Development of Biodegradable Polymer Scaffolds using Co-Extrusion

Abstract

A methodology for the preparation of porous scaffolds for tissue engineering using co-extrusion is presented. Poly(ε-caprolactone) is blended with poly(ethylene oxide) in a twin-screw extruder to form a two-phase material with micrometer-sized domains. Selective dissolution of the poly(ethylene oxide) with water results in a porous material. This method of polymer extrusion permits the preparation of scaffolds having continuous void space and controlled characteristic length scales without the use of potentially toxic organic solvents. A range of blend volume fractions results in co-continuous networks of polymer and void spaces. Annealing studies demonstrate that the characteristic pore size may be increased to larger than 100 μm. The mechanical properties of the scaffolds are characterized by a compressive modulus on the order of 1 MPa at low strains and approximately 10 MPa at higher strains. The results of osteoblast seeding suggest it is possible to use co-extrusion to prepare polymer scaffolds without the introduction of toxic contaminants.