Abstract
Surfactant-stable keratinases with good properties are promising candidates for extensive applications in detergent industries. A novel fungal keratinase-producing strain, Gibberella intermedia CA3-1, is described in this study. The keratinase production medium was optimized and composed of 10 g L−1 of wool powder, 5 g L−1 of tryptone, 10 g L−1 of maltodextrin and 0.5 g L−1 of NaCl. Keratinase activity was increased up to 109 U mL−1 from 15 U mL−1 by culture optimization. The optimal reaction pH and temperature of the enzyme were 9.0 and 60°C, respectively. The keratinase activity could be improved by sodium dodecyl sulphate (SDS), and it remained stable in the presence of several surfactants and commercial detergents. G. intermedia keratinase was proved to completely remove blood stains from cotton cloth when combined with detergents. These findings indicate that this fungal keratinase is a promising catalyst for the application in detergent industry. To our knowledge, this is the first report on keratinase production by Gibberella genus.
Acknowledgements
This work was financially supported by the National High Technology Research and Program of the People's Republic of China (No. 2012AA022204C), and the Ministry of Education of the People's Republic of China (No. JUSRP51516).
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