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Pituitary adenylate cyclase-activating polypeptide does not colocalize with vasoactive intestinal polypeptide in the hypothalamic magnocellular nuclei and posterior pituitary of cats and rats

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Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal polypeptide (VIP) immunoreactive cells were demonstrated in the hypothalamic magnocellular nuclei in cats and rats. In cats these immunoreactive cells were stained without any treatment or intervention; however, in rats we had to use the pituitary stalk section to enhance the amount of PACAP and VIP for successful immunostaining. In both species the regions occupied by PACAP and VIP immunoreactive cells partially overlap each other in the paraventricular and supraoptic nuclei. Nevertheless, in either cats or rats PACAP and VIP immunoreactivities do not colocalize in the same cells studied by double labeling immunohistochemistry (IHC) or by the combination of immunohistochemistry and in situ hybridization. As was expected, PACAP and VIP immunoreactive materials were stored in different fibers of the posterior pituitary where the distribution of PACAP and VIP fibers also showed different patterns: PACAP fibers form a dense plexus at the periphery of the posterior lobe, in the vicinity of the intermediate lobe; however, the VIP fibers were evenly distributed mainly in the center of the posterior lobe. In spite of the high sequence homology of PACAP and VIP, the two peptides are synthesized in different subpopulations of hypothalamic neurons. This different distribution correlates well with the different role of the hypothalamic PACAP and VIP in the biologic clock and in the functions of the anterior and posterior pituitary.

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Authors and Affiliations

  1. Department of Anatomy, Histology and Embrylogy, Hungary

    Viktoria Vereczki

  2. Division of Neuromorphology, Hungarian Academy of Sciences, Budapest, Hungary

    Zsuzsanna E. Tóth

  3. Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University, Japan

    Akemichi Baba & Hitoshi Hashimoto

  4. Department of Surgery, St. John Hospital, Budapest, Hungary

    Kristóf Fógel

  5. US-Japan Biomedical Research Laboratories, Tulane University Hebert Center, Belle Chasse, New Orleans, LA

    Akira Arimura

  6. Department of Medicine, Tulane University School of Medicine, New Orleans, LA

    Akira Arimura

  7. Department of Human Morphology and Developmental Biology, Faculty of Medicine, Semmelweis University, Tuzoltó u. 58, H-1094, Budapest, Hungary

    Katalin Köves &  Mária Kausz

Authors
  1. Viktoria Vereczki
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  2. Katalin Köves
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  3. Zsuzsanna E. Tóth
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  4. Akemichi Baba
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  5. Hitoshi Hashimoto
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  6. Kristóf Fógel
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  7. Akira Arimura
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  8. Mária Kausz
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Correspondence to Mária Kausz.

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Vereczki, V., Köves, K., Tóth, Z.E. et al. Pituitary adenylate cyclase-activating polypeptide does not colocalize with vasoactive intestinal polypeptide in the hypothalamic magnocellular nuclei and posterior pituitary of cats and rats. Endocr 22, 225–237 (2003). https://doi.org/10.1385/ENDO:22:3:225

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  • DOI: https://doi.org/10.1385/ENDO:22:3:225

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