scholar
Pline, W. A., Edmisten, K. L., Oliver, T., Wilcut, J. W., Wells, R., and Allen, N. S. 2002. Use of digital image analysis, viability stains, and germination assays to estimate conventional and glyphosate-resistant cotton pollen viability. Crop Sci. 42:2193–2200.Google Scholar
Richter, J. and Powles, B. P. 1993. Pollen expression of herbicide target site resistance genes in annual ryegrass (Lolium rigidum). Plant Physiol. 102:1037–1041.Google Scholar
Rosenow, D. T. and Clark, L. E. 1969. Description of off-types. in. Proceedings of the 6th Biennial Grain Sorghum Research and Utilization Conference. Amarillo, TX National Grain Sorghun Producers. 43–48.Google Scholar
Sanchez, R. L. and Smeltzer, D. G. 1965. Sorghum pollen viability. Crop Sci. 5:111–113.Google Scholar
SAS 1998. SAS/STAT User's Guide. Release 7.00. Cary, NC SAS Institute. 1028.Google Scholar
Shivanna, K. R. and Rangaswamy, N. S. 1992. Pollen Biology: A Laboratory Manual. New York Springer-Verlag. 123.Google Scholar
Stephens, J. C. and Quinby, J. R. 1934. Anthesis, pollination, and fertilization in sorghum. J. Agric. Res. 49:123–136.Google Scholar
Tuinstra, M. R. and Wedel, J. 2000. Estimation of pollen viability in grain sorghum. Crop Sci. 40:968–970.Google Scholar