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The interface between tapasin and MHC class I

Identification of amino acid pesidues in both proteins that influence their interaction

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Abstract

Prior to the binding of antigenic peptide, a complex of chaperone proteins associates with the Major Histocompatibility Complex (MHC) class I heavy chain/β2m heterodimer. Although each dornain of the MHC class I heavy chain contains amino acid resid uses that influence chaperone binding, there are several pieces of evidence that point to an interaction between the MHC clas 1α2/α3 domains and tapasin. In egard to the site on tapasin involved in the tapasin/MHC interface, we have found that a particular region of tapasin (containing amino acid residues 334–342) is necessary for the binding of tapasin to the MHC class I heavy chain. Our results also indicate that amino acids in this region of tapasin also affect the proportion of MHC class I open forms expressed at the cell surface and MHC class I egress from the endoplasmic reticulurn. Based on these results and those obtained by other laboratories, a model for MHC class I/tapasin interaction is proposed.

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Authors and Affiliations

  1. Eppley Institute for Research in Cancer and Allied Diseases, Department of Pathology and Microbiology, University of Nebraska Medical Center, 986805 Nebraska Medical Center, 68198-6805, Omaha, NE

    Hēth R. Turnquist, Shanna E. Vargas, Erin L. Schenk, Mary M. McIlhaney, Adrian J. Reber &  Joyce C. Solheim

  2. Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, 986805 Nebraska Medical Center, 68198-6805, Omaha, NE

    Hēth R. Turnquist, Shanna E. Vargas, Erin L. Schenk, Mary M. McIlhaney, Adrian J. Reber &  Joyce C. Solheim

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  1. Hēth R. Turnquist
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  2. Shanna E. Vargas
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Turnquist, H.R., Vargas, S.E., Schenk, E.L. et al. The interface between tapasin and MHC class I. Immunol Res 25, 261–269 (2002). https://doi.org/10.1385/IR:25:3:261

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