Phytochemical study of Salvia grandiflora and Salvia officinalis leaves for establishing prospects for use in medical and pharmaceutical practice

Authors

DOI:

https://doi.org/10.15587/2519-4852.2020.197299

Keywords:

Salvia genus, non-pharmacopeia species, leaves, chemical composition, phenolic compounds

Abstract

The raw material base of medicinal plants in Ukraine is sufficient only for half of the pharmacopoeial species. Most part of the medicinal plants is growing in insufficient quantities and there is a need for their import. In conditions of import dependence and a shortage of domestic plant materials, the search for new sources of biologically active substances among the representatives of the flora of Ukraine is an urgent task of modern pharmaceutical science.

The aim of the work – conduct a comparative phytochemical study of the leaves of S. grandiflora and S. officinalis to establish the possibility of using the non-pharmacopeia species in pharmaceutical and medical practice.

Materials and methods. The objects of the study are the leaves of S. grandiflora and S. officinalis, which was harvested in the botanical garden of the Lviv National University named after I. Franko. The study of macro- and microelement composition in the leaves of S. officinalis and S. grandiflora was carried out by atomic emission spectrographic method. Determination of the qualitative composition and quantitative content of the main groups of biologically active substances was carried out by HPLC. Quantitative determination of phenolic compounds was also carried out by spectrophotometric method.

Results. The content of 15 micro and macro elements was found in both studied species. In the leaves of S. officinalis and S. grandiflora, 15 amino acids and 8 saponins were identified. Using HPLC, the qualitative composition and quantitative content of phenolic substances in the leaves of S. officinalis and S. grandiflora (13 and 9 compounds, respectively) was established.

Discussion. The dominant macro and micro elements in the studied species were silicon, phosphorus, magnesium, calcium, sodium, and potassium. The total content of trace elements in the leaves of S. grandiflora is 1.67 times greater than in the pharmacopeia plant S. officinalis. The dominant amino acids in the leaves of both species are glutamic acid, aspartic acid, valine and leucine.

The dominant saponins in the leaf of S. officinalis were ursolic and oleanolic acids, the total content of which is 75.82 %. In the leaves of S.grandiflora, ursolic and euskapic acids were dominant, with a total content of 63.25 %.

The total flavonoid content is higher in S. officinalis leaf and is 4.90 mg / g. The total content of hydroxycinnamic acids is highest in the leaf of S. grandiflora and is 4.49 mg / g, which is 221.18 % (2.21 times) higher than in the pharmacopeia plant S. officinalis (2.03 mg / g). The total highest content of caffeic acid derivatives prevails in the Salvia officinalis leaf (0.77 mg / g). The highest content of the sum of all detected phenolic compounds is specified for S. officinalis leaves and amounts to 6.93 mg / g.

Conclusions. As a result of a comparative phytochemical study of the leaves of S. grandiflora and S. officinalis, it was established that S. grandiflora is a promising species for introduction into medical and pharmaceutical practice, namely, as a source of phenolic compounds

Author Biographies

Mykhailo Myha, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

Postgraduate Student

Department of Pharmacognosy

Oleh Koshovyi, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

Doctor of Pharmaceutical Sciences, Professor, Head of Department

Department of Pharmacognosy

Olha Gamulya, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

PhD, Senior Lecturer

Department of Fundamental and Language Training

Tetiana Ilina, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

Doctor of Pharmaceutical Sciences, Professor

Department of Pharmacognosy

Natalia Borodina, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

PhD, Associate Professor

Department of Pharmacognosy

References

  1. Koshovyi, O. M. (2013). Suchasni pidkhody do stvorennia likarskykh zasobiv na osnovi roslyn rodiv Evkalipt ta Shavliia. Kharkiv: NFaU, 41.
  2. Minarchenko, V. M., Butko, A. Yu. (2017). Doslidzhennia vitchyznianoho rynku likarskykh zasobiv roslynnoho pokhodzhennia. Farmatsevtychnyi zhurnal, 1, 30–36. Available at: http://nbuv.gov.ua/UJRN/pharmazh_2017_1_5
  3. Nykytiuk, Yu. A. (2016). Orhanizatsiino-ekonomichni vazheli derzhavnoho stymuliuvannia importozamishchennia na rynku likarskoi roslynnoi syrovyny. Zbalansovane pryrodokorystuvannia, 4, 202–207.
  4. Tildesley, N., Kennedy, D., Perry, E., Ballard, C., Wesnes, K., Scholey, A. (2005). Positive modulation of mood and cognitive performance following administration of acute doses of Salvia lavandulaefolia essential oil to healthy young volunteers. Physiology & Behavior, 83 (5), 699–709. doi: http://doi.org/10.1016/j.physbeh.2004.09.010
  5. Koshevoi, O. N. (2011). Amino-acid and monosaccharide compositions of Salvia officinalis leaves. Chemistry of Natural Compounds, 47 (3), 492–493. doi: http://doi.org/10.1007/s10600-011-9976-3
  6. Cherniavskyi, A. V., Terenteva, N. H. (1987). Yzuchenye vydov roda Salvia L. Flori Ukrayni pry pomoshchy dyskrymynalnoho analyza. Ukrainskyi botanichnyi zhurnal, 5 (44), 63–67.
  7. Lopresti, A. L. (2016). Salvia (Sage): A Review of its Potential Cognitive-Enhancing and Protective Effects. Drugs in R&D, 17 (1), 53–64. doi: http://doi.org/10.1007/s40268-016-0157-5
  8. Myha, M. M., Koshovyi, O. M., Ilina, T. V., Borodina, N. V., Skybitska, M. I. (2019). Research in phenolic compounds in leaves of non-pharmacopoeial species of the genus Salvia from Ukrainian flora. Current Issues in Pharmacy and Medicine: Science and Practice, 3, 291–297. doi: http://doi.org/10.14739/2409-2932.2019.3.184191
  9. Baranauskiene, R., Dambrauskiene, E., Venskutonis, P. R., Viskelis, P. (2011). Influence of harvesting time on the yield and chemical composition of sage (Salvia officinalis L.). Foodbalt, 104–109.
  10. Raal, A., Orav, A., Arak, E. (2007). Composition of the essential oil ofSalvia officinalisL. from various European countries. Natural Product Research, 21 (5), 406–411. doi: http://doi.org/10.1080/14786410500528478
  11. Kovalenko, V. N. (Ed.) (2014). Kompendyum 2014 – lekarstvennie preparaty. Kyiv: MORYON, 2700.
  12. Xu, J., Wei, K., Zhang, G., Lei, L., Yang, D., Wang, W. et. al. (2018). Ethnopharmacology, phytochemistry, and pharmacology of Chinese Salvia species: A review. Journal of Ethnopharmacology, 225, 18–30. doi: http://doi.org/10.1016/j.jep.2018.06.029
  13. Firuzi, O., Miri, R., Asadollahi, M., Eslami, S., Jassbi, A. R. (2013). Cytotoxic, antioxidant and antimicrobial activities and phenolic contents of eleven Salvia species from Iran. Iranian Journal of Pharmaceutical Research, 12, 801–810.
  14. Mahdizadeh, R., Moeln, S., Soltani, N., Malekzadeh, K., Moein, M. (2018). Study of molecular mechanism of Salvia species in prevention of diabetes. International Journal of Pharmaceutical Science and Research, 9, 4512–4521.
  15. Wu, Y.-B., Ni, Z.-Y., Shi, Q.-W., Dong, M., Kiyota, H., Gu, Y.-C., Cong, B. (2012). Constituents from Salvia Species and Their Biological Activities. Chemical Reviews, 112 (11), 5967–6026. doi: http://doi.org/10.1021/cr200058f
  16. Dobrochaeva, D. N., Kotov, M. I., Prokudin, Y. N., Barbarich, A. I. (1999). Key to Higher Plants of Ukraine. Kyiv: Naukova Dumka, 546.
  17. Eidi, M., Eidi, A., Bahar, M. (2006). Effects of Salvia officinalis L. (sage) leaves on memory retention and its interaction with the cholinergic system in rats. Nutrition, 22 (3), 321–326. doi: http://doi.org/10.1016/j.nut.2005.06.010
  18. Koshovyi, O. M., Komisarenko, A. M., Kovalova, A. M., Mudryk, I. M. (2005). Mikroelementnyi, aminokyslotnyi ta polisakharydnyi sklad lystia evkalipta. Fitoterapiia. Chasopys, 3, 59–62.
  19. Osmachko, A. P., Kovaleva, A. M., Ili'ina, T. V., Koshovyi, O. N., Komisarcnko, A. M., Akhmedov, E. Yu. (2017). Study of macro- and microelements composition of Veronica longifolia L. herb and Veronica teucrium L. herb and rhizomes, and extracts obtained from these species. Azerbaijan Pharmaceutical and Pharmacotherapy Journal, 1, 24–28. Available at: http://dspace.nuph.edu.ua/handle/123456789/20611
  20. Derzhavna Farmakopeia Ukrainy. Vol. 1 (2015). Kharkiv: Derzhavne pidpryiemstvo «Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv», 1128.
  21. Eidi, M., Eidi, A., Zamanizadeh, H. (2005). Effect of Salvia officinalis L. leaves on serum glucose and insulin in healthy and streptozotocin-induced diabetic rats. Journal of Ethnopharmacology, 100 (3), 310–313. doi: http://doi.org/10.1016/j.jep.2005.03.008
  22. Jassbi, A. R., Zare, S., Firuzi, O., Xiao, J. (2015). Bioactive phytochemicals from shoots and roots of Salvia species. Phytochemistry Reviews, 15 (5), 829–867. doi: http://doi.org/10.1007/s11101-015-9427-z
  23. Koshovyi, O. M., Zagayko, A. L., Kolychev, I. O., Akhmedov, E. Yu., Komissarenko, A. N. (2016). Phytochemical study of the dry extract from bilberry leaves. Azerbaijan Pharmaceutical and Pharmacotherapy Journal, 1, 18–23.
  24. Jash, S. K., Gorai, D., Roy, R. (2016). Salvia genus and triterpenoids. International Journal Of Pharmaceutical Sciences And Research, 7, 4710–4732. doi: http://doi.org/10.13040/ijpsr.0975-8232.7(12).4710-32
  25. Shynkovenko, I. L., Ilyina, T. V., Kovalyova, A. M., Goryacha, O. V., Golembiovska, O. I., Koshovyi, O. M. (2018). Saponins of the extracts of Galium aparine and Galium verum. News of Pharmacy, 4 (96), 16–23. doi: http://doi.org/10.24959/nphj.18.2225
  26. Koshovyi, O. M. (2012). Fenolnyi sklad deiakykh predstavnykiv pidrodu Sclarea rodu Salvia. Aktualni pytannia farmatsevtychnoi i medychnoi nauky ta praktyky, 3, 11–14. Available at: http://nbuv.gov.ua/UJRN/apfimntp_2012_3_5
  27. Ilina, T., Kashpur, N., Granica, S., Bazylko, A., Shinkovenko, I., Kovalyova, A. et. al. (2019). Phytochemical Profiles and In Vitro Immunomodulatory Activity of Ethanolic Extracts from Galium aparine L. Plants, 8 (12), 541. doi: http://doi.org/10.3390/plants8120541
  28. Kamatou, G., Viljoen, A., Steenkamp, P. (2010). Antioxidant, anti-inflammatory activities and HPLC analysis of South African Salvia species. Planta Medica, 76 (12). doi: http://doi.org/10.1055/s-0030-1264458
  29. Koshevoi, O. N., Vovk, G. V., Akhmedov, E. Iu., Komisarenko, A. N. (2015). Issledovanie khimicheskogo sostava i farmakologicheskoi aktivnosti ekstraktov, poluchennykh pri kompleksnoi pererabotke listev shalfeia lekarstvennogo. Azerbaidzhanskii farmacevticheskii i farmakoterapevticheskii zhurnal, 1, 30–34.
  30. Koshovyi, O. M., Perederii, Ye. O., Kovalova, A. M., Komisarenko, A. M. (2010). Doslidzhennia fenolnykh spoluk lystia shavlii likarskoi. Farmatsevtychnyi chasopys, 1, 17–19.

Downloads

Published

2020-02-28

How to Cite

Myha, M., Koshovyi, O., Gamulya, O., Ilina, T., Borodina, N., & Vlasova, I. (2020). Phytochemical study of Salvia grandiflora and Salvia officinalis leaves for establishing prospects for use in medical and pharmaceutical practice. ScienceRise: Pharmaceutical Science, (1 (23), 23–28. https://doi.org/10.15587/2519-4852.2020.197299

Issue

No. 1 (23) (2020)

Section

Pharmaceutical Science

License

Copyright (c) 2020 Mykhailo Myha, Oleh Koshovyi, Olha Gamulya, Tetiana Ilina, Natalia Borodina, Inna Vlasova

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Our journal abides by the Creative Commons CC BY copyright rights and permissions for open access journals.