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Gene delivery into primary T cells

Overview and characterization of a transgenic model for efficient adenoviral transduction

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Abstract

Technologies for transfer of exogenous genes into primary T cells have been limited until recently. The introduction of new approaches for gene transfer viadifferent viral vectors has expan ded the options for genetic manipulation of primary T cells and has provided powerful tools for studies of T cell activation and differentiation. We provide a brief overview of the systems currently available and contranst the advantages and disadvantages of each. We also describe a new transgenic model that enables highly efficient gene delivery into primary T cells by nonreplic ating adenoviral vectors.

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Authors and Affiliations

  1. Department of Pathology, University of Alabama at, 35233-2170, Birmingham, Al, UK

    Vincent Hurez, Robin D. Hatton & James Oliver

  2. Department of Medicine, University of Wales College of Medicine, Cardiff, UK

    R. James Matthews & Casey T. Weaver

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  1. Vincent Hurez
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  2. Robin D. Hatton
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  3. James Oliver
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  4. R. James Matthews
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  5. Casey T. Weaver
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Correspondence to Casey T. Weaver.

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Hurez, V., Hatton, R.D., Oliver, J. et al. Gene delivery into primary T cells. Immunol Res 26, 131–141 (2002). https://doi.org/10.1385/IR:26:1-3:131

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