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南極鳥類の赤血球における炭化水素代謝:IP5, 2,3-DPG 量とニワトリのヘキソキナーゼに対する効果
https://doi.org/10.15094/00009031
https://doi.org/10.15094/0000903155af3024-effc-4243-9e0f-180810235771
名前 / ファイル | ライセンス | アクション |
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KJ00000140393 (237.7 kB)
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Item type | 紀要論文(ELS) / Departmental Bulletin Paper(1) | |||||
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公開日 | 1998-03-01 | |||||
タイトル | ||||||
タイトル | 南極鳥類の赤血球における炭化水素代謝:IP5, 2,3-DPG 量とニワトリのヘキソキナーゼに対する効果 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Carbohydrate metabolism in Antarctic birds' erythrocytes: Levels of IP5 and 2,3-DPG and their effect on chicken hexokinase activity | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.15094/00009031 | |||||
ID登録タイプ | JaLC | |||||
ページ属性 | ||||||
内容記述タイプ | Other | |||||
内容記述 | P(論文) | |||||
論文名よみ | ||||||
その他のタイトル | ナンキョク チョウルイ ノ セッケッキュウ 二 タンカスイソ タイシャ:IP5, 2,3-DPG リョウトニワトリノ ヘキソキナーゼ ニタイスル コウカ | |||||
著者名(英) |
Rodrigues, Edson
× Rodrigues, Edson× Rosa, Rubens× Ocampos, Dario× Rosa, Claudete D.× Bacila, Metry |
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著者所属(英) | ||||||
en | ||||||
Faculdade de Ciencias Farmaceuticas,Universidade Sao Francisco | ||||||
著者所属(英) | ||||||
en | ||||||
Departamento de Medicina, Pontificia Universidade Catolica do Parana | ||||||
著者所属(英) | ||||||
en | ||||||
Faculdade de Zoologia e Tecnoloigia de Alimentos, Universidade de Sao Paulo | ||||||
著者所属(英) | ||||||
en | ||||||
Faculdade de Odontologia, Universidade Estadual Paulista-UINESP | ||||||
著者所属(英) | ||||||
en | ||||||
Laboratorio de Piscicultura,Universidade Federal do Parana | ||||||
抄録(日) | ||||||
内容記述タイプ | Other | |||||
内容記述 | 本文は鳥類赤血球中のグルコース代謝における, 有機燐酸代謝産物である, イノシトール5リン酸(IP5), 2,3ジホスホグリセリン酸(2,3-DPG)のもつ意味を知ることを目的とするものである。使用したヘキソキナーゼは, セファローズ-N-アミノヘキサノイルグルコサミンを用いたアフィニティクロマトグラフィを用いて精製した。IP5のヘキソキナーゼ抑制作用は, Mg^<2+>に対して競争的であるが, グルコース, MgATP^<2->に対しては競争的ではない。一方, ヘキソキナーゼの活性はATP^<4->によって抑制される。赤血球中のMg^<2+>値(2.76μmol/ml)は, 赤血球標準懸濁液中のATP, IP5の総量に比べて低い。 | |||||
抄録(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | The aim of the present study is to show the possible implication of organic phosphate metabolites=namely inositol pentaphosphate (IP5) and 2,3-bisphosphoglycerate (2,3-DPG)=in the glucose metabolism displayed by birds erythrocytes. For this purpose, a preparation of hexokinase (HK) has been purified from chicken erythrocytes (RBC) by affinity chromatography in Sepharose-N-aminohexanoyl glucosamine and used for kinetic experiments. It has been shown that IP5 is a competitive inhibitor of HK in regard to Mg^<2+>, but not in regard to glucose and MgATP^<2->. On the other hand, this preparation of HK is also competitively inhibited by ATP^<4->. It has been found that the total intracellular content of Mg^<2+> (2.76μmol/ml) is lower than the total value for the content of ATP and IP5 found in a standard packed red blood cell suspension. | |||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00181831 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00857289 | |||||
書誌情報 |
南極資料 en : Antarctic Record 巻 42, 号 1, p. 101-110, 発行日 1998-03 |
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国際十進分類法 | ||||||
主題Scheme | UDC | |||||
主題 | 591.5 |