Abstract
Primary rat bone marrow cells were cultured on bacteriological grade polystyrene dishes which had been treated in selected areas with concentrated sulphuric acid. X-ray photoelectron spectroscopy, angle-resolved and imaging modes, and atomic force microscopy showed that the acid treatment brought about both chemical and topographical changes in the substratum surface. While the bone marrow cells attached to both treated and untreated areas of the surfaces of the dishes, the distribution of early mineralized extracellular matrix in these areas was reproducibily different. Thus, using extracellular matrix deposition as a marker, modification of the polymer surface resulted in the cells adhering to treated and untreated areas exhibiting different phenotypes.
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Acknowledgements
We are grateful to Bob Chernecky for assistance with the scanning electron microscopy. Financial support was kindly provided by both the Ontario-Rhone Alpes collaborative scheme and the Ontario Centre for Materials Research.
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Peel, S.A.F., Sodhi, R.N., Duc, T.M. et al. Polymer Surface Modification Causes Change in Phenotypic Expression of Primary Bone Cells. MRS Online Proceedings Library 252, 71–77 (1991). https://doi.org/10.1557/PROC-252-71
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DOI: https://doi.org/10.1557/PROC-252-71