Abstract
Previous taxonomic studies reported that nine species of Martensia (i.e., Martensia albida, Martensia australis, Martensia bibarii, Martensia elegans, Martensia flammifolia, Martensia fragilis, Martensia jejuensis, Martensia projecta, and Martensia palmata) occur on the Korean coast. Our recent Martensia collections from various localities in Korea contain many specimens with a huge range of morphological variation and are difficult to classify into the named species. In this study, we analyzed DNA sequences of rbcL and COI genes to access their phylogenetic relationships and to verify taxonomic status based on morphological observations. The molecular analyses revealed that only two species of Martensia occur in Korea (M. albida and M. jejuensis). Martensia albida is characterized by having entire margins of blades with a single band of networks, whereas M. jejuensis is distinguished from the other species by possessing projections along the margins of the networks and blades. Both molecular analyses and morphological observations revealed that M. flammifolia is a member of the genus Nitophyllum. Some features (e.g., thallus habit, branching patterns, blade morphology, positions of reproductive structures) are not reliable for species identification. Our results indicate that the rbcL and the COI gene have sufficient resolution for delimitation at both species and generic levels in the subfamily Nitophylloideae.
About the authors
Jeong Chan Kang is a postdoctoral researcher at the Department of Biology, Jeju National University, Korea. He specializes in the family Delesseriaceae and is interested in the relationship between the molecular phylogeny and anatomical morphology.
Mi Yeon Yang is a PhD student at the Department of Biology, Jeju National University, Korea. She works on the diversity and phylogeny of other Gigartinales from Korea by using morphological and molecular analyses.
Showe-Mei Lin is a Professor at the Institute of Marine Biology, National Taiwan Ocean University. She specializes in the systematics of Delesseriaceae. She is interested in the molecular phylogeny, systematics, and cystocarp development of red algae, the biogeography of seaweeds, and the marine benthic macro-algal flora of Taiwan.
Myung Sook Kim is a Professor of Biology at Jeju National University, Jeju, Korea. She was awarded a PhD in Algal Systematics by the Seoul National University, Korea, for work on taxonomic revision of Polysiphonia and studied Systematics in Rhodophyta for over 15 years, especially in the family Rhodomelaceae. More recent research has concentrated on establishing a DNA barcode database for Korean seaweeds to identify species and genus correctly.
Acknowledgments
This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR No. 2014-0655 for collecting samples, and 1834-302 for molecular analyses).
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