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Retinoid-related receptor (ROR) α mRNA expression is altered in the brain of male mice lacking all ligand-binding thyroid hormone receptor (TR) isoforms

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Abstract

In the vertebrate brain, the thalamus serves as a relay and integration station for diverse neuronal information en route from the periphery to the cortex. Deficiency of TH during development results in severe cerebral abnormalities similar to those seen in the mouse when the retinoic acid receptor (ROR)α gene is disrupted. To investigate the effect of the thyroid hormone receptors (TRs) on RORα gene expression, we used intact male mice, in which the genes encoding the α and β TRs have been deleted. In situ hybridization for RORα mRNA revealed that this gene is expressed in specific areas of the brain including the thalamus, pons, cerebellum, cortex, and hippocampus. Our quantitative data showed differences in RORα mRNA expression in different subthalamic nuclei between wild-type and knockout mice. For example, the centromedial nucleus of the thalamus, which plays a role in mediating nociceptive and visceral information from the brainstem to the basal ganglia and cortical regions, has less expression of RORα mRNA in the knockout mice (−37%) compared to the wild-type controls. Also, in the dorsal geniculate (+72%) and lateral posterior nuclei (+58%) we found more RORα mRNA in dKO as compared to dWT animals. Such differences in RORα mRNA expression may play a role in the behavioral alterations resulting from congenital hypothyroidism.

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Author information

Author notes

  1. Douglas Forrest

    Present address: National Institutes of Health, NIDDK, CEB, Bldg. 10, 10 Center Drive, MSC 1770, 20892-1770, Bethesda, MD

Authors and Affiliations

  1. Laboratory of Neurobiology and Behavior, The Rockefeller University, 1230, York Ave, 10021, New York, NY

    Nandini Vasudevan, Hosein Kami Kia & Donald Pfaff

  2. Neuropsychology Program, Department of Psychology, Queens College/CUNY, 65-30 Kissena Blvd, 11367, Flushing, NY

    Maria Hadjimarkou

  3. Dokkyo University School of Medicine Mibu, 321-0293, Tochigi, Japan

    Noriyuki Koibuchi

  4. Lilly Corporate Center, Building 98c/2, Room 2370, 46285, Indianapolis, IN

    William W. Chin

  5. Department of Human Genetics, Mount Sinai School of Medicine, 10029, New York, NY

    Douglas Forrest

  6. Laboratory of Developmental Biology, CMB, Karolinska Institute, S-17 177, Stockholm, Sweden

    Bjorn Vennstrom

Authors
  1. Nandini Vasudevan
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  2. Hosein Kami Kia
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  4. Noriyuki Koibuchi
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Correspondence to Nandini Vasudevan.

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The first three authors contributed equally to this work.

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Vasudevan, N., Kia, H.K., Hadjimarkou, M. et al. Retinoid-related receptor (ROR) α mRNA expression is altered in the brain of male mice lacking all ligand-binding thyroid hormone receptor (TR) isoforms. Endocr 26, 25–32 (2005). https://doi.org/10.1385/ENDO:26:1:025

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  • DOI: https://doi.org/10.1385/ENDO:26:1:025

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