The present research work was undertaken to assess the effect of gibberellic acid (GA₃) applied exogenously to the Safflower plant for the study of pollen behavior. A cytological characterization in safflower from meiosis to pollen formation revealed that whereas meiosis was recorded to be normal in control plants with normal microsporogenesis, GA₃-treated plants possessed disturbed tetrad configurations of pollen. GA₃ caused deductions in pollen yield and increased the formation of abnormal meiotic products. Our study elucidates that the microspore sacs, instead of having normal four microspores, possessed 1 (monad), 2 (dyads), 3 (triads) and even up to 5 (polyads) microspores, which may produce unreduced gametes at later stages. A significant variation in the degree of pollen mortality was also displayed amongst these pollens. Another interesting feature noticed during the study was the fusion among tetrads due to wall dissolution.