Abstract
Babesia bovis is an important pathogen of bovine babesiosis and causes serious constraints on the health and productivity of domestic cattle in the tropical and subtropical areas of the world. Aiming to clarify the prevalence of B. bovis in China, a total of 2,364 cattle serum samples were randomly collected from 45 different areas of 17 provinces in China. Antibodies against B. bovis were tested by enzyme-linked immunosorbent assay (ELISA) using a recombinant C-terminal antigen of B. bovis rhoptry-associated protein-1 (RAP-1) to evaluate the prevalence of B. bovis. The results showed that the parasite was present in all of the investigated 17 provinces. The positive rate was from 6.40 to 47.27%, and the mean rate was 24.92%. These survey data will provide important information for designing control strategies for bovine babesiosis in China.
References
Bai Q., Liu G.Y., Zhang L., Zhou J.Y. 1990. Discovery and isolation of Babesia ovata in China. Chinese Journal of Veterinary Science and Technology, (12), 2-5Search in Google Scholar
Bock R., Jackson L., de Vos A., Jorgensen W. 2004. Babesiosis of cattle. Parasitology, 129, s247-s269. DOI: 10.1017/S00311 82004005190Search in Google Scholar
Boonchit S., Xuan X.N., Yokoyama N., Goff W.L., Wagner G., Igarashi I. 2002. Evaluation of an enzyme-linked immunosorbent assay with recombinant rhoptry-associated protein-1 antigen of Babesia bovis for the detection of specific antibodies in cattle. Journal of Clinical Microbiology, 40, 3771-3775. DOI: 10.1128/JCM.40.10.3771-3775.200210.1128/JCM.40.10.3771-3775.2002Search in Google Scholar
Boonchit S., Xuan X.N., Yokoyama N., Goff W.L., Waghela S.D., Wagner G., Igarashi I. 2004. Improved enzyme-linked immunosorbent assay using C-terminal truncated recombinant antigens of Babesia bovis rhoptry-associated protein-1 for detection of specific antibodies. Journal of Clinical Microbiology, 42, 1601-1604. DOI: 10.1128/JCM.42.4.1601-1604. 2004Search in Google Scholar
Callow L.L. 1984. Piroplasms. In Animal Health in Australia, Protozoal and Rickettsial Diseases, vol. 5. Animal Health in Australia, pp. 121-160. Canberra, Australian Bureau of Animal Health, AGPS Search in Google Scholar
Chen Z., Yang X.J., Bu F.J., Yang X.H., Yang X.L., Liu J.Z. 2010. Ticks (Acari: Ixodoidea: Argasidae, Ixodidae) of China. Experimental and Applied Acarology, 51, 393-40. DOI: 10.1007/s10493-010-9335-210.1007/s10493-010-9335-2Search in Google Scholar
De Echaide S.T., Echaide I.E., Gaido A.B., Mangold A.J., Lugaresi C.I., Vanzini V.R., Guglielnone A.A. 1995. Evaluation of an enzyme-linked immunosorbent assay kit to detect Babesia bovis antibodies in cattle. Preventive Veterinary Medicine, 24, 277-283. DOI: 10.1016/0167-5877(95)00485-F 10.1016/0167-5877(95)00485-FSearch in Google Scholar
Dominguez M., Zabal O., Wilkowsky S., Echaide I., Torioni D.E., Asenzo G., Rodriguez A., Zamorano P., Farber M., Suarez C., Florin-christensen M. 2004. Use of a monoclonal antibody against Babesia bovis merozoite surface antigen-2c for the development of a competitive ELISA test. New York Academy of Sciences, 1026, 165-170. DOI: 10.1196/annals.1307.02510.1196/annals.1307.025Search in Google Scholar
Goff W.L., McElwain T.F., Suarez C.E. ,Johnson W.C., Brown W.C., Norimine J., Knowles D.P. 2003. Competitive enzyme-linked immunosorbent assay based on a rhoptry-associated protein 1 epitope specifically identifies Babesia bovis-infected cattle. Clinical and Diagnostic Laboratory Immunology, 10, 1, 38-43. DOI: 10.1128/CDLI.10.1.38-43.200310.1128/CDLI.10.1.38-43.2003Search in Google Scholar
Jian Z.J., Ma S.Z., Yuan J.L., Shen J.Y., Lu W.,Sun Q.Z., Miao Z.Q. 2011a Establishment of A GST-MSA-2C Fusion Protein Indirect ELISA Assay for Serodiagnosis of Babesia bovis. Xinjiang Agricultural Sciences, 48(1), 139-142Search in Google Scholar
Jian Z.J., Ma S.Z., Sun Q.Z., Shen J.Y., Lu W., Miao Z.Q. 2011b. Epidemiologic Survey for Detection of Babesia bovis-Infected Cattle in XinJiang by an Improved MSA-2c Indirect ELISA Assay. Xinjiang Agricultural Sciences, 48, 2129-2133Search in Google Scholar
Li A.Y., Liu A.H., Wang J.M., Zhu H., Ma M.L., Ren Q.Y., Guan G.Q., Luo J.X. 2013. Cloning and expression of C terminal for rhoptry-associated protein 1 of Babesia bovis. Chinese Veterinary Science, 43, 588-593Search in Google Scholar
Li A.Y., Liu A.H., Wang J.M., Zhu H., Ma M.L., Ren Q.Y., Guan G.Q., Luo J.X. 2014. Development of ELISA for diagnosis of babesiosis in cattle. Chinese Veterinary Science, 44(01), 62-67Search in Google Scholar
Liu Z.L., Zhao J.L., Ma L.H., Yao B.A. 1997. Babesia orientalis sp. nov. parasitized in buffalo Bubalus babalis in China (Piroplasmida: Babesiidae). Acta Veterinaria Et Zootechnica Sinica, 28, 84-89Search in Google Scholar
Lu W.S., Yin H., Luo J.X., Lu W.X., Zhang Q.C., Dou H.F. 1992a. Discovery of Babesia major in Xinjiang. In: Symposium of the 3rd Meeting of Chinese Society of Veterinary Parasitology, p. 220Search in Google Scholar
Lu W.X., Lu W.S., Zhang Q.C., Dou H.F., Yin H.,Luo J.X. 1992b. Survey of the species of tick-borne haemoprotozoan of cattle and feature in GanSu Province. Chinese Journal of Veterinary Sciences and Technology, 22, 13-15Search in Google Scholar
Lu W.X., Lu W.S., Zhang Q.C., Luo J.X., Yin H., Dou H.F. 1995. Survey of the species of tick-borne haemoprotozoan of cattle and feature in China. Chinese Journal of Veterinary Sciences and Technology, 25, 13-16Search in Google Scholar
Luo J.X., Yin H., Liu Z.J., Yang D.Y., Guan G.Q., Liu A.H., Ma M.L., Dang Z.S., Lu B.Y., Sun C.Q., Bai Q, Lu W.S., Chen P.Y. 2005. Molecular phylogenetic studies on an unnamed bovine Babesia sp. based on small subunit ribosomal RNA gene sequences. Veterinary Parasitology, 133, 1-6. DOI: 10.1016/ j.vetpar.2005.02.01410.1016/j.vetpar.2005.02.014Search in Google Scholar
Molloy J.B., Bowles P.M., Bock R.E., Turton J.A., Katsande T.C., Katende J.M., Mabikacheche L.G., Waldron S.J., Blight G.W., Dalgliesh R.J. 1998. Evaluation of an ELISA for detection of antibodies to Babesia bovis in cattle in Australia and Zimbabwe. Preventive Veterinary Medicine, 33, 59-67. DOI: 10.1016/S0167-5877(97)00063-910.1016/S0167-5877(97)00063-9Search in Google Scholar
OIE. 2009. Bovine babesiosis. In: Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, CHAPTER 2. 4. 2. (www.oie.int) Search in Google Scholar
Waltisbuhl D.J., Goodger B.V., Wright I.G., Commins M.A.,Mahoney D.F. 1987. An enzyme linked immunosorbent assay to diagnose Babesia bovis infection in cattle. Parasitology Research, 73, 126-131. DOI: 10.1007/BF0053646810.1007/BF00536468Search in Google Scholar PubMed
Wang C.J. 1993. Bovine piroplasmosis. In: The diseases of Domestic Animals in China, edited by Division of Veterinary and Animal Husbandry, Minstry of Agriculture, Academic Press, 381-390Search in Google Scholar
Yang P., Wang X.W. 1964. Investigation on the haemoprotozoans in Guizhou Province. In: Symposium of the 1st National Meeting on Research of Veterinary Parasitology. pp. 351-355 Search in Google Scholar
Yin H., Lu W.S., Luo J.X. 1997. Babesiosis in China. Tropical animal health and production, 29, 11S-15S. DOI: 10.1007/ BF02632908 10.1007/BF02632908Search in Google Scholar PubMed
W. Stefański Institute of Parasitology, PAS