Skip to main content

Advertisement

Log in

Genetic relationships of Chinese prickly ash as revealed by ISSR markers

  • Published:
Biologia Aims and scope Submit manuscript

Abstract

Chinese prickly ash, belonging to the genus Zanthoxylum L., has been one of the most important commercially-exploited plants for its alimentary, industrial and medicinal applications. However, the breeding and promotion of Chinese prickly ash have been severely restricted due to its confusing classification. Therefore, we assessed genetic diversity and phylogenetic relationship among 45 Chinese prickly ash samples collected from 6 main cultivated regions using 11 ISSR primers. These eleven selected primers generated a total of 102 scorable bands ranging from 150 to 2000 bp, corresponding to an average of approximately 9.3 bands per primer. The percentage of polymorphic loci for all samples ranged from 75% to 100%, with an average of 84.3%. The genetic similarity coefficient across all samples varied from 0.460 to 0.919. Remarkably, UPGMA analysis showed that 45 samples were divided into six clusters with a genetic similarity of 0.7. The closest genetic relationship was observed between Dahongpao collected from Qin’an and Tianshui, and the greatest genetic divergence was found between Dahongpao collected from Hengshui and Jiuyeqing collected from Jiangjin. It could serve as a basis for identifying Chinese prickly ash cultivars, breeding new cultivars and protecting the Chinese prickly ash resources in main regions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Amar M.H., Biswas M.K., Zhang Z.W. & Guo W.W. 2011. Exploitation of SSR, SRAP and CAPS-SNP markers for genetic diversity of Citrus germplasm collection. Sci. Hort. 128: 220–227.

    Article  CAS  Google Scholar 

  • Bai J.J., Wei A.Z., Wang J. & Zhang Y.Z. 2009. Optimization of an ISSR analysis system of apricot of kernel-consuming based on orthogonal design. Mol. Plant Breed. 7: 1237–1244.

    CAS  Google Scholar 

  • Bowers W.S., Ortego F., You X.Q. & Evans P.H. 1993. Insect repellents from the Chinese prickly ash Zanthoxylum bungeanum. J. Nat. Prod. 56: 935–938.

    Article  CAS  Google Scholar 

  • Bryant B.P. & Mezine I. 1999. Alkylamides that produce tingling paresthesia activate tactile and thermal trigeminal neurons. Brain Res. 842: 452–460.

    Article  CAS  Google Scholar 

  • Chang C.E. & Hartley T.G. 1993. Rutaceae in Flora of Taiwan. 2nd edition. Editorial Committee of the Flora of Taiwan, Taip ei. Taiwan. 3: 44–510.

    Google Scholar 

  • Chen J.J., Lin Y.H., Day S.H., Hwangb T.L. & Chen I.S. 2011. New benzenoids and anti-inflammatory constituents from Zanthoxylum nitidum. Food Chem. 125: 282–287.

    Article  CAS  Google Scholar 

  • Cheng M.J., Lee K.H., Tsai I.L. & Chen I.S. 2005 Two new sesquiterpenoids and anti-HIV principles from the root bark of Zanthoxylum ailanthoides. Bioorg. Med. Chem. 13: 5915–5920.

    Article  CAS  Google Scholar 

  • Deng F., Mo J.L. & Chen H.A. 2009 Identification of Exocarpium Citri Grandis by inter-simple sequence repeat markers. J. Guangdong Pharm. Coll. 25: 455–458.

    CAS  Google Scholar 

  • Deng H.P., Xu J., Chen F. & Song Q.Z. 2008 Morphological and molecular identification on genetic diversity of Zanthoxylum armatum var. novemfolius. Acta. Bot. Boreal-Occident. Sin. 28: 2103–2109.

    CAS  Google Scholar 

  • Doh E.J., Yang N.J., Ji M.G., Lee G.S., Ju Y.S., Lee J.H., Ko B.S. & Oh S.E. 2011. Discrimination of Kalopanax pictus from its varieties and other ‘Kalopanacis Cortex’ plants by multiplex polymerase chain reaction (PCR). Genes. Genom. 33: 711–719.

    Article  Google Scholar 

  • Dube S., Kumar A. & Tripathi S.C. 1990. Antifungal and insect-repellent activity of essential oil of Zanthoxylum alatum. Ann. Bot. 65: 457–459.

    Article  Google Scholar 

  • Epple G., Bryant B.P., Mezine I. & Lewis S. 2001. Zanthoxylum piperitum, an Asian spice, inhibits food intake in rats. J. Chem. Ecol. 27: 1627–1640.

    Article  CAS  Google Scholar 

  • Esselman E.J., Jiangqiang L., Crawford D.J., Windus J.L. & Wolfe A.D. 1999. Clonal diversity in the rare Calamagrostis porteri ssp. insperata (Poaceae): comparative results for allozymes and random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) markers. Mol. Ecol. 8: 443–451.

    Article  CAS  Google Scholar 

  • Gemas V.J.V., Almadanim M.C., Tenreiro R., Martins A. & Fevereiro P. 2004. Genetic diversity in the Olive tree (Olea europaea L. subsp. europaea) cultivated in Portugal revealed by RAPD and ISSR markers. Genet. Resour. Crop. Ev. 51: 501–511.

    Article  CAS  Google Scholar 

  • Godwin I.D., Aitken E.A.B. & Smith L.W. 1997. Application of inter simple sequence repeat (ISSR) markers to plant genetics. Electrophoresis 18: 1524–1528.

    Article  CAS  Google Scholar 

  • He W., Puyvelde L.V., Kimpe N.D., Verbruggen L., Anthonissen K., Flaas M.V.D., Bosselaers J., Mathenge S.G. & Mudida F.P. 2002. Chemical constituents and biological activities of Zanthoxylum usambarense. Phytother. Res. 16: 66–70.

    Article  CAS  Google Scholar 

  • Henry R.J. 2001. Plant genotyping: the DNA fingerprinting of plants. Wallingford: CABI Publishing.

    Book  Google Scholar 

  • Huang C. 1997. Reipublicae popularis sinicae. In: Delectis Florae Reipublicae Popularis Sinicae agendae academiae sinicae edita. Beijing: Science Press. 43: 8–10.

    Google Scholar 

  • Huang S., Zhao L., Zhou X.L., Ying M., Wang C.J. & Weng J. 2012. New alkylamides from pericarps of Zanthoxylum bungeanum. Chinese. Chem. Lett. 23: 1247–1250.

    Article  CAS  Google Scholar 

  • Jacobson M. & Crosby D.G. 1971. Naturally occurring insecticides. New York: Marcel Decker.

    Google Scholar 

  • Joshi S.P., Gupta V.S., Aggarwal R.K., Ranjekar P.K. & Brar D.S. 2000. Genetic diversity and phylogenetic relationship as revealed by inter simple sequence repeat (ISSR) polymorphism in the genus Oryza. Theor. Appl. Genet. 100: 1311–1320.

    Article  CAS  Google Scholar 

  • Liu Y.G., Chen S.L., Li J. & Li B.F. 2006. Genetic diversity in three Japanese flounder (Paralichthys olivaceus) populations revealed by ISSR markers. Aquaculture 255: 565–572.

    Article  CAS  Google Scholar 

  • Min B.K., Hyun D.G., Jeong S.Y., Kim Y.H., Ma E.S. and Woo M.H. 2011. A new cytotoxic coumarin, 7-[(E)-3, 7/-dimethyl-6/-oxo-2/, 7/-octadienyl] oxy coumarin, from the leaves of Zanthoxylum schinifolium. Arch. Pharm. Res. 34: 723–726.

    Article  CAS  Google Scholar 

  • Murray M.G. & Thompson W.F. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic. Acids. Res. 8: 4321–4326.

    Article  CAS  Google Scholar 

  • Patino L.O.J., Prieto R.J.A. & Cuca S.L.E. 2008. Bioactive compounds in phytomedicine: Zanthoxylum genus as potential source of bioactive compounds, pp. 185–218. In: Rasooli I. (ed.), In Tech, Rijeka Croatia.

    Google Scholar 

  • Peng Y., Su Z.X. & Zhang S.L. 2008. Detecting genetic diversity by morphological characteristics of leaves and ISSR markers in Citrus grandis. J. Northwest A&F University (Nat. Sci. Ed.). 4: 104–110.

    Google Scholar 

  • Pfander H.J. & Frohne D. 1987. Szechuan-Pfeffer. Die Früchte von Zanthoxylum piperitum DC.(Rutaceae). Dtsch. Apoth. Ztg. 127: 2381–2384.

    Google Scholar 

  • Rana T.S., Narzary D. & Ohri D. 2010. Genetic diversity and relationships among some wild and cultivated species of Chenopodium L.(Amaranthaceae) using RAPD and DAMD methods. Curr. Sci. 98: 840–846.

    CAS  Google Scholar 

  • Seidemann J. 2005. World spice plants: Economic Usage, Botany, Taxonomy. Springer-Verlag.

    Google Scholar 

  • Song Z.Q., Li X.F., Wang H.G. & Wang J.H. 2010. Genetic diversity and population structure of Salvia miltiorrhiza Bge in China revealed by ISSR and SRAP. Genetica 138: 241–249.

    Article  CAS  Google Scholar 

  • Svobodová E., Dvořáková Z., Čepková P.H., Viehmannová I., Havlíčková L., Cusimamani E.F., Russoc D. & Zelad G.M. 2013. Genetic diversity of yacon (Smallanthus sonchifolius (Poepp. & Endl.) H. Robinson) and its wild relatives as revealed by ISSR markers. Biochem. Syst. Ecol. 50: 383–389.

    Article  Google Scholar 

  • Tantapakul C., Phakhodee W., Ritthiwigrom T., Yossatheral K., Deachathail S. & Laphookhieo S. 2012. Antibacterial compounds from Zanthoxylum rhetsa. Arch. Pharm. Res. 35: 1139–1142.

    Article  CAS  Google Scholar 

  • Wang S., Xie J.C., Yang W. & Sun B.G. 2011. Preparative separation and purification of alkylamides from Zanthoxylum bungeanum Maxim by high-speed counter-current chromatography. J. Liq. Chromatogr. R. T. 34: 2640–2652.

    Article  CAS  Google Scholar 

  • Wei S.G., Zhang H.H., Wang Y.Q., Wang L., Li X.Y., Wang Y.H., Zhang H.Q., Xu X. & Shi Y.H. 2011. Ultrasonic nebulization extraction-heating gas flow transfer-headspace single drop microextraction of essential oil from pericarp of Zanthoxylum bungeanum Maxim. J. Chromatogr. A. 1218: 4599–4605.

    Article  CAS  Google Scholar 

  • Yilmaz K.U., Paydas-Kargi S., Dogan Y. & Kafkas S. 2012. Genetic diversity analysis based on ISSR, RAPD and SSR among Turkish apricot germplasms in Iran Caucasian ecogeographical group. Sci. Hortic. 138: 138–143.

    Article  CAS  Google Scholar 

  • Zheng H.X., Li Z.Q., Xue H.D., Wang D.W. & Sun Y. 2011. RAPD Analysis of the germplasm resources of Zanthoxylum bungeanum. J. Northwest For Univ. 26: 96–100.

    Google Scholar 

  • Zhong F. L., Shi W. S. & Pan D. M. 2010. ISSR analysis of Fujian pummelo germplasms. Chin. J. Trop. Crops. 31: 1964–1968.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Anzhi Wei.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Feng, S., Yang, T., Li, X. et al. Genetic relationships of Chinese prickly ash as revealed by ISSR markers. Biologia 70, 45–51 (2015). https://doi.org/10.1515/biolog-2015-0005

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1515/biolog-2015-0005

Key words

Navigation