Abstract
Due to their numerous advantages, baculovirus expression vector systems (BEVS) have been widely used to express recombinant proteins for different purposes. Different strategies have been adopted to increase recombinant protein production. In this study, we transiently or stably expressed mouse c-Myc in High Five cells using a commercial pIB/V5 vector. Under the control of the OpIE2 promoter, this vector could enhance recombinant protein production. We found that transient expression of c-Myc in High Five cells improved recombinant protein production. Furthermore, we established two stable cell lines, High Five-c-Myc #1 and High Five-c-Myc #2, that stably expressed mouse c-Myc. We further found that the expression level of the recombinant protein was increased in these stable cell lines compared to control cell lines. These data indicate that overexpressing c-Myc in cells is a promising way to improve recombinant protein production in BEVS.
Acknowledgments
This work was supported by the National Natural Science Foundation of China (81502621 and 81502088), the Natural Science Foundation of Jiangsu Province (BK20140539), and the Start-Up Research Funding of Jiangsu University for Distinguished Scholars (15JDG021 and 14JDG065).
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