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In Vivo Dopamine Detection and Single Unit Recordings Using Intracortical Glassy Carbon Microelectrode Arrays

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Abstract

In this study, we present a 4-channel intracortical glassy carbon (GC) microelectrode array on a flexible substrate for the simultaneous in vivo neural activity recording and dopamine (DA) concentration measurement at four different brain locations (220μm vertical spacing). The ability of GC microelectrodes to detect DA was firstly assessed in vitro in phosphate-buffered saline solution and then validated in vivo measuring spontaneous DA concentration in the Striatum of European Starling songbird through fast scan cyclic voltammetry(FSCV). The capability of GC microelectrode arrays and commercial penetrating metal microelectrode arrays to record neural activity from the Caudomedial Neostriatum of European starling songbird was compared. Preliminary results demonstrated the ability of GC microelectrodes in detecting neurotransmitters release and recording neural activity in vivo. GC microelectrodes array may, therefore, offer a new opportunity to understand the intimate relations linking electrophysiological parameters with neurotransmitters release.

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References

  1. M. van Spronsen and C. C. Hoogenraad, Curr Neurol Neurosci Rep. 10(3), 207 (2010).

    Article  Google Scholar 

  2. R. Brisch, A. Saniotis, R. Wolf, H. Bielau, H. G. Bernstein, J. Steiner, B. Bogerts, K. Braun, Z. Jankowski, J. Kumaratilake, M. Henneberg, T. Gos, Front Psychiatry 5, 47 (2014).

    Google Scholar 

  3. J. Lotharius and P. Brundin, Nature Reviews Neuroscience 3(12), 932 (2002).

    Article  CAS  Google Scholar 

  4. N. D. Volkow, J.S.Fowler, G. J. Wang, Behav Pharmacol. 13(5–6), 355 (2002).

    Article  CAS  Google Scholar 

  5. E. Castagnola, S. Carli, M. Vomero, A. Scarpellini, M. Prato, N. Goshi, L. Fadiga, S. Kassegne, D. Ricci, Biointerphases 12(3), 031002 (2017).

    Article  Google Scholar 

  6. M. L. Mundroff and M. R. Wightman, Curr Protoc Neurosci Chapter 6: Unit 6.14, (2002).

  7. P. S. Cahill, Q. D. Walker, J. M. Finnegan, G. E. Mickelson, E. R. Travis, M. R. Wightman, Anal Chem 68, 3180 (1996).

    Article  CAS  Google Scholar 

  8. I. M. Taylor, E. M. Robbins, K. A. Catt, P. A. Cody, C. L. Happe, X.T. Cui, Biosens Bioelectron 89(Pt 1), 400 (2017).

    Article  CAS  Google Scholar 

  9. N. Xiao and B. J. Venton, Anal Chem. 84(18), 7816 (2012).

    Article  CAS  Google Scholar 

  10. W.Yi, Y. Yang, P. Hashemi, M. Ming-Cheng Cheng, Biomed Microdevices 18, 112 (2016).

    Article  Google Scholar 

  11. M. K. Zachek, P. Takmakov, B. Moody, R. M. Wightman, G. S. McCarty, Anal Chem 81(15), 6258 (2009).

    Article  CAS  Google Scholar 

  12. M. Vomero, E. Castagnola, F. Ciarpella, E. Maggiolini, N. Goshi, E. Zucchini, S. Carli, L. Fadiga, S. Kassegne, D. Ricci, Scientific Reports 7, 40332 (2016).

    Article  Google Scholar 

  13. M.Vomero, P.van Niekerk, V. Nguyen, N.Gong, M. Hirabayashi, A. Cinopri, K. Logan, A. Moghadasi, P.Varma and S. Kassegne, J Micromechanic Microengineer 26, 2 (2016).

    Article  Google Scholar 

  14. K. Simonyan, B. Horwitz, and E. D. Jarvis, Brain Lang 122(3), 142 (2012).

    Article  Google Scholar 

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Castagnola, E., Vahidi, N.W., Nimbalkar, S. et al. In Vivo Dopamine Detection and Single Unit Recordings Using Intracortical Glassy Carbon Microelectrode Arrays. MRS Advances 3, 1629–1634 (2018). https://doi.org/10.1557/adv.2018.98

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  • DOI: https://doi.org/10.1557/adv.2018.98

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