Abstract
Hydroxyapatite (HA)-based biografts with selenium (Se) and silver (Ag) substitutions were synthesized using the sol-gel method. The synthesized HA-based biografts at various Se and Ag quantity ratios (wt%) were characterized via Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and energy dispersive X-Ray spectroscopy (EDX). Escherichia coli (JM103) and Gram-positive Staphylococcus aureus (ATCC29293) bacteria were used for the cell viability tests by performing the MTT assay. During antibacterial tests, it was determined that the synthesized biografts showed significant antimicrobial activity on E. coli and S. aureus; however, some materials were effective on Gram-negative E. coli, but had no effect on Gram-positive S. aureus. In vitro cell viability tests revealed that some of the synthesized biografts such as H30Ag10Se15 and H40Ag20Se10 provided the highest cell viability rates compared to those in the control group.
Author Statement
Research funding: Authors state no funding involved.
Conflict of interest: Authors state no conflict of interest.
Informed consent: Informed consent is not applicable. Ethical approval: The conducted research is not related to either human or animals use.
References
[1] Salgado AJ, Coutinho OP, Reis RL. Bone tissue engineering: state of the art and future trends. Macromol Biosci 2004;4:743–65.10.1002/mabi.200400026Search in Google Scholar PubMed
[2] Hench LL. Bioceramics. J Am Ceram Soc 1998;81:1705–28.10.1111/j.1151-2916.1998.tb02540.xSearch in Google Scholar
[3] Verret DJ, Ducic Y, Oxford L, Smith J. Hydroxyapatite cementin craniofacial reconstruction. Otolaryngol Head Neck Surg 2005;133:897–9.10.1016/j.otohns.2005.09.001Search in Google Scholar PubMed
[4] Zakharov NA, Polunina IA, Polunin KE, Rakitina NM, Kochetkova EI, Sokolova NP, et al. Calcium hydroxyapatite for medical applications. Inorg Mater 2004;40:641–8.10.1023/B:INMA.0000032000.83171.9fSearch in Google Scholar
[5] Ravaglioli A, Krajewski A, Portu G. Problems involved in assessing mechanical behaviour of bioceramics. In: Oonishi H, Aoki H, Sawai K, editors. Bioceramics, Proceed 1st Int Biocer, Vol. 1. Kyoto, Japan: Ishiyaku EuroAmerica; 1989:13–8.Search in Google Scholar
[6] Suchanek W, Yashima M, Kakihana M, Yoshimura M. Hydroxyapatite ceramics with selected sintering additives. Biomaterials 1997;18:923–33.10.1016/S0142-9612(97)00019-7Search in Google Scholar PubMed
[7] Kalita SJ, Bose S, Hosick HL, Bandyopadhyyay A. CaO-P2O5-Na2O-based sintering additives for hydroxyapatite (HAp) ceramics. Biomaterials 2004;25:2331–9.10.1016/j.biomaterials.2003.09.012Search in Google Scholar
[8] Georgiou G, Knowles JC. Glass reinforced hydroxyapapatite for hard tissue surgery-part 1: mechanical properties. Biomaterials 2001;22:2811–5.10.1016/S0142-9612(01)00025-4Search in Google Scholar
[9] Bernache-Assollant D, Ababou A, Champion E, Heughebaert M. Sintering of calcium phosphate hydroxyapatite Ca10(PO4)6(OH)2 I. Calcination and particle growth. J Eur Ceram Soc 2003;23:229–41.10.1016/S0955-2219(02)00186-3Search in Google Scholar
[10] Tran PA, Sarin L, Hurt RH, Webster TJ. Titanium surfaces with adherent selenium nanoclusters as anovelanti cancer orthopedicmaterial. J Biomed Mater Res A 2009;93:1417–28.Search in Google Scholar
[11] Rodríguez-Valencia C, López-Álvarez M, Cochón-Cores B, Pereiro I, Serra J, González P. Novel selenium-doped hydroxyapatite coatings for biomedical applications. J Biomed Mater Res A 2013;101A:853–61.10.1002/jbm.a.34387Search in Google Scholar
[12] Tancred DC, Mccormack BAO, Carr AJ. A quantitative study of the sintering and mechanical properties of hydroxyapatite/phosphate glass composites. Biomaterials 1998;19:1735–43.10.1016/S0142-9612(98)00082-9Search in Google Scholar PubMed
[13] Ma J, Wang YH, Zhou L, Zhang SM. Preparation and characterization of selenite substituted hydroxyapatite. Mater Sci Eng C 2013;33:440–5.10.1016/j.msec.2012.09.011Search in Google Scholar PubMed
[14] Chen J, Li S, Luo J, Wang R, Ding W. Enhancement of the antibacterial activity of silver nanoparticles against phytopathogenic bacterium ralstonia solanacearum by stabilization. J Nanomater 2016;2016:Article ID 7135852, 15 pages. http://dx.doi.org/10.1155/2016/7135852.10.1155/2016/7135852Search in Google Scholar
[15] Le Ouay B, Stellacci F. Antibacterial activity of silver nanoparticles: a surface science insight. Nano Today 2015;10:339–54.10.1016/j.nantod.2015.04.002Search in Google Scholar
[16] Stevanovic M, Filipovic N, Djurdjevic J, Lukic M, Milenkovic M, Boccaccini A. 45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: processing, evaluation and antibacterial activity. Colloids Surf B Biointerfaces 2015;132:208–15.10.1016/j.colsurfb.2015.05.024Search in Google Scholar PubMed
[17] Darouiche RO. Current concepts: treatment of ınfections associated with surgical ımplants. N Engl J Med 2004;350:1422–9.10.1056/NEJMra035415Search in Google Scholar PubMed
[18] de Sanctis J, Teixeira L, van Duin D, Odio C, Hall G, Tomford JW, et al. Complex prosthetic joint infections due to carbapenemase-producing Klebsiella pneumoniae: a unique challenge in the era of untreatable infections. Int J Infect Dis 2014;25:73–8.10.1016/j.ijid.2014.01.028Search in Google Scholar PubMed PubMed Central
[19] Oldfield E, Feng X. Resistance-resistant antibiotics. Trends Pharmacol Sci 2014;35:664–74.10.1016/j.tips.2014.10.007Search in Google Scholar PubMed PubMed Central
[20] Stevanovic M, Uskokovic V, Filipovic M, Skapin SD, Uskokovic D. Composite PLGA/AgNpPGA/AscH nanospheres with combined osteoinductive, antioxidative and antimicrobial activities. ACS Appl Mater Interfaces 2013;5:9034–42.10.1021/am402237gSearch in Google Scholar PubMed PubMed Central
[21] Zeng H, Cao JJ, Combes GF. Selenium in bone health: roles in antioxidant protection and cell proliferation. Nutrients 2013;5:97–110.10.3390/nu5010097Search in Google Scholar PubMed PubMed Central
[22] Moreno-Reyes R, Egrise D, Neve J, Pasteels JL, Schoutens A. Selenium deficiency-induced growth retardation is associated with an impaired bone metabolism and osteopenia. J Bone Miner Res 2001;16:1556–63.10.1359/jbmr.2001.16.8.1556Search in Google Scholar PubMed
[23] Tran PA, Webster TJ. Selenium nanoparticles inhibit Staphylococcus aureus growth. Int J Nanomed 2011;6:1553–8.10.2147/IJN.S21729Search in Google Scholar PubMed PubMed Central
[24] Prabakan K, Balamurugan A, Rajeswarı S. Development of calcium phosphate based apatite from hen’s eggshell. Bull Mater Sci 2005;28-2:115–9.10.1007/BF02704229Search in Google Scholar
[25] Cai S, Zhang WJ, Xu GH, Li JY, Wang DM, Jiang W. Microstructural characteristics and crystallization of CaO-P2O5-Na2O-ZnO glass Ce-ramics prepared by sol-gel method. J Non-Crystalline Solids 2009;355:273–9.10.1016/j.jnoncrysol.2008.11.008Search in Google Scholar
[26] Tran PA, Webster TJ. Antimicrobial selenium nanoparticle coatings on polymeric medical devices. Nanotechnology 2013;24:155101.10.1088/0957-4484/24/15/155101Search in Google Scholar PubMed
[27] Mittal AK, Kumar S, Banerjee UC. Quercetin and gallic acid mediated synthesis of bimetallic (silver and selenium) nanoparticles and their antitumor and antimicrobial potential. J Colloid Interface Sci 2014;431:194–9.10.1016/j.jcis.2014.06.030Search in Google Scholar PubMed
[28] Ahmad Barudin NH, Sreekantan S, Ong MT, Lai CW. Synthesis, characterization and comparative study of nano-Ag–TiO2 against Gram-positive and Gram-negative bacteria under fluorescent light. Food Control 2014;46:480–7.10.1016/j.foodcont.2014.05.046Search in Google Scholar
[29] Li JX, Wang J, Shen LR, Xu ZJ, Li P, Wan GJ, et al. The influence of polyethylene terepthylene surface modified by silver ion implantation on bacterial adhesion behavior. Surf Coat Technol 2007;201:8155–9.10.1016/j.surfcoat.2006.02.069Search in Google Scholar
[30] Chen W, Liu Y, Courtney HS, Bettenga M, Agrawal CM, Bumgardner JD, et al. Invitro antibacterial and biological properties of magnetronco-sputtered silver-containing hydroxyapatite coating. Biomaterials 2006;27:5512–7.10.1016/j.biomaterials.2006.07.003Search in Google Scholar PubMed
[31] Simon V, Albon C, Simon S. Silver release from hydroxyapatite self-assembling calcium–phosphate glasses. J Non-Cryst Solids 2008;354:1751–5.10.1016/j.jnoncrysol.2007.08.063Search in Google Scholar
[32] Rai M, Yadav A, Gade A. Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv 2009;27:76–83.10.1016/j.biotechadv.2008.09.002Search in Google Scholar PubMed
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