Abstract
Diet analysis is an essential part in understanding the biology of a species and functioning of ecosystems. Traditional morphological identification of undigested remains in the scats and molecular analyses of prey species’ DNA have previously been used to assess diet. In the present study, caracal diet in the Abbasabad Wildlife Refuge, Central Iran, was investigated using both molecular and morphological methods. We collected 22 scat samples from caracal dens in the region. Feces were washed on sieves and their remaining components were morphologically identified. We also targeted a 307-base pair fragment of the cytochrome b gene to amplify and sequence the species’ DNA. Morphological analyses revealed that 76% of the diet comprised rodent species. We identified a total of nine prey taxa using the molecular method, including six rodents, one hare, one hedgehog and one wild goat. There was a general agreement between the molecular and morphological results; however, molecular methods tended to allow a better identification of the prey species. Therefore, the DNA-based approach acts as a valuable complement to current morphological methods in the study of a rare felid’s diet when no hair reference library exists.
References
Akbari, H., A. Sadeghi, M.S. Farhadinia, M.S. Golmohammadi and V. Hojati. 2010. Identification of Mammalian fauna of abbasabad wildlife refuge Naeen, Isfahan Province, Iran. J. Anim. Biol. 2: 19–31.Search in Google Scholar
Avenant, N.L. and J.A.J. Nel. 1997. Prey use by syntopic carnivores in a strandveld ecosystem. S. Afr. J. Wildl. Res. 27: 86–93.Search in Google Scholar
Avenant, N.L. and J.A.J. Nel. 2002. Among habitat variation in prey availability and use by caracal Felis caracal. Mamm. Biol. 67: 18–33.10.1078/1616-5047-00002Search in Google Scholar
Avgan, B., P. Henschel and A. Ghoddousi. 2016. Caracal caracal (errata version published in 2016). The IUCN Red List of Threatened Species 2016: e.T3847A102424310. http://dx.doi.org/10.2305/IUCN.UK.2016-2.RLTS.T3847A50650230.en. Downloaded on 12 November 2017.10.2305/IUCN.UK.2016-2.RLTS.T3847A50650230.enSearch in Google Scholar
Balestrieri, A., L. Remonti and C. Prigioni. 2011. Assessing carnivore diet by faecal samples and stomach contents: a case study with Alpine red foxes. Cent. Eur. J. Biol. 6: 283–292.10.2478/s11535-010-0106-1Search in Google Scholar
Bothma, J.d.P. 1965. Random observations of the food habits of certain carnivora in South Africa. Fauna Flora 16: 16–22.Search in Google Scholar
Braczkowski, A., L. Watson, D. Coulson, J. Lucas, B. Peiser and M. Rossi. 2012. The diet of caracal, Caracal caracal, in two areas of the southern Cape, South Africa as determined by scat analysis. S. Afr. J. Wildl. Res. 42: 111–116.10.3957/056.042.0205Search in Google Scholar
Bunaian, F., A. Hatough, D. Ababneh, S. Mashaqbeh, M. Yousef and Z. Amr. 2001. The carnivores of the Northeastern Badia, Jordan. Turk. J. Zool. 25: 19–25.Search in Google Scholar
Casper, R.M., S.N. Jarman, B.E. Deagle, N.J. Gales and M.A. Hindell. 2007a. Detecting prey from DNA in predator scats: a comparison with morphological analysis, using Arctocephalus seals fed a known diet. J. Exp. Mar. Biol. Ecol. 347: 144–154.10.1016/j.jembe.2007.04.002Search in Google Scholar
Casper, R.M., S.N. Jarman, N.J. Gales and M.A. Hindell. 2007b. Combining DNA and morphological analyses of faecal samples improves insight into trophic interactions: a case study using a generalist predator. Mar. Biol. 152: 815–825.10.1007/s00227-007-0732-ySearch in Google Scholar
De Barba, M., J.R. Adams, C.S. Goldberg, C.R. Stansbury, D. Arias, R. Cisneros and L.P. Waits. 2014a. Molecular species identification for multiple carnivores. Conserv. Genet. Resour. 6: 821–824.10.1007/s12686-014-0257-xSearch in Google Scholar
De Barba, M., C. Miquel, F. Boyer, C. Mercier, D. Rioux, E. Coissac and P. Taberlet. 2014b. DNA metabarcoding multiplexing and validation of data accuracy for diet assessment: application to omnivorous diet. Mol. Ecol. Resour. 14: 306–323.10.1111/1755-0998.12188Search in Google Scholar PubMed
Etemad, E. 1985. Mammals of Iran, Vol. 2. Department of Environment publish, Tehran, Iran.Search in Google Scholar
Farhadinia, M., M. Beheshti and A. Sadeghi. 2006. Identification of cheetah habitats in Abbasabad Naein Reserve. Iranian Cheetah Society (ICS), 150 pp.Search in Google Scholar
Farhadinia, M.S., H. Akbari, M. Beheshti and A. Sadeghi. 2007. Ecology and status of the Caracal, Caracal caracal (Carnivora: Felidae), in the Abbasabad Naein Reserve, Iran. Zool. Mid. East. 41: 5–10.10.1080/09397140.2007.10638221Search in Google Scholar
Ghoddousi, A., T. Ghadirian and H. Fahimi. 2009. Status of caracal in Bahram’gur protected area, Iran. Cat News 50: 10–13.Search in Google Scholar
Grolbler, J.H. 1981. Feeding behaviour of the caracal Felis caracal Schreber 1776 in the Mountain Zebra National Park. S. Afr. J. Zool. 16: 259–262.10.1080/02541858.1981.11447764Search in Google Scholar
Harrison, D.L. and P.J. Bates. 1991. The mammals of Arabia, second edition. Harrison Zoological Museum, Sevenoaks, UK.Search in Google Scholar
Hassan-Beigi, Y., C. Fernandes, M. Farhadinia and S. Montazami. 2014. Phylogeny and genetic diversity of caracal in Iran. Zool. Mid. East 7: 6.Search in Google Scholar
Karanth, K. and M. Sunquist. 1995. Prey selection by tiger, leopard and dhole in tropical forests. J. Appl. Ecol. 64: 439–450.10.2307/5647Search in Google Scholar
Karanth, K.U., J.D. Nichols, N.S. Kumar, W.A. Link and J.E. Hines. 2004. Tigers and their prey: Predicting carnivore densities from prey abundance. PNAS 101: 4854–4858.10.1073/pnas.0306210101Search in Google Scholar PubMed PubMed Central
Kartzinel, T.R., P.A. Chen, T.C. Coverdale, D.L. Erickson, W.J. Kress, M.L. Kuzmina, D.I. Rubenstein, W. Wang and R.M. Pringle. 2015. DNA metabarcoding illuminates dietary niche partitioning by African large herbivores. PNAS 112: 8019–8024.10.1073/pnas.1503283112Search in Google Scholar PubMed PubMed Central
Klare, U., J.F. Kamler and D.W. Macdonald. 2011. A comparison and critique of different scat-analysis methods for determining carnivore diet. Mammal Rev. 41: 294–312.10.1111/j.1365-2907.2011.00183.xSearch in Google Scholar
Kocher, T.D., W.K. Thomas, A. Meyer, S.V. Edwards, S. Pääbo, F.X. Villablanca and A.C. Wilson. 1989. Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. PNAS 86: 6196–6200.10.1073/pnas.86.16.6196Search in Google Scholar PubMed PubMed Central
Krahn, M.M., D.P. Herman, C.O. Matkin, J.W. Durban, L. Barrett-Lennard, D.G. Burrows, M.E. Dahlheim, N. Black, R.G. LeDuc and P.R. Wade. 2007. Use of chemical tracers in assessing the diet and foraging regions of eastern North Pacific killer whales. Mar. Environ. Res. 63: 91–114.10.1016/j.marenvres.2006.07.002Search in Google Scholar PubMed
Litvaitis, J.A. 2000. Investigating food habits of terrestrial vertebrates. In: (L. Boitani and T.K. Fuller, eds.) Research techniques in animal ecology: controversies and consequences. Columbia University Press, New York. pp. 165–190.Search in Google Scholar
Mallon, D. and K. Budd. 2011. Regional Red List Status of Carnivores in the Arabian Peninsula. Cambridge, UK and Gland Switzerland, IUCN, and Sharjah, UAE: Environment and Protected Areas Authority.Search in Google Scholar
Melville, H.I.A.S., J.d.P. Bothma and M.G.L. Mills. 2004. Prey selection by caracal in the Kgalagadi Transfrontier Park. S. Afr. J. Wildl. Res. 34: 67–75.Search in Google Scholar
Mills, M.G.L. 1992. A comparison of methods used to study food habits of large African carnivores. In: (D.R. McCullough and R.H. Barrett, eds.) Wildlife 2001: Populations. Springer, Netherlands, Dordrecht. pp. 1112–1124.10.1007/978-94-011-2868-1_85Search in Google Scholar
Mills, L.S., M.E. Soule and D.F. Doak. 1993. The keystone-species concept in ecology and conservation. BioScience 43: 219–224.10.2307/1312122Search in Google Scholar
Mukherjee, S., S.P. Goyal, A.J.T. Johnsingh and M.R.P.L. Pitman. 2004. The importance of rodents in the diet of jungle cat (Felis chaus), caracal (Caracal caracal) and golden jackal (Canis aureus) in Sariska Tiger Reserve, Rajasthan, India. J. Zool. 262: 405–411.10.1017/S0952836903004783Search in Google Scholar
Mumma, M., J. Adams, C. Zieminski, T. Fuller, P.M. Shane and L. Waits. 2016. A comparison of morphological and molecular diet analyses of predator scats. J. Mammal. 97: 112–120.10.1093/jmammal/gyv160Search in Google Scholar
Palmer, R. and N. Fairall. 1988. Caracal and African wild cat diet in the Karoo National Park and the implications thereof for hyrax. S. Afr. J. Wildl. Res. 18: 30–34.Search in Google Scholar
Power, M.E., D. Tilman, J.A. Estes, B.A. Menge, W.J. Bond, L.S. Mills, G. Daily, J.C. Castilla, J. Lubchenco and R.T. Paine. 1996. Challenges in the quest for keystones. BioScience 46: 609–620.10.2307/1312990Search in Google Scholar
Pringle, J.A. and V.L. Pringle. 1978. Observations of the lynx Felis caracal in the Bedford district. S. Afr. J. Zool. 14: 1–4.10.1080/02541858.1979.11447639Search in Google Scholar
Shehzad, W., T.M. McCarthy, F. Pompanon, L. Purevjav, E. Coissac, T. Riaz and P. Taberlet. 2012a. Prey Preference of Snow Leopard (Panthera uncia) in South Gobi, Mongolia. PLoS One 7: e32104.10.1371/journal.pone.0032104Search in Google Scholar PubMed PubMed Central
Shehzad, W., T. Riaz, M.A. Nawaz, C. Miquel, C. Poillot, S.A. Shah, F. Pompanon, E. Coissac and P. Taberlet. 2012b. Carnivore diet analysis based on next-generation sequencing: application to the leopard cat (Prionailurus bengalensis) in Pakistan. Mol. Ecol. 21: 1951–1965.10.1111/j.1365-294X.2011.05424.xSearch in Google Scholar PubMed
Spaulding, R., P.R. Krausman and W.B. Ballard. 2000. Observer bias and analysis of gray wolf diets from scats. Wildl. Soc. Bull. 28: 947–950.Search in Google Scholar
Stuart, C.T. and G.C. Hickman. 1991. Prey of caracal Felis caracal in two areas of Cape Province, South Africa. J. Afr. Zool. 105: 373–381.Search in Google Scholar
Stuart, C. and M. Stuart. 2007. Diet of leopard and caracal in the northern United Arab Emirates and adjoining Oman territory. Cat News 46: 30–31.Search in Google Scholar
Sunquist, M., L. Rajaratnam and L. Ambu. 2007. Diet and habitat selection of the leopard cat (Prionailurus bengalensis borneoensis) in an agricultural landscape in Sabah, Malaysian Borneo. J. Trop. Ecol. 23: 209–217.10.1017/S0266467406003841Search in Google Scholar
Symondson, W.O.C. 2002. Molecular identification of prey in predator diets. Mol. Ecol. 11: 627–641.10.1046/j.1365-294X.2002.01471.xSearch in Google Scholar PubMed
Tollit, D.J., A.D. Schulze, A.W. Trites, P.F. Olesiuk, S.J. Crockford, T.S. Gelatt, R.R. Ream and K.M. Miller. 2009. Development and application of DNA techniques for validating and improving pinniped diet estimates. Ecol. Appl. 19: 889–905.10.1890/07-1701.1Search in Google Scholar PubMed
Van Heezik, Y. and P. Seddon. 1998. Range use and habitat use of an adult male caracal in northern Arabia. J. Arid. Environ. 40: 109–112.10.1006/jare.1998.0433Search in Google Scholar
Zeale, M.R., R.K. Butlin, G.L. Barker, D.C. Lees and G. Jones. 2011. Taxon-specific PCR for DNA barcoding arthropod prey in bat faeces. Mol. Ecol. Resour. 11: 236–244.10.1111/j.1755-0998.2010.02920.xSearch in Google Scholar PubMed
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