Sertoli cell tumour in a neonate calf: an unusual congenital tumour

 

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Summary

Congenital testicular tumours are seldom reported in bovine species. This case report describes the clinical, sonographical, haematological, pathomorphological and immunohistological features of a Sertoli cell tumour in a neonatal German Holstein calf. Microscopically, the enlarged testicle was composed of neoplastic cells, which were packed in well-formed tubules. The mostly polygonal shaped cells had round to elongated nuclei and a scanty eosinophilic cytoplasm. Some cells were arranged perpendicularly to the light PAS-positive basement membrane. These cells were packed in broad sheets separated by dense fibrous stroma. Mitotic figures were present. The features described above are indicative of a Sertoli cell tumour. The contralateral testicle showed a well formed rete testis, fusiform cells and a dense central capillary convolute and haemorrhagic foci. The features are indicative of an extensive fibrosis and older haemorrhage. The neoplasia was immunopositive for vimentin, α-oestrogen receptor, α-inhibin and S-100 protein, but immunonegative for cytokeratine, CD30, progesterone receptor, α-fetoprotein, SALL4, OCT4 and glypican-3. The myco - toxicological investigations revealed the presence of residues of zea - ra lenone, deoxynivalenol, ochratoxin, HT2 toxin and their metabolites in feeds and urine of heavily pregnant cows of the herd. Furthermore, information is provided about oestrogen and testosterone levels of the affected and healthy neonatal calves. A possible influence of mycotoxins on the cancerogenesis is discussed.

Zusammenfassung

Berichte über kongenitale Hodentumore bei Rindern sind selten. Der Fallbericht beschreibt die klinischen, sonographischen, hämatologischen, pathomorphologischen und immuhistologischen Merkmale eines Sertoli-Zell-Tumor bei einem neugeborenen Deutsche-Holstein-Kalb. Histologisch wies der vergrößerte Hoden bindegewebig eingeteilte intratubuläre polygonale Tumorzellen mit runden bis fusiformen Kernen und leicht eosinophilem Zytoplasma auf. Einige Tumorzellen standen senkrecht zur Basalmembran. Die Tumorzellplatten waren durch ein fibröses Stroma septiert. Mitosen waren nachweisbar. Die genannten Merkmale sprechen für einen Sertoli-Zell-Tumor. Der kontralaterale Hoden zeigte ein gut ausgebildetes Rete testis, teils unreife fusiforme Zellen, zentral ein dichtes Gefäßkonvolut sowie fokale Einblutungen. Dies spricht für eine ausgedehnte Fibrose und ältere Einblutungen. Die Neoplasie war immunpositiv für Vimentin, α-Ö–strogen-Rezeptor, α-Inhibin und S-100 Protein, aber immunnegativ für Zytokeratin, CD30, Progesteronrezeptor, α-Fetoprotein, SALL4, OCT4 und Glypican-3. Mittels mykotoxikologischer Untersuchung konnten Zearalenon, Deoxynivalenol, Ochratoxin, HT2-Toxin bzw. deren Metaboliten in Futter- und Harnproben der hochtragenden Kühe der Herde nachgewiesen werden. Darüber hinaus wurden Informationen über den Ö–strogen- und Testosteronspiegel bei dem erkrankten Neonaten sowie bei neugeborenen gesunden Vergleichskälbern gewonnen. Eine mögliche Rolle der Mykotoxine in der Kanzerogenese wird diskutiert.

^* These authors contributed equally to this work.

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