Abstract
A genetically modified M13 bacteriophage template was used to biomineralize ZnO. A peptide, EAHVMHKVAPRP [1], with a known affinity for ZnO was genetically displayed on each of five copies of the pIII protein located at one tip of the M13 virus. Site-directed assembly using this pIII peptide fusion was studied using a variety of precursor concentrations, incubation times, and phage concentrations. For comparision, free ZnO-binding peptides were also used to biomineralize ZnO. Isolated, polydisperse, spherical ZnO nanoparticles were formed at all mineralization conditions containing the ZnO-binding M13 bacteriophage, whereas free peptide mineralization resulted in smaller, more irregularly shaped particles which agglomerated at longer incubation times. These studies are preliminary experiments in the investigation of ZnO biomineralization on the various structural proteins of the M13 bacteriophage and cooperative effects which occur between neighboring peptides.
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References
M. Umetsu, M. Mizuta, K. Tsumoto, S. Ohara, S. Takami, H. Watanabe, I. Kumagai, and T. Adschiri, “Bioassisted room-temperature immobilization and mineralization of zinc oxide - The structural ordering of ZnO nanoparticles into a flower-type morphology,” Advanced Materials, vol. 17, pp. 2571-+, Nov 4 2005. 10.1002/adma.200500863
P. Gao, C. Ying, S. Q. Wang, L. N. Ye, Q. X. Guo, and Y. Xie, “Low temperature hydrothermal synthesis of ZnO nanodisk arrays utilizing self-assembly of surfactant molecules at solid-liquid interfaces,” Journal of Nanoparticle Research, vol. 8, pp. 131–136, Feb 2006. 10.1007/s11051-005-5130-0
A. Umar, M. S. Chauhan, S. Chauhan, R. Kumar, G. Kumar, S. A. Al-Sayari, S. W. Hwang, and A. Al-Hajry, “Large-scale synthesis of ZnO balls made of fluffy thin nanosheets by simple solution process: Structural, optical and photocatalytic properties,” Journal of Colloid and Interface Science, vol. 363, pp. 521–528, Nov 15 2011. 10.1016/j.jcis.2011.07.058
M. M. Tomczak, M. K. Gupta, L. F. Drummy, S. M. Rozenzhak, and R. R. Nalk, “Morphological control and assembly of zinc oxide using a biotemplate,” Acta Biomaterialia, vol. 5, pp. 876–882, Mar 2009. 10.1016/j.actbio.2008.11.011
Z. Huang, D. Yan, M. Yang, X. Liao, Y. Kang, G. Yin, Y. Yao, and B. Hao, “Preparation and characterization of the biomineralized zinc oxide particles in spider silk peptides,” Journal of Colloid and Interface Science, vol. 325, pp. 356–362, Sep 15 2008. 10.1016/j.jcis.2008.05.040
Z. Wei, Y. Maeda, and H. Matsui, “Discovery of Catalytic Peptides for Inorganic Nanocrystal Synthesis by a Combinatorial Phage Display Approach,” Angewandte Chemie-International Edition, vol. 50, pp. 10585–10588, 2011 2011. 10.1002/anie.201102582
C. E. Flynn, C. B. Mao, A. Hayhurst, J. L. Williams, G. Georgiou, B. Iverson, and A. M. Belcher, “Synthesis and organization of nanoscale II-VI semiconductor materials using evolved peptide specificity and viral capsid assembly,” Journal of Materials Chemistry, vol. 13, pp. 2414–2421, 2003. 10.1039/B307593A
I. A. Banerjee, G. Muniz, S. Y. Lee, and H. Matsui, “Mineralization of semiconductor nanocrystals on peptide-coated bionanotubes and their pH-dependent morphology changes,” Journal of Nanoscience and Nanotechnology, vol. 7, pp. 2287–2292, Jul 2007. 10.1166/jnn.2007.642
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The authors would like to thank the Central Facility for Advanced Microscopy and Microanalysis (CFAMM) at UCR for training and assistance with transmission electron microscopy usage.
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Moon, C.H., Haberer, E.D. Facile Assembly of ZnO Nanoparticles Based on M13 Bacteriophage. MRS Online Proceedings Library 1461, 1–6 (2012). https://doi.org/10.1557/opl.2012.1372
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DOI: https://doi.org/10.1557/opl.2012.1372