Abstract
In this study, electrochemical behavior of phytohormone abscisic acid (ABA) at the bismuth-film electrode (BiFE) has been investigated using square-wave cathodic stripping voltammetry. BiFE was prepared ex situ on a supporting pencil-lead graphite electrode. ABA yielded a well-defined voltammetric response in phosphate buffer, pH 3.0 at -1.08 V (vs. Ag/AgCl) (a pre-concentration step being carried out at a fixed potential of -0.80 V for 120 s). The process could be used to determine ABA concentrations in the range 0.756–15.08 μm, with a detection limit of 0.209 μm (55.24 ng/ml). As an example, the practical applicability of pencil-lead BiFE was tested with the measurement of ABA in seed samples of maize plant (Zea mays L.).
©2011 by Walter de Gruyter Berlin New York
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