| The effects of green tea (Camellia sinensis) flower extract on melanin synthesis in B16-F10 melanoma cells |
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Chanuri-Yashara Dissanayake1, Hae-Hee Moon1, Kyeong-Mi Yang2, Younjae Lee1, Chang-Hoon Han1 |
1College of Veterinary Medicine, Jeju National University
2Dahrum International Co., Ltd., Jeju National University Medium & Small Business Center |
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| Abstract |
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The present study observed the effects of a green tea (Camellia sinensis) flower extract (GTFE) on melanin synthesis in B16-F10 melanoma cells. GTFE exhibited antioxidant activity on 2,2-diphenyl-1-picrylhydrazyl and inhibited mushroom tyrosinase activity in a dose-dependent manner. Furthermore, GTFE significantly diminished ${alpha}-melanocyte$ stimulating hormone (${alpha}-MSH$) stimulated cellular melanin content and tyrosinase activity throughout the concentration range evaluated. Based on RNA sequencing analysis, differential gene expression patterns observed in ${alpha}-MSH$ stimulated B16-F10 melanoma cells were normalized by the addition of GTFE. In particular, the expression levels of melanoregulin and tyrosinase genes which are key regulating genes in melanin synthesis were up-regulated by 3.5 and 3 fold respectively by ${alpha}-MSH$, and were normalized to control levels by the addition of GTFE. The results suggest that GTFE inhibits melanin synthesis in ${alpha}-MSH$ stimulated B16-F10 melanoma cells by normalizing expression of genes that are essential for melanin synthesis. Overall, the results suggest that GTFE could be applied in the development of a whitening agent for the treatment of dermal hyperpigmentation. |
| Key Words:
B16-F10 melanoma cells, RNA sequencing analysis, antioxidant effect, green tea flower, melanin synthesis inhibition, tyrosinase activity inhibition |
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