Null mutation of exocyst complex component 3-like does not affect vascular development in mice

Satsuki Takashima; Eiichi Okamura; Yusuke Ichiyama; Kiyoto Nishi; Akio Shimizu; Chisato Watanabe; Masanaga Muto; Shoma Matsumoto; Setsuko Tsukiyama-Fujii; Tomoyuki Tsukiyama; Hisakazu Ogita; Eiichiro Nishi; Masahito Ohji; Fumihiro Sugiyama; Satoru Takahashi; Seiya Mizuno; Ken-ichi Mizutani; Masatsugu Ema

doi:10.1538/expanim.23-0105

Original

Null mutation of exocyst complex component 3-like does not affect vascular development in mice

Satsuki Takashima, Eiichi Okamura, Yusuke Ichiyama, Kiyoto Nishi, Akio Shimizu, Chisato Watanabe, Masanaga Muto, Shoma Matsumoto, Setsuko Tsukiyama-Fujii, Tomoyuki Tsukiyama, Hisakazu Ogita, Eiichiro Nishi, Masahito Ohji, Fumihiro Sugiyama, Satoru Takahashi, Seiya Mizuno, Ken-ichi Mizutani, Masatsugu Ema

Author information

Satsuki Takashima
Department of Stem Cells and Human Disease Models, Research Center for Animal Life Science, Shiga University of Medical Science, Seta, Tsukinowa-cho, Otsu, Shiga 520-2192, Japan
Eiichi Okamura
Department of Stem Cells and Human Disease Models, Research Center for Animal Life Science, Shiga University of Medical Science, Seta, Tsukinowa-cho, Otsu, Shiga 520-2192, Japan
Yusuke Ichiyama
Department of Ophthalmology, Shiga University of Medical Science, Seta, Tsukinowa-cho, Otsu, Shiga 520-2192, Japan
Kiyoto Nishi
Department of Pharmacology, Shiga University of Medical Science, Seta, Tsukinowa-cho, Otsu, Shiga 520-2192, Japan
Akio Shimizu
Division of Molecular Medical Biochemistry, Department of Biochemistry and Molecular Biology, Shiga University of Medical Science, Seta, Tsukinowa-cho, Otsu, Shiga 520-2192, Japan
Chisato Watanabe
Department of Stem Cells and Human Disease Models, Research Center for Animal Life Science, Shiga University of Medical Science, Seta, Tsukinowa-cho, Otsu, Shiga 520-2192, Japan
Masanaga Muto
Department of Stem Cells and Human Disease Models, Research Center for Animal Life Science, Shiga University of Medical Science, Seta, Tsukinowa-cho, Otsu, Shiga 520-2192, Japan
Shoma Matsumoto
Department of Stem Cells and Human Disease Models, Research Center for Animal Life Science, Shiga University of Medical Science, Seta, Tsukinowa-cho, Otsu, Shiga 520-2192, Japan
Setsuko Tsukiyama-Fujii
Department of Stem Cells and Human Disease Models, Research Center for Animal Life Science, Shiga University of Medical Science, Seta, Tsukinowa-cho, Otsu, Shiga 520-2192, Japan
Tomoyuki Tsukiyama
Department of Stem Cells and Human Disease Models, Research Center for Animal Life Science, Shiga University of Medical Science, Seta, Tsukinowa-cho, Otsu, Shiga 520-2192, Japan
Hisakazu Ogita
Division of Molecular Medical Biochemistry, Department of Biochemistry and Molecular Biology, Shiga University of Medical Science, Seta, Tsukinowa-cho, Otsu, Shiga 520-2192, Japan
Eiichiro Nishi
Department of Pharmacology, Shiga University of Medical Science, Seta, Tsukinowa-cho, Otsu, Shiga 520-2192, Japan
Masahito Ohji
Department of Ophthalmology, Shiga University of Medical Science, Seta, Tsukinowa-cho, Otsu, Shiga 520-2192, Japan
Fumihiro Sugiyama
Laboratory Animal Resource Center in Transborder Medical Research Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan
Satoru Takahashi
Laboratory Animal Resource Center in Transborder Medical Research Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan
Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan
International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan
Seiya Mizuno
Laboratory Animal Resource Center in Transborder Medical Research Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan
Ken-ichi Mizutani
Laboratory of Stem Cell Biology, Graduate School of Pharmaceutical Sciences, Kobe Gakuin University, 1-1-3 Minatojima, Chuo-ku, Kobe, Hyogo 650-8586, Japan
Masatsugu Ema
Department of Stem Cells and Human Disease Models, Research Center for Animal Life Science, Shiga University of Medical Science, Seta, Tsukinowa-cho, Otsu, Shiga 520-2192, Japan

Keywords: cardiovascular development, cholesterol, exocyst complex, exocyst complex component 3-like, insulin secretion

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Supplementary material

2024 Volume 73 Issue 1 Pages 93-100

DOI https://doi.org/10.1538/expanim.23-0105

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Abstract

Exocyst is an octameric protein complex implicated in exocytosis. The exocyst complex is highly conserved among mammalian species, but the physiological function of each subunit in exocyst remains unclear. Previously, we identified exocyst complex component 3-like (Exoc3l) as a gene abundantly expressed in embryonic endothelial cells and implicated in the process of angiogenesis in human umbilical cord endothelial cells. Here, to reveal the physiological roles of Exoc3l during development, we generated Exoc3l knockout (KO) mice by genome editing with CRISPR/Cas9. Exoc3l KO mice were viable and showed no significant phenotype in embryonic angiogenesis or postnatal retinal angiogenesis. Exoc3l KO mice also showed no significant alteration in cholesterol homeostasis or insulin secretion, although several reports suggest an association of Exoc3l with these processes. Despite the implied roles, Exoc3l KO mice exhibited no apparent phenotype in vascular development, cholesterol homeostasis, or insulin secretion.

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