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Direct Observation of Calcium Oxalate Monohydrate Precipitation at Phospholipid Monolayers with Brewster Angle Microscopy

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Abstract

The precipitation of calcium oxalate monohydrate (COM) at phospholipid monolayers has been observed in-situ by Brewster angle microscopy (BAM). A monolayer of 1,2-dipalmitoylsn-glycero-3-phosphocholine (DPPC) compressed to a LC state over a calcium oxalate subphase shows the growth of COM as very bright spots. The identity of COM was confirmed in a transferred film by scanning electron microscopy. BAM can also be used to determine where COM precipitates when the monolayer has two phases at equilibrium. Monolayers of DPPC and 1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (DPPG) crystallize COM at phase boundaries. In addition, phase separated binary phospholipid mixtures of DPPC and 1,2-dimiristoyl-sn-glycero-3-phosphocholine (DMPC) have been prepared and monitored by BAM. The crystal growth in this case is confined to domains of DPPC due to its ability to form a liquid condensed phase.

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References

  1. E. L. Prien and E. L. Prien, Am. J. Med. 45, 654 (1968).

    Article  CAS  Google Scholar 

  2. A. Smesko, R. P. Singh, A. C. Lanzalaco, and G. H. Nancollas, Colloids and Surfaces 30, 361 (1988).

    Article  CAS  Google Scholar 

  3. S. R. Khan and R. L. Hackett, J. Urol. 150, 239 (1993).

    Article  CAS  Google Scholar 

  4. W. H. Boyce and F. K. Garvey, J. Urol. 76, 213 (1956)

    Article  CAS  Google Scholar 

  5. S. Whipps, S. R. Khan, F. J. O’Palko, R. Backov, and D. R. Talham, J. Cryst. Growth. 192, 243 (1998)

    Article  CAS  Google Scholar 

  6. R. Backov, S. R. Khan, C. Mingotaud, K. Byer, C. M. Lee, and D. R. Talham, J. Am. Soc. Neph. 10, S359 (1999).

    CAS  Google Scholar 

  7. R. Backov, C. M. Lee, S. R. Khan, C. Mingotaud, G. E. Fanucci, and D. R. Talham, Langmuir 16, 6013 (2000)

    Article  CAS  Google Scholar 

  8. S. R. Khan, P. A. Glenton, R. Backov, and D. R. Talham, Kidney International 62, 2062 (2002).

    Article  CAS  Google Scholar 

  9. E. Loste, E. Diaz-Marti, A. Zarbakhsh, and F. C. Meldrum, Langmuir 19, 2830 (2003)

    Article  CAS  Google Scholar 

  10. S. Koppenol, H. Yu, and G. Zografi, J. Colloid Interface Sci. 189, 158 (1997)

  11. B. M. Discher, W. R. Schief, V. Vogel, and S. B. Hall, Biophys. J. 77, 2051 (1999)

    Article  CAS  Google Scholar 

  12. C. K. Park, F. J. Schmitt, L. Evert, D. K. Schwartz, J. N. Israelachvili, and C. M. Knobler, Langmuir 15, 202 (1999)

    Article  CAS  Google Scholar 

  13. A. Gopal and K. Y. C. Lee J. Phys. Chem. 105 (2001).

  14. Y. F. Dufrene, W. R. Barger, J.-B. D. Green, and G. U. Lee, Langmuir 13, 4779 (1997)

    Article  CAS  Google Scholar 

  15. I. Reviakine, A. Simon, and A. Brisson, Langmuir 16, 1473 (2000)

    Article  CAS  Google Scholar 

  16. P. Moraille and A. Badia, Langmuir 18, 4414 (2002)

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, University of Florida, 32611-7200, Gainesville, Florida, USA

    Isa O. Benítez, Rénal Backov & Daniel R. Talham

  2. Department of Pathology, University of Florida, 32610, Gainesville, Florida, USA

    Saeed R. Khan

Authors
  1. Isa O. Benítez
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  2. Rénal Backov
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  3. Saeed R. Khan
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  4. Daniel R. Talham
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Benítez, I.O., Backov, R., Khan, S.R. et al. Direct Observation of Calcium Oxalate Monohydrate Precipitation at Phospholipid Monolayers with Brewster Angle Microscopy. MRS Online Proceedings Library 774, 59 (2003). https://doi.org/10.1557/PROC-774-O5.9

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  • DOI: https://doi.org/10.1557/PROC-774-O5.9

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